FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101114
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49370.4483641112546022No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC44000.3995953189224731No Hit
GAGTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGT37110.3370223246639313No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT36820.3343886282437604No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA35890.3259426362756263No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33340.30278427120171025No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33040.3000597576636025No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32480.2949739990591347No Hit
GTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTG31730.2881627152138652No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28860.2620982023659676No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC28310.25710326087943663No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG26020.23630614087188068No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25530.2318561020929713No Hit
GTAATATATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA25070.22767851466787273No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGCCTGCATATGAGCTGCAGTAATACT24980.22686116060644038No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23970.2176886316948109No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23250.21114979920335225No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGCCTGCATATGAGCTG23140.21015081090604604No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23010.20897018837286604No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21940.19925275675361495No Hit
ATATATTACTGTGCGAGAGGAGAGGAGCGACGGGAGCAACTCCCTGACTA21850.19843540269218266No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTC21470.19498435221057947No Hit
GCAGTAATACTCAGCCTCATCCTCAGGCTGGAGACCAGAGACGGTCAGGG20350.18481283500164378No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGC20090.18245158993528374No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCGGCAGCGACGTT19820.17999952775098674No Hit
GCCCTCAGGGGTCCCTAATCGCTTCTTTGGCTCCAAGTCTGGCAACACGG19640.17836481962812206No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19410.1762760259155728No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19060.1730974267877804No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC18800.1707361817214203No Hit
CTCCTGCACTGGAACCGGCAGCGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGC18020.16365244652234012No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17220.15638707708738603No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16740.15202785542641362No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16710.15175540407260285No Hit
GTAATACTCAGCCTCATCCTCAGGCTGGAGACCAGAGACGGTCAGGGAGG16480.14966661036005355No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15810.14358186345827953No Hit
GTCAGTCACCATCTCCTGCACTGGAACCGGCAGCGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACGGCA15720.14276450939684718No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15680.1424012409250995No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14570.13232054083410072No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14450.13123073541885763No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14300.12986847864980375No Hit
GGCTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGACGTCACTGAG14030.12741641646550675No Hit
GGATGAGGCTGAGTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTGGGACCACGGTCAC14020.12732559934756982No Hit
TCTCTGGTCTCCAGCCTGAGGATGAGGCTGAGTATTACTGCAGCTCATAT13730.12469190292739898No Hit
GTTTGGGGGCTTTGCCTGGGTGCCGTTGGTACCAGGAGACATAGTTATAG13710.12451026869152514No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13690.12432863445565127No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13640.12387454886596665No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13270.1205143155023004No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12650.114883654190211No Hit
CCTGAGGGCCGCTCAGTGACGTCATAAATGATGAGTTTGGGGGCTTTGCC12580.11424793436465251No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12580.11424793436465251No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12250.11125096947273397No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12250.11125096947273397No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12180.11061524964717549No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12100.1098887127036801No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12050.10943462711399546No Hit
CAGTAATACTCAGCCTCATCCTCAGGCTGGAGACCAGAGACGGTCAGGGA11960.10861727305256313No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11830.1074366505193831No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11830.1074366505193831No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11560.1049845883350861No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11380.10334988021222144No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT11270.10235089191491527No Hit
CTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGCCCCCAAACTCATCA11250.1021692576790414No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11210.1018059892072937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG9650.048.77161870
AATGGTA3600.046.14177770
GTGGTAT3350.041.2528151
GGTATCA10550.040.8951721
GCGCATA554.343803E-640.6674966
GTATCAA28000.039.0033151
TGGTATC3500.038.4885522
CCCATAA502.1339934E-433.706571
TCGTTGT9100.032.5375262
TTGTCGT9250.032.4705259
TATCAAC33100.032.4565472
CGTTGTC9050.032.4493363
TGTCGTT9100.032.32716860
TCAACGC33400.032.160644
AACGCAG33800.032.0832826
ATCAACG33450.032.01193
GTTGTCT9250.031.85337364
CAACGCA34100.031.5018835
GTCGTTG9400.031.3907761
CTTGTCG9500.031.12575558
Produced by FastQC (version 0.11.5)