FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522126
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17420.33363594228213117No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16710.3200376920513439No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT16250.31122755809900293No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12820.24553460275872108No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12520.23978886322458562No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12010.2300211060165554No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCAT11910.2281058595051769No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11080.21220931346073554No Hit
TACTTAGCCTGGTATCAACAAAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTCTCCAAAAGGGCCG10570.2024415562527053No Hit
GTACATACTTAGCCTGGTATCAACAAAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTCTCCAAAAGGG10470.20052630974132682No Hit
GCTCAGTGAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT9910.1898009292776073No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9640.1846297636968854No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9630.18443823904574758No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9610.18405518974347188No Hit
GTGAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTCA9480.18156536927867986No Hit
TCTATGATGTCTCCAAAAGGGCCGCTGGCGTCCCAGACAGGTTCAGTGGCCGTGGGTCTGTGACAGACTTCACT9320.17850097486047428No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9280.1777348762559229No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9160.17543658044226873No Hit
GTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCATGCAGGGCCAGTCAGA8810.16873321765244403No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8230.15762478788644885No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8180.1566671646307596No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8090.154943442770519No Hit
GTCTAGGCCAGTTGCTACGCTGCTGACAATAATAAACTGCAAAATCTTCG7760.14862312928297No Hit
GGCCTAGACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACT7720.14785703067841863No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7570.1449841609113509No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGCGC7520.14402653765566167No Hit
CTCTCATGCAGGGCCAGTCAGAGTGTCAGTACATACTTAGCCTGGTATCAACAAAGACCTGGCCAGGCTCCCAGG7320.1401960446329047No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7290.13962147067949116No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC7040.13483335440104494No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTCTAGGCCAGTTGCTACGCTG6990.13387573114535573No Hit
GTGACAGACTTCACTCTCACCATCAGCCACTTGGAGCCCGAAGATTTTGC6870.13157743533170155No Hit
GTCCCAGACAGGTTCAGTGGCCGTGGGTCTGTGACAGACTTCACTCTCAC6670.1277469423089446No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6560.12564017114642825No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6540.12525712184415255No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6520.12487407254187687No Hit
GCGTAGCAACTGGCCTAGACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT6400.1225757767282227No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6370.12200120277480915No Hit
CATCTATGATGTCTCCAAAAGGGCCGCTGGCGTCCCAGACAGGTTCAGTGGCCGTGGGTCTGTGACAGACTTCA6180.11836223440319002No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5990.11472326603157092No Hit
GTCTGGGACGCCAGCGGCCCTTTTGGAGACATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTTTGTTGATA5960.11414869207815738No Hit
CTCCAAAAGGGCCGCTGGCGTCCCAGACAGGTTCAGTGGCCGTGGGTCTGTGACAGACTTCACTCTCACCATCAG5850.11204192091564107No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT5780.11070124835767611No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5740.10993514975312473No Hit
GTCTGTGACAGACTTCACTCTCACCATCAGCCACTTGGAGCCCGAAGATT5590.10706227998605701No Hit
CTGCTACTCTGGCTCCCAGATGTCACCGGACAAATTGTGTTGACACAGTC5510.10553008277695422No Hit
CTCATGCAGGGCCAGTCAGAGTGTCAGTACATACTTAGCCTGGTATCAACAAAGACCTGGCCAGGCTCCCAGGCT5470.10476398417240283No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5430.10399788556785143No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT5370.10284873766102434No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5370.10284873766102434No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5350.10246568835874864No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5320.1018911144053351No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5270.10093349114964588No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGG503.5452005E-451.69273470
TCGTATG2750.040.16192640
TGACGCA400.001332329439.51609868
CTTATAC2750.039.291941
ACGAGAC2750.039.27311722
CCGTCTT2950.039.15376747
CGTATGC2800.038.26083841
ACGCGAC1100.037.98299845
ATGTCGT1552.2719178E-937.51892570
CCCACGA2900.037.22748619
TTATACA2900.037.2274862
ATGCCGT3000.037.08395444
TATACAC2950.036.596513
ATCTCGT2850.036.08323337
GTATGCC3000.035.7697642
TCTACAT2950.035.7369332
GCACCGA853.6852725E-935.7219627
CACGAGA3050.035.41018721
ATCTCCG3050.035.3966210
AAAACCG450.002375796235.1254268
Produced by FastQC (version 0.11.5)