FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849142
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24270.2858179197354506No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24220.285229090069741No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18290.2153938917165798No Hit
ATAATACACAGCCGTGTCTTCAATTCTCAGGCTGTTCATTTGCAGATAGA18020.21221421152174785No Hit
GGGCTACAGTGGGTGGCAGTTGTATCATTTGATGGAAGAAATAAAGACTA17060.20090868194012312No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16950.19961325667556193No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCACGATATTAAGAACTGGTTAGCCTGGTATCAGCAGAAA15810.18618794029738253No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15330.18053517550657017No Hit
CAATAATACACAGCCGTGTCTTCAATTCTCAGGCTGTTCATTTGCAGATA14890.1753534744483255No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14790.17417581511690625No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14630.17229156018663544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14300.16840528439295194No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14090.16593219979697155No Hit
GGCTAACAGTTTCCCGCTCTCTTTCGGCGGAGGGGCCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14000.1648723063986942No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13900.16369464706727496No Hit
CTCCTGTGCAGCGTCTGGATTCACATTCAGTAGTTACTCTATGCACTGGG13850.16310581740156532No Hit
CTGTTAGCCTGTTGACAAAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC13650.16075049873872685No Hit
GTATTATTGTGCGAGAGATAAAGAGGTTGTCTCCAAGAACTGGTTCGATCCTTGGGGACAGGGAACCCAGGTCAC13580.15992613720673338No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13530.15933730754102376No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13470.1586307119421722No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13370.15745305261075296No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12640.14885613949139248No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12600.14838507575882479No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12480.1469718845611217No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12460.14673635269483787No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12370.14567645929656053No Hit
GTGTATTATTGTGCGAGAGATAAAGAGGTTGTCTCCAAGAACTGGTTCGA12200.1436744384331478No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12130.14285007690115434No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12070.14214348130230278No Hit
ATTAAGAACTGGTTAGCCTGGTATCAGCAGAAACCAGGTAAAGCCCCTAGACTCTTGATCTCTGGTGGATCCAC12040.141790183502877No Hit
GTAGTTACTCTATGCACTGGGTCCGACAGGCTCCAGGCAAGGGGCTACAG12010.14143688570345125No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11930.14049475823831586No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11480.13519529124692925No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11360.13378210004922614No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11230.13225114291838114No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGCCCCTCCGCCGAAAGAG11160.13142678138638766No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11140.13119124952010383No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10870.12801156932527186No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA10780.12695167592699452No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10680.1257740165955753No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10610.12494965506358183No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10340.12176997486874988No Hit
GTGGAGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACT10220.12035678367104678No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10200.12012125180476292No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10170.11976795400533714No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10160.11965018807219521No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10150.11953242213905331No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT10080.11870806060705982No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT9870.11623497601107942No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9860.11611721007793749No Hit
CTCTATGCACTGGGTCCGACAGGCTCCAGGCAAGGGGCTACAGTGGGTGG9770.11505731667966017No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9760.11493955074651827No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCTGGGTTCCCTGT9660.11376189141509901No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9610.11317306174938938No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9580.1128197639499636No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9580.1128197639499636No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9570.1127019980168217No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9330.10987561562141548No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9230.10869795628999626No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9200.10834465849057048No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8950.10540051016202237No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8910.10492944642945468No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8800.10363402116489351No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGCCCCTCCGCCGAAAGAGAGCGGGAAA8550.1006898728363454No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG8510.10021880910377769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTT350.006661250753.06537270
CTTATAC1400.048.8422741
GTGGTAT2500.046.4978451
ATCTCCG1500.045.57538210
TCGTATG1400.044.3974240
AATCTCG1400.041.8065336
TTATACA1650.041.4297262
TATACAC1700.040.2112053
ATCTCGT1550.040.0033137
ACGAGAC1650.039.38839322
CACGAGA1650.039.37911221
CCGTCTT1600.039.18814547
TCTCCGA1700.038.20289211
GTATCAA22400.038.1580281
GCCGTCT1550.038.145446
CGCTCAT1800.038.0534428
CGTATGC1550.037.91592441
GAGACGC1800.036.11454424
GAATCTC1650.035.44697635
CGAGACG1850.035.1322623
Produced by FastQC (version 0.11.5)