FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815173
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT36340.44579494168722467No Hit
GTTATTATACTGCTGACAGTAATAAATCGCAAAATCTTCAGACTGCAGGCTGCTGATGAGGAGAGTGAACT28420.3486376511488972No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27770.34066388361734257No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26070.31980941468866114No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25720.3155158475562856No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17520.2149237033120577No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17440.21394231653894327No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTGGCAGCAACTTAGCCTGGTACCAGCAGCGACCTGGCCAGGCTCCCAGGCT16210.19885349490230908No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15690.19247448087706537No Hit
GAGTTCACTCTCCTCATCAGCAGCCTGCAGTCTGAAGATTTTGCGATTTATTACTGTCAGCAGTATAATAACTGG15610.19149309410395093No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15290.18756754701149328No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT15220.18670883358501814No Hit
GTCTGGGACAGAGTTCACTCTCCTCATCAGCAGCCTGCAGTCTGAAGATT15130.18560477346526444No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTGGCAGCAACTTAGCCTGGTA15030.1843780399988714No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14810.18167922637280676No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14800.18155655302616744No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14790.18143387967952815No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14610.1792257594400207No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14460.17738565924043118No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTACTCAGCTCCATGTAGG14360.17615892577403816No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14280.17517753900092373No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTGGCAG14280.17517753900092373No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT13810.16941189170887652No Hit
GGCCAAAAGTGGGCCAGTTATTATACTGCTGACAGTAATAAATCGCAAAA13490.16548634461641884No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13280.1629102043369935No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13230.16229683760379698No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12650.15518178349871745No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12150.14904811616675234No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG12050.14782138270035933No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11880.14573593580749117No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11870.14561326246085188No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT11740.14401850895454094No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA11470.14070632859527976No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGCGGCACCTTCAGCA11260.13813018831585444No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11100.13616741476962557No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10800.13248721437044653No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10790.1323645410238072No Hit
GTGTATTACTGTGCGAGACATGGCACCTCGGGCAAGGTGTGGTTCGGCCC10580.12978840074438186No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10480.12856166727798884No Hit
GTCAGAGTCTTGGCAGCAACTTAGCCTGGTACCAGCAGCGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG10470.12843899393134955No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGACAAATCCTCCCTATATTTGAT10370.1272122604649565No Hit
GGATACCAGTGGCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCGCTGCTGGTACCAGG9970.12230532659938444No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9840.1207105730930735No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9680.11874779954684465No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9670.11862512620020535No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9570.11739839273381233No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9470.1161716592674193No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9250.11347284564135465No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9090.11151007209512583No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8950.10979264524217558No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG8840.10844323842914326No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGGCTACTCAGCTCCATGTA8820.10819789173586467No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8800.10795254504258604No Hit
GCCTACATGGAGCTGAGTAGCCTGAGATCTGACGACACGGCCGTGTATTA8780.10770719834930745No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC8760.10746185165602885No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8710.10684848492283233No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8610.10562175145643932No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8540.1047630380299642No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8510.10439501799004629No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8490.10414967129676767No Hit
GCTCATCACCATGGACTGGACCTGGAGGCTCCTCTTTGTGGCGGCAGCAG8480.10402699795012837No Hit
TCTATGATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT8290.10169620436398163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2550.051.37367640
GCGAATC200.00779283151.00432611
TAGCGCT2550.049.4740829
CGCTCAT2450.048.83314532
CGTATGC2700.047.33105541
CTAGCGC2750.047.0926828
CACGAGA2700.046.6194221
GCGCTCA2650.046.39750331
GCCGTCT2650.045.9321946
GAGACTA2850.044.18203724
ACGAGAC2850.044.1711922
TCTCCGA2900.043.38298811
ATCTCGT2550.043.05586637
ATGCCGT3000.042.7551244
ACTAGCG2950.042.72368227
CGAGACT2950.042.679123
AGACTAG3000.041.988425
ATCTCCG3050.041.24939710
CATCTCG2700.040.6211336
CCGTCTT3050.039.95340747
Produced by FastQC (version 0.11.5)