FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754454
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31640.4193761316130606No Hit
GTATATTCACTGGCCTCCGAGGTACACTTTTGGCCAGGGGACCAAGTTGG27380.3629114564970164No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26200.3472710065822436No Hit
GTGAATATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT25780.3417040667820702No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23950.31744811479560053No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20230.2681409337083507No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT18360.24335479697900733No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18260.2420293351218232No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17760.23540202583590253No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16620.22029176066400338No Hit
GTGTTAGCACCAGCTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTACGGTGCTTCCA16190.2145922746781116No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15480.20518149549210424No Hit
GTCCCAGACCCAGTGCCACTGAACCTGGCTGGGATACCCGTGGCCCTGGT15350.20345839507776484No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTTAGCACCAGCTTAGCCTGGTA15190.20133765610627022No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATATTCACTGG15020.1990843709490572No Hit
CTCTTGCAGGGCCAGTCAGAGTGTTAGCACCAGCTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCAGGCT14330.18993868413448667No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14260.18901086083445778No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14100.18689012186296314No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13730.18198591299138184No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13660.18105808969135295No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTTAGCAC13640.18079299731991613No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13030.17270767999109288No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12360.1638270855479592No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT12200.16170634657646457No Hit
CCACTGGAGAGATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCT11990.1589228766763779No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11960.15852523811922264No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11650.1544163063619518No Hit
GTTTATTACTGTCAGCAGTATATTCACTGGCCTCCGAGGTACACTTTTGGCCAGGGGACCAAGTTGGAGATCACA11280.14951209749037053No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11130.1475239047045943No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11040.1463309890331286No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11030.14619844284741018No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10770.14275224201873143No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCACACGAACTGTGGCTGCACCA10660.14129423397582888No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAACTTGGTCCCCTGGCCAAAAGTGTACCTCGGAGGCCAGTGAATATA10580.14023386449008157No Hit
CCTCTATATCTTTTGCACAGAAATACAAGGCGGTGTCCTCAGTTCTCAGA10540.13970367974720793No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATCTCCAACTTGGTCCCCTGGCCAAAAGTGTACCTCGGAGG10500.13917349500433426No Hit
GTCAGAGTGTTAGCACCAGCTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTACGGTG10350.13718530221855804No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10340.13705275603283965No Hit
CATTTACGGTGCTTCCACCAGGGCCACGGGTATCCCAGCCAGGTTCAGTGGCACTGGGTCTGGGACAGAGTTCA10280.13625747891852916No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9890.13108817767551104No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9770.12949762344689006No Hit
GGCCAAAAGTGTACCTCGGAGGCCAGTGAATATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGG9710.1287023463325796No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9620.12750943066111387No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9550.12658160736108498No Hit
AGCTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCAGGCTCCTCAT9540.12644906117536656No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9510.1260514226182113No Hit
GCCACAGTTCGTGTGATCTCCAACTTGGTCCCCTGGCCAAAAGTGTACCTCGGAGGCCAGTGAATATACTGCTGA9290.12313540653240622No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9240.12247267560381415No Hit
GCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTACGGTGCTTCCACCAGGGCCACGGGTATC9090.12048448281803793No Hit
GAGTGAACTCTGTCCCAGACCCAGTGCCACTGAACCTGGCTGGGATACCC9070.12021939044660113No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8960.11876138240369857No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8890.11783355910366967No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8890.11783355910366967No Hit
CTCCTGTGCAGCCTCCGGATTGAACTTTGATGATTATGCCATGCACTGGGTCCGTCAAGCTCCGGGAAAGGGT8720.11558027394645665No Hit
CTATGCAGACTCTGTGAAGGGTCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCCTGTATCTGCACATGAA8680.11505008920358298No Hit
GCTGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTACGGTGCTTCCACCAGGGCCACGGGTATCCCAGCCAGGTT8480.11239916548921472No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCAGTGCCA8270.10961569558912802No Hit
ATATAGAGGAGGGCGGTTTTGCCAGAGGTCACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG8250.10935060321769122No Hit
GTCTGAGAACTGAGGACACCGCCTTGTATTTCTGTGCAAAAGATATAGAG8250.10935060321769122No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8190.10855532610338071No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8100.107362410431915No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTTTTTTAAAAGGTGTCCAGTGTGAAGTGCAACTGGTGGAGT8050.10669967950332293No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7850.10404875578895464No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7810.103518571046081No Hit
GGCCACGGGTATCCCAGCCAGGTTCAGTGGCACTGGGTCTGGGACAGAGT7770.10298838630320734No Hit
CTCCCAGGCTCCTCATTTACGGTGCTTCCACCAGGGCCACGGGTATCCCA7650.10139783207458639No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7570.10033746258883908No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGA200.00746124351.56798637
AATATCG200.00748894751.51967236
GCGATAG200.00748894751.51967236
TCGTATG2700.046.0417640
GCCGTCT2650.045.9692746
CGTATGC2650.045.64425341
CTTATAC2700.044.1333241
GGTATCA9100.043.3989221
ATCTCGT2550.043.14184637
TATACAC2850.042.9680443
GTATGCC3000.042.68627542
CACGAGA2800.042.53455721
ACGAGAC3000.040.83317622
TCTCCGA3000.040.81964511
ATCTCCG3050.040.1504710
AGGCAAT3000.039.89996732
CGTCTTC3250.039.7484948
TTATACA3000.039.6857642
CGAGACA3100.039.51597623
AATGGTA2000.039.198570
Produced by FastQC (version 0.11.5)