FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803150
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34520.42980763244723896No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24030.2991969121583764No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19370.24117537197285688No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18650.23221067048496544No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18180.22635871256925852No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGTAAAGTGTACCTGCCTGCATCTGAGCAG18130.22573616385482162No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18120.2256116541119343No Hit
CCTATGAACATTCTGAAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16960.2111685239369981No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAA16640.20718421216460187No Hit
GATTATTACTGCTGCTCAGATGCAGGCAGGTACACTTTACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT15980.1989665691340347No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15740.19597833530473763No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGTAAAGTGTACCTGCCTGCATCTGAGCAGCAGTAATAAT14920.18576853638797236No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14850.1848969681877607No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14430.1796675589864907No Hit
CTCCTGCACTGGAACCAACAATGATGTTGGTGCTTCTAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC13640.16983128929838762No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13500.16808815289796425No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13290.16547344829732927No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12500.15563717860922618No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCAGAT12380.15414306169457762No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11970.14903816223619498No Hit
CTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAACAATGATGTT11930.14854012326464544No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11890.14804208429309593No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG11880.14791757455020854No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11860.1476685550644338No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11710.14580090892112307No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11280.1404469899769657No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11210.13957542177675403No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11070.1378322853763307No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCGC11010.13708522691900643No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10960.1364626782045695No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10820.13471954180414616No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT10530.13110875926041213No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCGCCATCTCCTG10420.12973915208865094No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10110.1258793500591421No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9570.11915582394322356No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG9520.11853327522878665No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9170.11417543422772831No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9160.11405092448484094No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG9150.11392641474195356No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9040.11255680757019236No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8940.11131171014131856No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8900.11081367116976903No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8700.10832347631202141No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8580.10682935939737284No Hit
GTTGAGAACCTATGAACATTCTGAAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8420.10483720351117476No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8360.10409014505385046No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC8330.10371661582518832No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGAAGGGGCCA8240.10259602813920189No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8040.10010583328145428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.040.0983161
CGTTAAG350.001418082339.02157229
GTATCAA23950.038.201331
TGGTATC2950.035.7124562
TATCAAC28600.032.0832062
GTGGTAT3300.031.9506321
TTGCAAT1053.0774885E-431.66234470
ATCAACG29500.031.1005233
TACTCGC450.00435790531.03741848
ACGGGGT1650.030.90648716
TCAACGC30000.030.5821844
AACGCAG30300.030.2812736
GCTAAGC450.00497658730.2065059
CAACGCA30400.030.1816625
CGCAGAG31450.029.065968
ACGTGAT1155.244088E-428.90909670
AGAGTAC31950.028.61109211
GACAGGT1551.8189894E-1228.5261651
CAGAGTA32200.028.38895410
GCAGAGT32150.028.3274089
Produced by FastQC (version 0.11.5)