FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length29-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCACCATCGGCAGCC10.9433962264150944No Hit
TTTATGATGTCAGTAAGCGGCCCTCAGGGATTTCTGATAGCTTCTCTGGATCCAAGTCTGGCAACACGGCCTCC10.9433962264150944No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTAAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10.9433962264150944No Hit
ATTAAATACATTCATAACCAAAAAAAAAAAAAAAAAAAAAAAAACGGACTATGTACACAAAAGCACACCCAACC10.9433962264150944No Hit
ACAGCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTAAAATATGGTACATCAACACTGCCTCTGTG10.9433962264150944No Hit
GTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATG10.9433962264150944No Hit
GGGTGAGGCCCTCACTTCATCCGGCGACTAGCACCGCGTCCGGCAGCGCCAGCCCTACACTCGCCCGCGCCATGG10.9433962264150944No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10.9433962264150944No Hit
ATCCAAATAGTTGTATCCATTTCCAGACAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGG10.9433962264150944No Hit
TGCCAGGCACGTAAGCGTCACCAGCTAGTTCAGGGCCAGCTCCTCCGACGGCGGCGGAATCACGTGCACCTAGGG10.9433962264150944No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGAAGGTGGGGGCACTTCTCCATATAACACTCTCCACTGTAGAA10.9433962264150944No Hit
CTGTAGGTGCTGTCCTTGCTGTCCTGCTC10.9433962264150944No Hit
TAAATAAACTGAAAATTTACACAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACTGTACTTAA10.9433962264150944No Hit
ACATAATCCATGGAAGGAGAGAAAGGGGATGAAACTGAAGGAACCAGGACTCTGGAGGATGCAGGATGCTGTTT10.9433962264150944No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTCACGGGCGAGCTCAGGCC10.9433962264150944No Hit
CAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGG10.9433962264150944No Hit
CCATCTATCCTGGTGACTCTACTGTTAGATACAGCCCGTCTTTCCAAGGC10.9433962264150944No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10.9433962264150944No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10.9433962264150944No Hit
GGGCAGGGCACAGTCACCTCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGG10.9433962264150944No Hit
CCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCCGC10.9433962264150944No Hit
CGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTACGAGGAGTTTGTAG10.9433962264150944No Hit
ATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGC10.9433962264150944No Hit
AGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTG10.9433962264150944No Hit
CTTTGGACTCTGACCCTTTTTCCACAGGGGACATACAACTATTGCGTTCAACCACAACATCTATCACCAAA10.9433962264150944No Hit
GCCCTGGACCGCCTTCCTCTTGCCCCGGTGGATGATGAACTTCCTCTTGAAATGGGACAGGAACTTGGGGTTCTC10.9433962264150944No Hit
GGCACTGCAGCTGGCTCCATCTTGCTAATGTGTGTGATGAATCGAAGACGAAGTTTTAAAGCTGGTTTTAAGTTA10.9433962264150944No Hit
GAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTT10.9433962264150944No Hit
CTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGAGTTCTATCCGGGCCTC10.9433962264150944No Hit
ATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTG10.9433962264150944No Hit
TCCACGGATTACCCTCAAATCATTCTAAGGTATTCAATATTAACCCCATAGGCACCACATAATCGCACACCAAT10.9433962264150944No Hit
ATACACGGCCGTGTCTTCCATTTACAGGCTTTTCATTTGCAGATACAATGACTTAATTTAATAATCAATAGAGAT10.9433962264150944No Hit
CCCCACCAGGGAACTTCCAATGTATCCACACAGACCTACTCACCTGTTTT10.9433962264150944No Hit
GCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCCAAGTCACC10.9433962264150944No Hit
CCCCAAGTGCTGGGATTATAGACGTGACATTTATTAACTCCTTTCTTGCC10.9433962264150944No Hit
GAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGAC10.9433962264150944No Hit
TGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATG10.9433962264150944No Hit
GTGTGAGAGGTGGGGAGGTGTTCGACCCCTGGGGCCAGGGAACCCTGGTC10.9433962264150944No Hit
TGAGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGT10.9433962264150944No Hit
AGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCA10.9433962264150944No Hit
CCTGTCTGGAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAG10.9433962264150944No Hit
GACCCAGTCTCCAGCCTCCCTCGCTCTGTCTCAGGGCGAGAGGGCCACCATCAACAGCAAGTCAAGCAGGACTAT10.9433962264150944No Hit
ACAGGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCG10.9433962264150944No Hit
GGCTGCTTTTAGGAACTCGGAGTGAGCCTCACTGAATGAATCATCGAAAA10.9433962264150944No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACA10.9433962264150944No Hit
CTACAAACTCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG10.9433962264150944No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9433962264150944No Hit
CCACTACACCCCGCACAGTAATACACACCATTGTCCTCGGCTATCAGGATGTCCATATGCAGATAAAGCGAGCA10.9433962264150944No Hit
AAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGA10.9433962264150944No Hit
GTACGGGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGCCTGAGAGATGC10.9433962264150944No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10.9433962264150944No Hit
TACCAGGCCAACCAACTACTAATACTTTGACTGGCCCGGCAAGTGATGGT10.9433962264150944No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10.9433962264150944No Hit
CCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTT10.9433962264150944No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC10.9433962264150944No Hit
CAAACAGCTATCACCAGGCTCGGTAGGTTTGTCCCCTCAACAAATAAATATTCCCACTATATTGCTACAAAGA10.9433962264150944No Hit
GGCTGTAGGTGCTGTCCTTGCTGTTCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10.9433962264150944No Hit
CCTCTGAGCAGTAGTAACCCAGGTTGAACTCTTCAGGTATTTTCTGGAAG10.9433962264150944No Hit
TACCGTCGTAGTACTGCACGTTTACCAGCCCCCTAAGTCTCGCACAGTAATAGATGCCGGTGTCCGAGGCCTTCA10.9433962264150944No Hit
GCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAA10.9433962264150944No Hit
GTTTATTACTGTCAGCACCGTAGTGCCCTGGGGTTCACTTTTGGCCAGGGGACCAAGCTAGAGTTCAGACGAA10.9433962264150944No Hit
GCCCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCC10.9433962264150944No Hit
GGGGCACTTCTCCCTCTAACACTCTCCCCTGT10.9433962264150944No Hit
AGGGACCAATGTGGAAATTAAACGAACTGTGGCTGCACCATCTGTCTTCA10.9433962264150944No Hit
GGCATTGTCTCTGGAGATGGTGAGTCGGCCCTTCACTGAGTCTGCGTGAAATATGTAATCACTACTGCCACTAAT10.9433962264150944No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGAAAGATGGTGCAGCCACAGTT10.9433962264150944No Hit
AAGCTCTACAAACTCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT10.9433962264150944No Hit
GTGAACTGATAAGATGAGCACATGAATGATAAGCCGTAGATTCTCTGCACACACTAAGTTGGACCTTCCCGTCCA10.9433962264150944No Hit
CTCTGCGAGAGGAGCCAACCACTAGAATTCCGCCGTGATACCATTAGGTGAACAGCACTCAACACAGAGCGCTCA10.9433962264150944No Hit
TGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCGCTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCAT10.9433962264150944No Hit
CCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTCTGTT10.9433962264150944No Hit
ATCTTGGGGGGTGCTGTCGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCG10.9433962264150944No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATAACGATAACAGTCGGCCCTCTGG10.9433962264150944No Hit
CATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTATGTCCC10.9433962264150944No Hit
ATAAAACACATCTCCTAGACAACCAGACTAGATCACATGGAGTATGACGTGTTCTTCTTTAAAAAAAAAATTGAG10.9433962264150944No Hit
CATTAGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAGACTCCTGATC10.9433962264150944No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10.9433962264150944No Hit
CTCTGGCTCTCCAAGGCAAAGTCAAAAAAAAAAAAAAAACAGGGGGGGATTACTAGGAACATTTTTTTCAACA10.9433962264150944No Hit
CTAACAACCTGAGAGAAGATTACAAATTTCACCAAACTTTCAGCACAGAA10.9433962264150944No Hit
CTCCCATCCTTTGGCCTCTGACCCTTTTTCAACAGGGGACCTACCCAAATTGCGGACCTCCAGCTCATCTATAA10.9433962264150944No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10.9433962264150944No Hit
AGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC10.9433962264150944No Hit
TACGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCA10.9433962264150944No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGT10.9433962264150944No Hit
CAGGTATCAACGAAGAGTACGGGGAGCTACACCAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGT10.9433962264150944No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10.9433962264150944No Hit
GTGGTCCACCATGCAGGAGAAGGTGTCACCCTTCTTCCAGTCCTCGGCTG10.9433962264150944No Hit
CTATAAGCCTGTTGACAATAATAAGTCGCGAAATCCTCAGGCTGAAGGTTCTTGATGGTGAGAGTGAAATCTGTC10.9433962264150944No Hit
CTAAGAGCACCATGCGCGCAGCAGCCATCTCCACTCCAAAGTTAGACAAA10.9433962264150944No Hit
CTATTTCCCCACTGCAACCAGAGACCCAGAGCATTAGCAGCCCCAGGAGC10.9433962264150944No Hit
ACAGGCCAAGAGCTCGCTGTATCTGCAAATGGACAGCCTGAGAGCCGAGG10.9433962264150944No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT10.9433962264150944No Hit
GGGTAGGTCCCCTGTGGAAGAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10.9433962264150944No Hit
GGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGGTGGGGCACTTCTCCCTCTAACA10.9433962264150944No Hit
CTTTAATGAGGGCATTGATATTATCCTCCGTGACTGTCACCTCATCGTCG10.9433962264150944No Hit
GATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATTCATAGGGACAGCAGCCGG10.9433962264150944No Hit
GGTAGACGCCTTACCCGTACTCTGCGTTGATAC10.9433962264150944No Hit
GTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCATCCATGGAACCATGGGGAGACGTTCCCCTGCACTG10.9433962264150944No Hit
GGTAAACACACCCATGTCAATGTGTCTGTTGTCATGGCGGAGGTGGACGTCACATGCTACTGA10.9433962264150944No Hit
ACTCTGCGTTGATACCACTGCTACGCAGAGTACTTTTTTTTTTTTTTTTT10.9433962264150944No Hit
CTATAAACCAAGAGAACAAGAAAGAGAACAAAAGAAAAGAGAATAAACCA10.9433962264150944No Hit
CAAGTGGGAAGAGCGCTGTTCAAGGACAACCTGAGCGTGACCTCTGTGGCTGCAACAGCGTGTCCAGTGTCCTGC10.9433962264150944No Hit
CGTGCACACTGACTGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGAG10.9433962264150944No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGTGGGGGTCAGGCTGGAACTGAGGAGCAGGTG10.9433962264150944No Hit
GAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAG10.9433962264150944No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10.9433962264150944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTGG50.0139.070
Produced by FastQC (version 0.11.5)