FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815173
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT33310.40862491765551606No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27720.34005051688414606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27620.33882378341775304No Hit
GTTATTATACTGCTGACAGTAATAAATCGCAAAATCTTCAGACTGCAGGCTGCTGATGAGGAGAGTGAACT27530.3377197232979993No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23670.2903678114952286No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22930.28128998384392023No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16950.20793132255361746No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16090.19738141474263746No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15810.193946561036737No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15720.19284250091698327No Hit
GAGTTCACTCTCCTCATCAGCAGCCTGCAGTCTGAAGATTTTGCGATTTATTACTGTCAGCAGTATAATAACTGG14940.18327397987911767No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14930.18315130653247838No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT14810.18167922637280676No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTGGCAGCAACTTAGCCTGGTACCAGCAGCGACCTGGCCAGGCTCCCAGGCT14730.18069783959969232No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT13890.17039327848199093No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13740.1685531782824014No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13650.16744911816264768No Hit
GTCTGGGACAGAGTTCACTCTCCTCATCAGCAGCCTGCAGTCTGAAGATT13650.16744911816264768No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13420.16462763118994375No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTGGCAGCAACTTAGCCTGGTA13390.16425961115002582No Hit
GGCCAAAAGTGGGCCAGTTATTATACTGCTGACAGTAATAAATCGCAAAA13200.16192881756387908No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12890.1581259438180607No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12610.15469109011216023No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTGGCAG12400.15211494983273488No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12220.14990682959322743No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12200.14966148289994885No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12170.14929346286003095No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTACTCAGCTCCATGTAGG11920.14622662919404839No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11670.14315979552806582No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA11420.14009296186208328No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11190.1372714748893793No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11190.1372714748893793No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG11050.1355540480364291No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11040.13543137468978977No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGCGGCACCTTCAGCA10470.12843899393134955No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10250.1257401803052849No Hit
GTGTATTACTGTGCGAGACATGGCACCTCGGGCAAGGTGTGGTTCGGCCC10190.12500414022544906No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9350.11469957910774768No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9350.11469957910774768No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9220.11310482560143674No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9120.11187809213504374No Hit
GGATACCAGTGGCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCGCTGCTGGTACCAGG9120.11187809213504374No Hit
GTCAGAGTCTTGGCAGCAACTTAGCCTGGTACCAGCAGCGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG9100.11163274544176512No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9090.11151007209512583No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9050.11101937870856861No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGACAAATCCTCCCTATATTTGAT9020.11065135866865071No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8960.1099153185888149No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8840.10844323842914326No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC8820.10819789173586467No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8720.10697115826947164No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8530.10464036468332488No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8500.10427234464340698No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8450.10365897791021048No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8270.10145085767070304No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA8240.10108283763078513No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGGCTACTCAGCTCCATGTA8170.10022412420431001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8200.038.1042061
GTCGGTT400.002819737233.9228612
GTATCAA24000.033.6794241
TATCAAC23900.033.494992
ATCAACG24350.032.8739743
AACGCAG24750.032.4837046
TCAACGC24650.032.336284
CAACGCA24750.032.2076035
CGCGGTA7700.031.8168370
ACGCAGA26000.030.921997
CGCAGAG26250.030.6274958
CGGGATT450.005022444830.1499484
AGAGTAC26700.029.73014511
GACAGGT1950.029.6082861
GTACATG1501.8189894E-1229.4341181
GCACGAA500.00606671229.00670264
CGCGGCA500.00762511627.67996442
ACTTCCA8600.027.67324370
GAGCACG500.00794463427.44811237
GTGTTAT500.0083494227.1699541
Produced by FastQC (version 0.11.5)