FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035112
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36020.3479816676842699No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33470.32334665234293486No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25910.2503110774486239No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24580.23746222630980995No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22450.21688474290704773No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTCCTTAGCCTGGTACC22210.21456615322786327No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21810.21070183709588913No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21110.2039392838649344No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20890.20181390999234866No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19430.1877091561106431No Hit
GCACTATGGTGCCTCACCCCTCACTTTCGGCGGAGGGACCAAGGTGGCGATCAAACGAACTGTGGCTGCACCAT19130.1848109190116625No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19080.18432787949516574No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18770.1813330344928858No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACT18390.17766193416751036No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG17270.16684184899798282No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17080.16500629883529513No Hit
AAGTAATACCGGCCACTGGTAGACACTCTCGCACAGTAAAAGACGGCCGTGTCCTCAGATCTCAGGCTGCTCAA16680.161141982703321No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16130.15582854802185656No Hit
CCTCATATATGATACATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT15910.15370317414927082No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15640.15109476076018827No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15430.14906599479090185No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15160.14645758140181933No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT15060.1454915023688258No Hit
GTATTACTTCGATCGCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT14950.1444288154325329No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGC14710.14211022575334845No Hit
CCATAGTGCTGACAGAAATAGACTGCAAAGTCTTCAGGCTCCAGTCTGCT14550.14056449930055878No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14150.13670018316858465No Hit
GTGCAGCCACAGTTCGTTTGATCGCCACCTTGGTCCCTCCGCCGAAAGTG14120.1364103594586866No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT14100.13621714365208787No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGA13970.1349612409091963No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13840.1337053381663047No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13840.1337053381663047No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13530.13071049316402478No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13430.12974441413103122No Hit
CTATGGTGCCTCACCCCTCACTTTCGGCGGAGGGACCAAGGTGGCGATCAAACGAACTGTGGCTGCACCAT13260.12810207977494223No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13210.12761904025844548No Hit
GTCTATTTCTGTCAGCACTATGGTGCCTCACCCCTCACTTTCGGCGGAGGGACCAAGGTGGCGATCAAACGAA13170.12723260864524805No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACTTTGCAGTCTATTTCTGTCAGCACTATGGTGCCTCA13080.12636313751555386No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12930.12491401896606356No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12750.12317507670667521No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12590.12162935025388556No Hit
GTAAAAGACGGCCGTGTCCTCAGATCTCAGGCTGCTCAACTCCAAATAGA12470.12047005541429333No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12360.11940736847800046No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12150.11737860250871403No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12060.11650913137901985No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12050.1164125234757205No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCGCCACCTTGGTCC11710.11312785476354249No Hit
ATGGTGCCTCACCCCTCACTTTCGGCGGAGGGACCAAGGTGGCGATCAAA11620.11225838363384832No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11620.11225838363384832No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA11210.10829745959857484No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10950.10578565411279166No Hit
GTCTGGGCCTGAGGTGAAGAAGCCAGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCGCCTTCAGCAC10940.10568904620949231No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10940.10568904620949231No Hit
GAAATAGACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10930.10559243830619296No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10850.10481957507979814No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10810.10443314346660071No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10780.10414331975670266No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10480.10124508265772206No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10410.1005688273346266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)