FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853653
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32780.38399677620766287No Hit
ACATAAGACAGGCCAGGCCCCCGTATTGGTGATATATAACGACAATGAGAGGCCCTCGGGGATCCCTGAGCGATT29950.3508451326241459No Hit
GTCTGTGAGGCCTCCTCCGAGCTGACACAGCCAGCCTCGGTGTCGGTGTCCCCAGGACAGACGGCCAAGATCAC29480.3453393826297102No Hit
GTCTTATGTTGGTACCAATAAACATACTGTCTTGCCAGTCCATCTCCAGAGCAGGTGATCTTGGCCGTCTGTC23130.2709531858963771No Hit
CTCTCTGCACAGTCTGTGAGGCCTCCTCCGAGCTGACACAGCCAGCCTCGGTGTCGGTGTCCCCAGGACAGACGG21980.2574816699525451No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21070.24682160081438242No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20460.23967584018330634No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20040.2347558082733851No Hit
GCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCGCCGCCGAAGATTAC18540.21718426573795208No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18250.21378710084776834No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17900.2096870742561673No Hit
CCCCAGGACAGACGGCCAAGATCACCTGCTCTGGAGATGGACTGGCAAGA16630.19480983490950068No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCGC15610.18286118598540624No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14520.1700925317429916No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14350.16810109025564252No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14050.16458678174855593No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14050.16458678174855593No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC13360.15650387218225673No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13280.15556672324703363No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12970.15193527112304414No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12250.14350093070603628No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12190.14279806900461897No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11670.13670660092566886No Hit
CTATTATTGTCAATCAACAGACAGCGGTGGTGATGCCTTGGTAATCTTCGGCGGCGGGACCAAGCTGACCGTC11150.13061513284671875No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11060.12956084029459278No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11040.129326553060787No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10700.12534367008608882No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10540.12346937221564265No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10430.1221807924297109No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10140.11878362753952718No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9990.11702647328598388No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT9920.11620646796766368No Hit
CTATATACACTGGGTGCGTCAGGCCCCTGGACAAGGGTTTGAATGGATGGGAATGATCAATCCTAGTATTGCTGG9880.11573789350005213No Hit
GGCCAGGCCCCCGTATTGGTGATATATAACGACAATGAGAGGCCCTCGGG9870.11562074988314924No Hit
ACATGACTGTTGTCCCTAATTTGGAGCCAGAGAATCGCTCAGGGATCCCC9710.11374645201270306No Hit
CAGTCATGTTGACCATCAGTGGGGTCCAGACAGAAGACGAGGCTGACTAT9190.10765498393375293No Hit
GTCTACGTGGAGCTGAGCGGCCTAACATCTGACGACACGGCCGAATATTA9140.1070692658492385No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9040.10589782968020965No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8980.10519496797879231No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8810.10320352649144324No Hit
GTCCTGCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8810.10320352649144324No Hit
GGTCCAGACAGAAGACGAGGCTGACTATTATTGTCAATCAACAGACAGCGGTGGTGATGCCTTGGTAATCTTCGG8730.10226637755622016No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8660.10144637223789994No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8620.10097779777028841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11750.047.20371
GTGGTAT2900.041.9831051
TGGTATC3050.039.887962
GTATCAA28850.037.9812431
AATGGTA2453.6379788E-1233.10429470
TATCAAC33650.032.5386162
AACGCAG35750.030.4364196
TCAACGC36050.030.1795964
ATCAACG36350.029.9305233
CAACGCA36450.029.7574545
ACGCAGA37200.029.2500537
CGCAGAG37750.028.7343798
GTGCTAG1302.4920155E-1028.6166881
AGAGTAC37600.028.57939311
CAGAGTA38000.028.54533610
GCAGAGT38800.027.9567749
CCGAGTG550.00808556127.34920367
GCTATAC500.00856198327.0304223
ACCCGCG659.5440086E-426.09779429
AACATAA10550.025.62575770
Produced by FastQC (version 0.11.5)