FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650032
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21900.3369064907573781No Hit
GAGTATTACTGCCTGCTCTACTCTGATGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCCAAGTCAG15510.23860363797474585No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC14780.22737342161616658No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14570.22414281143082188No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA13990.21522017377606026No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT13100.20152854013340882No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12900.19845176852831858No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12680.19506731976271938No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12190.18752922933024835No Hit
GTATTACTGCCTGCTCTACTCTGATGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCCAAGTCAGCC11820.18183720186083147No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC11680.17968346173726832No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTGGGACGGTCAGCTTGGTCCCTC11590.17829891451497773No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11360.174760627169124No Hit
GCCTTGGGCTGACTTGGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA11190.17214537130479732No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11070.17029930834174317No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10960.16860708395894355No Hit
GTCCAGGAGTGTTTGTAGGTTGTTCCATGAATCAGTGCCCTGGGTGCTTG10920.1679917296379255No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10830.1666071824156349No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG10360.15937676914367294No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10190.15676151327934626No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA9880.15199251729145644No Hit
GACTTGGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCATCAGAGTAGAGCAGGCAGTAATACTCAG9800.15076180864942035No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9500.14614665124178502No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9480.145838974081276No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9440.14522361976025794No Hit
GAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGA9140.14060846235262264No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC8990.138300883648805No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8950.13768552932778694No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG8510.13091663179658847No Hit
GTCTCTGGTGTCTCCATCACTAATAAATATTGGAGTTGGATCCGGCAGCC8480.13045511605582494No Hit
GTTTGTAGGTTGTTCCATGAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGAGCGTAAC8340.1283013759322618No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCGGTGGTTA8290.12753218303098926No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8070.12414773426539003No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7890.12137863982080882No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7840.12060944691953626No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7810.12014793117877275No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7780.1196864154380092No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7680.11814802963546411No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC7630.11737883673419154No Hit
GCTCTACTCTGATGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCCAAGTCAGCCCAAGGCTGCCCC7520.11568661235139192No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC7500.11537893519088291No Hit
GTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCCAAGTCAGCCC7460.11476358086986488No Hit
CCTACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGG7410.1139943879685923No Hit
TCCCAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7380.11353287222782879No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7310.11245600216604722No Hit
GCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTCTGATGGTGCTTGGGTGTTCGGCGGAGGGACCAAG7100.10922539198070248No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7060.10861003765968444No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGGTTCAGGGAGA7000.10768700617815738No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC6930.1066101361163758No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCGGTGGTTACG6880.10584094321510326No Hit
GCACTGGAGCAGTCACCGGTGGTTACGCTCCAAACTGGTTCCAGCAGAAA6870.10568710463484873No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC6830.10507175031383072No Hit
ACTCTGATGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCCA6620.10184114012848598No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6570.10107194722721344No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6570.10107194722721344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT150.00247061668.17161620
TACGTTA200.00706511952.28350447
CGAATAG200.007705718751.14839625
GCCGTAT305.650623E-447.04062354
GGTATCA6600.044.9382131
GTATCAA18400.039.834661
TATCAAC20550.035.313512
ATCAACG21100.034.3930173
ACGTAGG807.805647E-834.12257427
ATACTAT400.002759057334.07276
TGGTATC1900.034.0700762
TCAACGC21400.033.9108734
GTGGTAT1950.033.2169271
CAACGCA21850.033.212485
AACGCAG22150.032.7651676
CGCAGAG23000.031.5542838
ACGCAGA23150.031.3498257
TTTCGAT850.00443302330.9241470
AGAGTAC23700.030.622311
AATGGTA2151.3878889E-930.56455870
Produced by FastQC (version 0.11.5)