FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650032
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC15510.23860363797474585No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14550.22383513427031285No Hit
GAGTATTACTGCCTGCTCTACTCTGATGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCCAAGTCAG14540.22368129569005835No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA13890.21368178797351514No Hit
GCCTTGGGCTGACTTGGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA13600.20922046914613435No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13180.2027592487754449No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT12930.19891328426908214No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12870.19799025278755505No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12800.1969133827257735No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12470.19183670957737467No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTGGGACGGTCAGCTTGGTCCCTC12460.19168287099712014No Hit
GTCCAGGAGTGTTTGTAGGTTGTTCCATGAATCAGTGCCCTGGGTGCTTG12120.1864523592684668No Hit
GTATTACTGCCTGCTCTACTCTGATGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCCAAGTCAGCC11780.18122184753981344No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11670.17952962315701382No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC11390.17522214290988752No Hit
GACTTGGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCATCAGAGTAGAGCAGGCAGTAATACTCAG11240.17291456420606985No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11160.17168385556403376No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11160.17168385556403376No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG10960.16860708395894355No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10640.16368424939079923No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10240.1575307061806188No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10180.15660767469909173No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10030.15430009599527408No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC9910.15245403303221994No Hit
GAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGA9850.15153100155069288No Hit
GTCTCTGGTGTCTCCATCACTAATAAATATTGGAGTTGGATCCGGCAGCC9490.14599281266153052No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9290.14291604105644032No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA9280.1427622024761858No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG9210.1416853324144042No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9010.138608560809314No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8970.13799320648829597No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8920.1372240135870234No Hit
GTTTGTAGGTTGTTCCATGAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGAGCGTAAC8860.13630098210549635No Hit
TCCCAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8680.13353188766091517No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCGGTGGTTA8600.13230117901887908No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8550.13153198611760653No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8230.12660915154946217No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGGTTCAGGGAGA8180.12583995864818964No Hit
CCTACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGG7890.12137863982080882No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7890.12137863982080882No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC7820.12030176975902725No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7800.11999409259851822No Hit
CCCAAGCACCATCAGAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGG7650.11768651389470056No Hit
GTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCCAAGTCAGCCC7570.1164558052526645No Hit
ACTCTGATGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCCA7550.11614812809215547No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCGGTGGTTACG7510.11553277377113742No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT7500.11537893519088291No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC7480.11507125803037388No Hit
GCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTCTGATGGTGCTTGGGTGTTCGGCGGAGGGACCAAG7440.11445590370935585No Hit
GCTCTACTCTGATGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCCAAGTCAGCCCAAGGCTGCCCC7410.1139943879685923No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7380.11353287222782879No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7330.11276367932655625No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC7300.11230216358579269No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7170.11030226204248406No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7150.10999458488197503No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC7150.10999458488197503No Hit
GCACTGGAGCAGTCACCGGTGGTTACGCTCCAAACTGGTTCCAGCAGAAA7140.10984074630172053No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6810.10476407315332169No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC6810.10476407315332169No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6770.10414871883230364No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6730.1035333645112856No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6710.10322568735077657No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT6700.10307184877052207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1850.073.08414570
CGCGTAT253.1040254E-668.95817639
TAGTACC200.004906803457.32946868
ATCGCGT408.442312E-751.58629637
ACGGACT200.007688367751.1775748
GGTATCA5400.049.9254531
CGTGAAA451.5463193E-647.25034757
CGAGACT2150.044.44692223
GTGGTAT1200.042.6578251
CGTATGC1550.042.383341
TATACAC2450.040.385033
CATCGCG603.015757E-740.06365636
TCTCGTA1300.039.73414238
TGGTATC1300.039.3673672
ACGAGAC2450.039.0014422
GTATCAA15750.038.3514141
TCGCGTA451.0120359E-438.26251238
GAGACTC2500.038.22435424
CTTATAC2500.038.2214131
CACGAGA2600.036.75135821
Produced by FastQC (version 0.11.5)