FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101114
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC42440.38542784852431267No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT39480.35854598161498263No Hit
GAGTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGT38260.3474662932266777No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA37180.3376580444894898No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG35690.3241262939168878No Hit
GTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTG34510.31340987400033055No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33790.3068710415088719No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33550.3046914306783857No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33170.3012403801967825No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31370.2848932989681359No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC30830.2799891745995419No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT28260.256649175289752No Hit
GTAATATATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA27740.25192668515703187No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25780.23412653004139444No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC25350.23022139397010666No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGCCTGCATATGAGCTGCAGTAATACT25350.23022139397010666No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC25150.22840505161136815No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCGGCAGCGACGTT24770.22495400112976494No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24610.22350092724277415No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23770.21587228933607236No Hit
ATATATTACTGTGCGAGAGGAGAGGAGCGACGGGAGCAACTCCCTGACTA23280.21142225055716304No Hit
GCCCTCAGGGGTCCCTAATCGCTTCTTTGGCTCCAAGTCTGGCAACACGG21860.1985262198101196No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGCCTGCATATGAGCTG21820.19816295133837186No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGC21370.19407618103121022No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTC21000.19071594766754396No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20850.18935369089849008No Hit
CTCCTGCACTGGAACCGGCAGCGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGC20410.18535773770926534No Hit
GCAGTAATACTCAGCCTCATCCTCAGGCTGGAGACCAGAGACGGTCAGGG19890.1806352475765452No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19870.18045361334067136No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18520.1681933024191864No Hit
GTCAGTCACCATCTCCTGCACTGGAACCGGCAGCGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACGGCA18040.16383408075821396No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18020.16365244652234012No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17790.16156365280979082No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16780.1523911238981613No Hit
GTAATACTCAGCCTCATCCTCAGGCTGGAGACCAGAGACGGTCAGGGAGG16630.15102886712910743No Hit
TCTCTGGTCTCCAGCCTGAGGATGAGGCTGAGTATTACTGCAGCTCATAT15930.14467166887352265No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15900.14439921751971185No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15860.14403594904796416No Hit
GGATGAGGCTGAGTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTGGGACCACGGTCAC15700.14258287516097334No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15640.14203797245335179No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT15400.13985836162286558No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15360.13949509315111785No Hit
GGCTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGACGTCACTGAG14610.13268380930584844No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14610.13268380930584844No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14490.13159400389060533No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14390.13068583271123607No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14110.12814295340900214No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13700.1244194515735882No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13130.11924287585118344No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT12920.11733571637450801No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12740.11570100825164334No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA12720.11551937401576948No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12660.11497447130814793No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12520.11370303165703097No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12520.11370303165703097No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12330.11197750641622939No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12240.11116015235479704No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCGGCAGCGACGTTGGTGG12090.10979789558574317No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12030.10925299287812161No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12010.10907135864224775No Hit
CCTCAGGGGTCCCTAATCGCTTCTTTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGTCTCCAGC11980.108798907288437No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC11700.10625602798620307No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC11570.10507540545302302No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11550.10489377121714917No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11510.10453050274540149No Hit
CTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGCCCCCAAACTCATCA11400.1035315144480953No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11390.10344069733015837No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT11290.10253252615078912No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11230.10198762344316756No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11160.10135190361760908No Hit
GCCTCATCCTCAGGCTGGAGACCAGAGACGGTCAGGGAGGCCGTGTTGCC11140.10117026938173523No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11120.10098863514586137No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11110.10089781802792444No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT11050.1003529153203029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5350.079.3300870
TGGTATC3800.051.472822
TCGTATG5600.049.20203840
GTGGTAT4050.049.141561
ATCTCGT5100.048.31844337
TATCTCG5100.048.22509836
TCTCGTA5350.046.78222738
CGTATGC5850.046.60574741
GAGCCTA5750.046.0226231
ATGCCGT5900.045.9504144
TATACAC6000.045.526823
GCCTATC5400.045.34362433
GTATGCC6050.045.18264442
CCTATCT5600.044.99852434
CTTATAC6150.044.9770161
TTATACA6150.044.4164122
GCTTGAA6400.044.26895556
CCCACGA5950.044.2130719
CACGAGA6100.043.6807421
ATCTCCG6200.043.51823810
Produced by FastQC (version 0.11.5)