FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338423
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT63740.4762321030048049No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57600.4303572189061306No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53000.3959884132295993No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA47450.3545217020329149No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT46850.350038814335976No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46320.34607893020368No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCTGTGCTCGTGGATGTGTCTGTGGT39400.29437629209898514No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32030.23931148822158615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29960.22384552566714708No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29710.22197765579342255No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG29330.21913849358536128No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29040.21697176453184083No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT28650.21405788752883056No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28480.2127877360146979No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGGCTGGTA28450.21256359162985095No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT28310.2115175845005652No Hit
GTCCCAGACCCACTGCCACTGAACTTGGCTGGGATACCAGTGGCCCTGGT28000.20920142585714682No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC26890.20090808361780993No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26300.19649991071582004No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26040.19455732604714654No Hit
CTGGTATCCCAGCCAAGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT25840.19306303014816692No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG24640.18409725475428917No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT24460.18275238844520753No Hit
GTGTATTACTGTGCGAGAGAAGTATTGTACCCCGACTACTGGGGCCAGGG24390.18222938488056467No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24190.18073508898158505No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23810.17789592677352378No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22950.1714704544079114No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22750.16997615850893177No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG22340.16691285191602356No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22280.16646456314632968No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22200.16586684478673783No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22130.16534384122209494No Hit
AAAGTGTACTGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAA21650.16175753106454385No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCAGTACACTTTTGGCCA21170.1581712209069928No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA20830.15563091787872743No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20830.15563091787872743No Hit
GTATTGTACCCCGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT20780.15525734390398252No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20360.15211932251612534No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20150.15055031182219672No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACTTGGCTGGGATACCA19790.1478605792040334No Hit
GTCAGAGTGTTAGCAGCAACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTG19610.14651571289495174No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGTCTTCTGGTTACACCTTTATCAA18870.14098681806872715No Hit
GGCCAAAAGTGTACTGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTG18810.14053852929903327No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACTGAGGCCAGTTATTATACTG18750.14009024052933938No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18650.1393430925798496No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC18230.13620507119199238No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18190.13590621201219646No Hit
CTGCTACTCTGGCTCCCAGATATCACTGGAGAAATGGTGATGACGCAGTC18070.13500963447280867No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17990.13441191611321684No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17950.1341130569334209No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATGGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGC17920.13388891254857396No Hit
TCCATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAAGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT17340.12955545444153305No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17280.12910716567183916No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17180.12836001772234937No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTA16690.12469899276984929No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16610.12410127441025744No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16410.12260697851127782No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16320.121934545356737No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16250.12141154179209414No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16240.12133682699714515No Hit
GTACAATACTTCTCTCGCACAGTAATACACGGCCGTGTCGTCAGATCTCA16160.1207391086375533No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15950.1191700979436247No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15910.11887123876382878No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15670.11707808368505324No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC15590.11648036532546138No Hit
GCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTA15540.11610679135071648No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAG15440.11535964340122666No Hit
GTGTTAGCAGCAACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATCCA15370.11483663983658379No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15290.11423892147699194No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAAGTT15150.1131929143477062No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT14820.11072732611438983No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGG14820.11072732611438983No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA14720.10998017816490004No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14720.10998017816490004No Hit
GTGCATCCACCAGGGCCACTGGTATCCCAGCCAAGTTCAGTGGCAGTGGG14710.10990546336995105No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACTGGAGAAATGGTGATGACGCAGTCTCCAGCCACC14650.10945717460025717No Hit
CTCCCAGGTTCAACTGGTGCAGTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGT14640.10938245980530818No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14590.10900888583056328No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACTGGAGAAATGGTGATGACGCAGTCTC14580.1089341710356143No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA14410.10766401952148164No Hit
GTATTACTGTGCGAGAGAAGTATTGTACCCCGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATC14270.1066180123921959No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14140.10564672005785915No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCACTTACAATGGT14120.10549729046796118No Hit
GGTTTCTGCTGGTACCAGCCTAAGTTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC14100.10534786087806321No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA14070.10512371649321628No Hit
GGAGAAATGGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG13920.10400299456898156No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC13830.10333056141444072No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13810.10318113182454278No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13800.10310641702959379No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13800.10310641702959379No Hit
GAGTGTTAGCAGCAACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATC13690.10228455428515498No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13590.10153740633566519No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13530.1010891175659713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATT200.00685814752.681250
TCGAAAC200.00793956850.7659842
GTATCAA27800.042.0226171
GTGGTAT2950.037.8791921
ATCAACG32850.035.0235523
GATTCAC1950.034.729951
AACGCAG33000.034.6657456
TCAACGC33050.034.6068344
CAACGCA33450.034.1955575
CGCAGAG34750.033.0173848
ACGCAGA34650.032.9173167
GTCCCGT850.003285364232.88021570
AGAGTAC35600.032.5166911
GCTTGTA1057.858034E-1032.2492371
GACAGCC2250.031.6042541
GAGTTTA4300.031.4992561
GCCGTAT450.00407901531.46123754
ATTCACT2050.031.36762
GACAGGT2050.029.7322251
TATAAGA803.2078133E-629.613492
Produced by FastQC (version 0.11.5)