FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475696
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAACTTTGTACACTTTTGGCCCGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT19070.4008862803134775No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18500.38890383774511456No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16970.3567404392721402No Hit
GTTTTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGCTGATGATGAGAGTGAATTCTGTC15860.3334062090074333No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12670.2663465742827352No Hit
ATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA12320.25898893410917895No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11990.25205173051696883No Hit
CTTTTGGCCCGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11800.24805758299418115No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10910.22934815512428106No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10640.2236722612761091No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10610.22304160640409No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9850.20706501631293936No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA9820.20643436144092026No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9780.2055934882782281No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9350.19655410177928762No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9170.19277017254717296No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9120.1917190810938078No Hit
GACTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGT8900.18709427869900105No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAA8440.17742423732804144No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8200.1723789983518886No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8090.17006659715448522No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8060.1694359422824661No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8020.16859506911977398No Hit
GGCCAAAAGTGTACAAAGTTTTATACTGTTGGCAGTAATAAGTTGCAGAA7980.16775419595708183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7750.16291917527160202No Hit
GAATTCACTCTCATCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTA7440.15640240826073795No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7260.15261847902862333No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7060.14841411321516262No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7040.14799367663381655No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT6800.1429484376576637No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6720.14126669133227945No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTGCCACC6500.13664188893747267No Hit
CCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCATCATCAGCAGCCTGCAGCCTGATGATTCTG6270.13180686825199286No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6260.13159664996131984No Hit
ACCTTGAGGGGACCCCACTTTCTAAAGTAGACGTCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG6220.1307557767986277No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6180.12991490363593555No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6150.12928424876391645No Hit
ACTTATTACTGCCAACAGTATAAAACTTTGTACACTTTTGGCCCGGGGAC6110.1284433756012243No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6100.12823315731055127No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC5860.12318791833439845No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5560.11688136961420739No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5490.11540984157949614No Hit
CTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGA5480.11519962328882312No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC5460.11477918670747705No Hit
ACTTTAGAAAGTGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCATCATCAGCAGC5410.11372809525411187No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5410.11372809525411187No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5410.11372809525411187No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5400.11351787696343883No Hit
GTTACTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGTGGCAGGAGA5380.11309744038209277No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC5370.11288722209141973No Hit
CATCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTATTACTGCCAACAGTATAAAACTTTGTACACTTTTGG5170.10868285627795903No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT5130.1078419831152669No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGTCTACTTTAGAAAGTGGGGTCCCCT5050.10616023678988262No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCGGGCCAAAAGTG5020.1055295819178635No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC4990.1048989270458444No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4850.10195587097642192No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT4770.10027412465103763No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC4770.10027412465103763No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4760.10006390636036461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)