FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651140
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63950.9821236600423873No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22990.3530730718432288No Hit
TAGTTATACTGCTGGCAGTAATAAGTTGCAAAATCATCAGCCTGCAGACTGCTTATGGTGAGAGCGAAATCCGTC19900.3056178394815247No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17890.2747489019258531No Hit
GTATAACTATTATCCATTTACGTTCGGCCCTGGGACCAAAGTGGAGATCAGACGAACTGTGGCTGCACCAT17210.26430567927020304No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15170.23297601130325274No Hit
GTCTGGGGCTGAGTTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTA13720.21070737475811654No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13210.20287495776637895No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG13040.20026415210246643No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12160.18674939337162516No Hit
GCTTATGGTGAGAGCGAAATCCGTCCCAGATGCACTGCCGCTGAACCTTGACGGGACCCCATTCTCCAAATTAGA11980.18398501090395308No Hit
GGCTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAGGCCCCTACACTCCTGATCTACAAGGCGT11940.1833707036889148No Hit
CACCATAAGCAGTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAGTATAACTATTATCCATTTACGTT11730.18014559080996406No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11600.17814909236108978No Hit
ATAGTAGACTGCCGAATCTTCAACTGCCAGACTGTTCAGTTCCATGTAGA11390.17492397948213903No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11320.17384894185582211No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11000.16893448413551618No Hit
CTCCTGATCTACAAGGCGTCTAATTTGGAGAATGGGGTCCCGTCAAGGTT10540.1618699511625764No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10160.15603403261971313No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10120.15541972540467486No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10090.1549589949933962No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9710.1491230764505329No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGCTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAA9690.1488159228430138No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9660.1483551924317351No Hit
CCATAAGCAGTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAG9650.14820161562797554No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9460.1452836563565439No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8990.13806554657984457No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8850.13591547132721074No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8720.13391897287833646No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8520.13084743680314526No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8480.130233129588107No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8410.1291580919617901No Hit
CCCCTACACTCCTGATCTACAAGGCGTCTAATTTGGAGAATGGGGTCCCG8370.12854378474675185No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8250.12670086310163714No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8230.12639370949411802No Hit
GTGTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTACTA8100.12439721104524372No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7950.12209355898885033No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT7890.12117209816629297No Hit
GTCTACTATTGTGCGAACGAAAGGGGTGGCCTCTACGACTACTGGGGTCA7860.12071136775501429No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7790.11963633012869737No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACTTTGGTCCCAGGGCCGAACGTA7620.11702552446478484No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCGACA7550.11595048683846793No Hit
CTATTATCCATTTACGTTCGGCCCTGGGACCAAAGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCAT7320.11241822035199804No Hit
GTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAGTATAACTATTATCCATTTACGTTCGGCCCTGGGA7290.11195748994071936No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7240.11118960592192155No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7060.10842522345424947No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6830.10489295696777959No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACTTTGGTCC6790.10427864975274136No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6760.10381791934146267No Hit
CCTTCGACACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG6740.10351076573394355No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6730.10335718893018399No Hit
GAACTGAACAGTCTGGCAGTTGAAGATTCGGCAGTCTACTATTGTGCGAACGAAAGGGGTGGCCTCTACGACTA6650.1021285745001075No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6560.10074638326627147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG451.9404033E-674.6191870
TCGCATA251.6135658E-460.63247366
CGCATAC351.204983E-554.85859367
GCATACG402.0852285E-550.00316268
CATACGG452.2117374E-549.50636769
GTGGTAT2350.041.230351
GTATCAA16750.039.2950941
GCTTGTA350.00157746538.1837731
CGGTCGC400.001804957337.15009363
CACTTTT7150.032.87418470
TATCAAC20000.032.5430032
ATCAACG20650.031.6705513
TCAACGC21000.031.1427084
AACGCAG21500.030.728856
CAACGCA21450.030.4893635
GTCGCAT500.005143295530.00224165
AACTGTA900.00527143229.84767270
GGTCGCA500.00529259729.82718364
ACGCAGA22400.029.4942077
CGCAGAG22650.029.1686678
Produced by FastQC (version 0.11.5)