FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803150
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25200.31376455207619997No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20240.25200771960405904No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGTAAAGTGTACCTGCCTGCATCTGAGCAG19690.24515968374525307No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18910.23544792380003735No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18800.2340783166282762No Hit
CCTATGAACATTCTGAAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18700.23283321919940234No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18660.23233518022785282No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGTAAAGTGTACCTGCCTGCATCTGAGCAGCAGTAATAAT16340.20344891987798047No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAA16200.20170578347755713No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16170.20133225424889498No Hit
GATTATTACTGCTGCTCAGATGCAGGCAGGTACACTTTACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT16030.1995891178484716No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT15980.1989665691340347No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15820.19697441324783663No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15490.19286559173255308No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15170.18888127996015688No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14680.18278030255867522No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14270.1776754031002926No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12850.1599950196102845No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12850.1599950196102845No Hit
CTCCTGCACTGGAACCAACAATGATGTTGGTGCTTCTAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC12570.15650874680943783No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12510.15576168835211354No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12430.15476561040901451No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12170.1515283570939426No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCAGAT12090.15053227915084355No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12020.14966071095063188No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11920.14841561352175808No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11670.14530286994957356No Hit
CTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAACAATGATGTT11520.14343522380626283No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11370.14156757766295214No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11210.13957542177675403No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT10980.13671169769034427No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10920.13596463923301996No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG10880.13546660026147048No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCGC10830.13484405154703355No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG10550.1313577787461869No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10340.1287430741455519No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG10220.12724895723090332No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9860.1227666064869576No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9850.12264209674407021No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCGCCATCTCCTG9600.11952935317188569No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9570.11915582394322356No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9420.11728817779991284No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9230.11492249268505261No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9180.11429994397061569No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9000.11205876859864283No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8990.11193425885575546No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8790.10944406399800784No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8560.10658033991159808No Hit
GTTGAGAACCTATGAACATTCTGAAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8530.10620681068293594No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8470.10545975222561166No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8460.10533524248272426No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGAAGGGGCCA8440.10508622299694952No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA8220.10234700865342714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGT100.009886375129.7327970
AGGGGGG3250.051.89311670
ATATCGT200.00746674251.5586937
GGTATCA9250.043.0622751
TCGTATG2700.040.92040340
CGTATGC2800.040.76671641
GTATCAA24900.040.1976661
ATGCCGT2950.040.00304844
ATCTCGT2500.038.49715837
TCTCGTA2600.038.37953638
GTATGCC3250.038.35626642
ACGAGAC3050.037.9423122
GCTTGTA451.0827111E-437.8277361
ATCTCCG3250.036.65239310
CACGAGA3200.036.1615121
CGGGTCA853.3887773E-936.03638517
GTGGTAT2650.035.9720341
CGAGACA3250.035.61404823
CTTATAC3450.035.525181
TCTCCGA3450.035.51412211
Produced by FastQC (version 0.11.5)