FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651140
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT33900.520625364744909No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21990.33771539146727275No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21910.3364867770371963No Hit
TAGTTATACTGCTGGCAGTAATAAGTTGCAAAATCATCAGCCTGCAGACTGCTTATGGTGAGAGCGAAATCCGTC17740.2724452498694597No Hit
GTATAACTATTATCCATTTACGTTCGGCCCTGGGACCAAAGTGGAGATCAGACGAACTGTGGCTGCACCAT16780.25770187670854194No Hit
GTCTGGGGCTGAGTTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTA14900.22882943760174465No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG14720.22606505513407255No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14300.219614829376171No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14070.21608256288970112No Hit
GGCTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAGGCCCCTACACTCCTGATCTACAAGGCGT13550.20809656909420404No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12740.19565684798967964No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12730.19550327118592006No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12590.19335319593328623No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12340.18951377583929724No Hit
CACCATAAGCAGTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAGTATAACTATTATCCATTTACGTT11970.1838314341001935No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11840.18183493565131922No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11790.18106705163252143No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11600.17814909236108978No Hit
ATAGTAGACTGCCGAATCTTCAACTGCCAGACTGTTCAGTTCCATGTAGA11350.17430967226710078No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGCTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAA11220.1723131738182265No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10450.16048775992874034No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10440.1603341831249808No Hit
CCATAAGCAGTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAG10390.159566299106183No Hit
GCTTATGGTGAGAGCGAAATCCGTCCCAGATGCACTGCCGCTGAACCTTGACGGGACCCCATTCTCCAAATTAGA10070.1546518413858771No Hit
CTCCTGATCTACAAGGCGTCTAATTTGGAGAATGGGGTCCCGTCAAGGTT9880.15173388211444544No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9860.1514267285069263No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9770.15004453727309028No Hit
GTGTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTACTA9120.14006204502871886No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8960.1376048161685659No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8930.1371440857572872No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8900.13668335534600853No Hit
CCCCTACACTCCTGATCTACAAGGCGTCTAATTTGGAGAATGGGGTCCCG8780.13484043370089382No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT8710.1337653960745769No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8640.13269035844826No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8500.13054028319562613No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8280.1271615935129158No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCGACA8230.12639370949411802No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8160.12531867186780107No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACTTTGGTCC8000.12286144300764812No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7930.1217864053813312No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7870.12086494455877383No Hit
GTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAGTATAACTATTATCCATTTACGTTCGGCCCTGGGA7840.12040421414749515No Hit
GTCTACTATTGTGCGAACGAAAGGGGTGGCCTCTACGACTACTGGGGTCA7800.11978990693245692No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7790.11963633012869737No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7700.11825413889486132No Hit
CCTTCGACACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG7610.11687194766102527No Hit
CTATTATCCATTTACGTTCGGCCCTGGGACCAAAGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCAT7540.11579691003470835No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7160.10996099149184507No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7150.1098074146880855No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7080.10873237706176858No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7050.1082716466504899No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACTTTGGTCCCAGGGCCGAACGTA7050.1082716466504899No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6990.10735018582793256No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6890.10581441779033696No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6840.10504653377153915No Hit
GAACTGAACAGTCTGGCAGTTGAAGATTCGGCAGTCTACTATTGTGCGAACGAAAGGGGTGGCCTCTACGACTA6800.1044322265565009No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6780.10412507294898178No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6640.10197499769634795No Hit
CTAATAGCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA6630.10182142089258839No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6550.1005928064625119No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6530.10028565285499279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT1000.050.229081
CTTATAC5700.046.997971
ATCTCGT5300.045.2205737
TCGTATG6000.044.89439840
ATGCCGT5950.044.6378744
CGTATGC5900.044.6332141
TATACAC6000.044.624073
GTATGCC5950.044.34981542
TTATACA6050.044.255282
GGATCTC5400.044.1680235
GCCGTCT5950.043.70500646
CTTGAAA6400.043.6701257
CGAGACA6150.043.55574823
CTGCTTG6200.043.5452954
CGTCTTC6200.042.71269648
ACGAGAC6300.042.51217722
TATGCCG6250.042.34923643
ATACACA6400.041.8350684
CACGAGA6350.041.64381821
GCTTGAA6700.041.57278456
Produced by FastQC (version 0.11.5)