FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338423
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT63720.47608267341490695No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57090.4265467643637325No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54060.40390818149419133No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA51280.3831374684983746No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT45650.3410730389420983No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCTGTGCTCGTGGATGTGTCTGTGGT40670.30386507105750576No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32940.2461105345619434No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32750.24469095345791275No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30390.22705826184995326No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG30290.22631111390046346No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT30010.224219099641892No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29950.22377081087219813No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29390.2195867823550552No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT29260.2186154900207184No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGGCTGGTA28970.21644876096719798No Hit
CTGGTATCCCAGCCAAGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT28650.21405788752883056No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28550.21331073957934077No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28470.21271302121974892No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28320.21159229929551418No Hit
GTCCCAGACCCACTGCCACTGAACTTGGCTGGGATACCAGTGGCCCTGGT27800.20770712995816717No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC27760.2074082707783713No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG27130.20270123869658546No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26780.20008622087337113No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26120.1951550444067384No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA24560.18349953639469732No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24490.18297653283005447No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT24300.18155695172602382No Hit
GTGTATTACTGTGCGAGAGAAGTATTGTACCCCGACTACTGGGGCCAGGG24190.18073508898158505No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23800.17782121197857478No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT23720.17722349361898293No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23250.17371189825638084No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCAGTACACTTTTGGCCA22840.1706485916634726No Hit
AAAGTGTACTGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAA22700.16960258453418686No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22150.16549327081199292No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG22070.16489555245240106No Hit
GTCAGAGTGTTAGCAGCAACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTG21820.16302768257867656No Hit
GTATTGTACCCCGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT21500.16063680914030917No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21410.15996437598576832No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACTTGGCTGGGATACCA21290.15906779844638055No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20540.153464188825207No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20450.15279175567066614No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20000.149429589897962No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA19820.14808472358888033No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGTCTTCTGGTTACACCTTTATCAA19810.14801000879393136No Hit
GGCCAAAAGTGTACTGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTG19760.14763643481918645No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19510.14576856494546195No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACTGAGGCCAGTTATTATACTG19300.14419955425153333No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18790.1403890997091353No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATGGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGC18730.1399408109394414No Hit
TCCATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAAGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT18660.13941780737479853No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18490.13814765586066588No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18400.13747522270612503No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18250.13635450078189035No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18010.1345613457031148No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17580.1313486095203086No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17370.12977959882638002No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17220.12865887690214528No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17110.12783701415770649No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTA16960.12671629223347178No Hit
CTGCTACTCTGGCTCCCAGATATCACTGGAGAAATGGTGATGACGCAGTC16700.12477370756479828No Hit
CTCCCAGGTTCAACTGGTGCAGTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGT16580.12387713002541051No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAG16360.12223340453653292No Hit
GTGTTAGCAGCAACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATCCA16340.12208397494663495No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16200.12103796781734924No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16160.1207391086375533No Hit
GTACAATACTTCTCTCGCACAGTAATACACGGCCGTGTCGTCAGATCTCA16070.12006667548301247No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15990.11946895712342062No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCACTTACAATGGT15820.11819880560928794No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAAGTT15780.11789994642949203No Hit
GCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTA15600.11655508012041037No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC15540.11610679135071648No Hit
GTGCATCCACCAGGGCCACTGGTATCCCAGCCAAGTTCAGTGGCAGTGGG15540.11610679135071648No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15510.11588264696586953No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15400.11506078422143075No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT15340.11461249545173686No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15160.11326762914265519No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15140.11311819955275725No Hit
GAGTGTTAGCAGCAACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATC15110.11289405516791029No Hit
GTATTACTGTGCGAGAGAAGTATTGTACCCCGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATC15110.11289405516791029No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGG15090.11274462557801232No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15060.11252048119316539No Hit
GGAGAAATGGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG15000.11207219242347151No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT14890.1112503296790327No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14830.11080204090933882No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT14790.1105031817295429No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA14750.11020432254974696No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC14610.10915831542046125No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACTGGAGAAATGGTGATGACGCAGTCTCCAGCCACC14580.1089341710356143No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14530.1085605970608694No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14530.1085605970608694No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACTGGAGAAATGGTGATGACGCAGTCTC14530.1085605970608694No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14390.10751458993158367No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14340.10714101595683877No Hit
GGTTTCTGCTGGTACCAGCCTAAGTTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC14210.10616972362250202No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGGCTGGTACCAGCAGAAACCTGGCCAGG14180.10594557923765506No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14120.10549729046796118No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14050.10497428690331831No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14020.10475014251847135No Hit
CAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13910.10392827977403257No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13900.10385356497908359No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13890.1037788501841346No Hit
CTCCCAGGCTCCTCATCCATGGTGCATCCACCAGGGCCACTGGTATCCCA13850.10347999100433869No Hit
CCTCATCCATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAAGTTCAGTGGCAGTGGGTCTGGGACAGAGTT13720.10250869867000194No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13700.10235926908010397No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13650.10198569510535907No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA13530.1010891175659713No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13490.10079025838617536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)