FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853653
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAAGACAGGCCAGGCCCCCGTATTGGTGATATATAACGACAATGAGAGGCCCTCGGGGATCCCTGAGCGATT35130.4115255261798412No Hit
GTCTGTGAGGCCTCCTCCGAGCTGACACAGCCAGCCTCGGTGTCGGTGTCCCCAGGACAGACGGCCAAGATCAC32870.3850510687597888No Hit
GTCTTATGTTGGTACCAATAAACATACTGTCTTGCCAGTCCATCTCCAGAGCAGGTGATCTTGGCCGTCTGTC24850.29110188800367365No Hit
CTCTCTGCACAGTCTGTGAGGCCTCCTCCGAGCTGACACAGCCAGCCTCGGTGTCGGTGTCCCCAGGACAGACGG22510.26369028164839814No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21750.2547873667637787No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21140.24764160613270264No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20980.24576730826225643No Hit
CCCCAGGACAGACGGCCAAGATCACCTGCTCTGGAGATGGACTGGCAAGA19660.2303043508310754No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19170.22456431360283394No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16240.19024123385028813No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCGC15960.1869612125770073No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15720.184149765771338No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15450.18098688811496005No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT15350.17981545194593118No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14600.17102968067821467No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14560.17056110621060314No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14380.16845252110635117No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC14110.1652896434499732No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13930.16318105834572127No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12630.147952388148346No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12480.14619523389480268No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12460.1459609466609969No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12350.14467236687506516No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12250.14350093070603628No Hit
CTATTATTGTCAATCAACAGACAGCGGTGGTGATGCCTTGGTAATCTTCGGCGGCGGGACCAAGCTGACCGTC12080.14150948921868722No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11870.13904947326372658No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11620.13612088284115442No Hit
GCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCGCCGCCGAAGATTAC11220.13143513816503896No Hit
GGCCAGGCCCCCGTATTGGTGATATATAACGACAATGAGAGGCCCTCGGG11220.13143513816503896No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11180.1309665636974274No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11020.12909226582698122No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10950.12827226050866103No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10680.12510938285228307No Hit
ACATGACTGTTGTCCCTAATTTGGAGCCAGAGAATCGCTCAGGGATCCCC10640.12464080838467152No Hit
CAGTCATGTTGACCATCAGTGGGGTCCAGACAGAAGACGAGGCTGACTAT10510.12311794136493401No Hit
CTATATACACTGGGTGCGTCAGGCCCCTGGACAAGGGTTTGAATGGATGGGAATGATCAATCCTAGTATTGCTGG10340.12112649987758492No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10320.12089221264377915No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT10310.12077506902687625No Hit
GTCTGGACCCCACTGATGGTCAACATGACTGTTGTCCCTAATTTGGAGCC9940.11644075520146945No Hit
GGTCCAGACAGAAGACGAGGCTGACTATTATTGTCAATCAACAGACAGCGGTGGTGATGCCTTGGTAATCTTCGG9630.11280930307747995No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG9630.11280930307747995No Hit
GTCCTGCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9460.11081786159013089No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9230.10812355840136448No Hit
TCCTCACTCTCTGCACAGTCTGTGAGGCCTCCTCCGAGCTGACACAGCCA9100.10660069138162696No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9080.10636640414782118No Hit
GCTCTGGAGATGGACTGGCAAGACAGTATGTTTATTGGTACCAACATAAG8900.10425781904356923No Hit
GTATGTTTATTGGTACCAACATAAGACAGGCCAGGCCCCCGTATTGGTGA8840.10355495734215191No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8750.10250066479002592No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8570.10039207968577396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT150.00246382268.2207934
AGGGGGG3200.055.11083670
GCTTATC252.821863E-454.151351
TGCGTTA200.006882381652.63252649
CGGGATA200.00794170650.76094417
GGCGAAT2600.048.44159332
TCGTATG2600.047.71438240
GACTAAG2700.047.69460726
CGTATGC2700.046.02681441
GTATGCC2750.045.23591642
TCTCGTA2600.044.89752638
CCCACGA2900.044.3428919
GCGAATC2650.043.7255533
GGTATCA10550.043.629051
ACTAAGG3000.042.94782327
AGACTAA3000.042.90752425
ATCTCGT2650.042.67870337
CACGAGA3050.042.16456221
ATGCCGT2800.042.14944
TAAGGCG3100.041.62103729
Produced by FastQC (version 0.11.5)