FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849142
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25000.29441483285481107No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22260.2621469671739238No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20080.23647399374898426No Hit
ATAATACACAGCCGTGTCTTCAATTCTCAGGCTGTTCATTTGCAGATAGA17860.21032995659147707No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16850.19843559734414265No Hit
GGGCTACAGTGGGTGGCAGTTGTATCATTTGATGGAAGAAATAAAGACTA15590.1835970897682602No Hit
CAATAATACACAGCCGTGTCTTCAATTCTCAGGCTGTTCATTTGCAGATA14860.17500017664889972No Hit
GTATTATTGTGCGAGAGATAAAGAGGTTGTCTCCAAGAACTGGTTCGATCCTTGGGGACAGGGAACCCAGGTCAC14320.1686408162592358No Hit
CTGTTAGCCTGTTGACAAAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC14170.16687432726210694No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13990.1647545404655523No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13800.1625169877358557No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13740.16181039213700418No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13590.16004390313987532No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13450.15839518007588835No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13360.15733528667761104No Hit
CTCCTGTGCAGCGTCTGGATTCACATTCAGTAGTTACTCTATGCACTGGG13320.15686422294504335No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCACGATATTAAGAACTGGTTAGCCTGGTATCAGCAGAAA13260.15615762734619182No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13130.1546266702153468No Hit
CTGTAGCCCCTTGCCTGGAGCCTGTCGGACCCAGTGCATAGAGTAACTAC13100.154273372415921No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12880.15168252188679868No Hit
GTGTATTATTGTGCGAGAGATAAAGAGGTTGTCTCCAAGAACTGGTTCGA12600.14838507575882479No Hit
GGCTAACAGTTTCCCGCTCTCTTTCGGCGGAGGGGCCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12470.14685411862797976No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12390.14591199116284437No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11790.1388460351743289No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11760.13849273737490314No Hit
GTAGTTACTCTATGCACTGGGTCCGACAGGCTCCAGGCAAGGGGCTACAG11760.13849273737490314No Hit
ATTAAGAACTGGTTAGCCTGGTATCAGCAGAAACCAGGTAAAGCCCCTAGACTCTTGATCTCTGGTGGATCCAC11620.1368440143109162No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11260.13260444071780692No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11060.1302491220549684No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGCCCCTCCGCCGAAAGAG11030.12989582425554264No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10900.12836486712469763No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10880.12812933525841377No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10850.12777603745898802No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10520.12388976166530451No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10480.12341869793273681No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10420.12271210233388526No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10370.12212327266817564No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10300.12129891113618217No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10220.12035678367104678No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10220.12035678367104678No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10160.11965018807219521No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10030.11811923094135021No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9990.11764816720878252No Hit
CTCTATGCACTGGGTCCGACAGGCTCCAGGCAAGGGGCTACAGTGGGTGG9720.11446848701395056No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9630.11340859361567324No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCTGGGTTCCCTGT9600.11305529581624746No Hit
GTGGAGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACT9550.11246646615053783No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9270.10916902002256394No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9210.1084624244237124No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9110.10728476509229316No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC8970.10563604202830622No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8790.10351625523175158No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8780.10339848929860965No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGCCCCTCCGCCGAAAGAGAGCGGGAAA8750.10304519149918387No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8690.10233859590033233No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8590.1011609365689131No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8580.10104317063577117No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACAGGCTAACAGTTTC8500.10010104317063576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG200.007708163551.1456534
GTATCAA22550.036.7757031
GTGGTAT2550.034.796411
TATCAAC26050.031.6812172
CTAGTAA450.00433342531.07338549
ATCAACG27150.030.394053
TCAACGC27350.029.9206734
CAACGCA27400.029.8660765
AACGCAG27650.029.842676
GTACGGA605.7927E-428.4259715
GACCTAT605.806689E-428.4142516
CGCAGAG29050.028.4044728
AGAGTAC30150.027.59434111
GCTTGCA500.00815475827.3017981
GCAGAGT30550.027.0098179
CAGAGTA30650.026.92169210
ACGGGTC1850.025.81537416
TTAACGG951.0799671E-525.33001735
CGATTCA8550.025.08564270
TCTGGAG10650.025.021470
Produced by FastQC (version 0.11.5)