FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627471
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21720.3461514556051196No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19470.3102932247067992No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17340.27634743278972257No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14780.2355487345231891No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13620.21706182437116617No Hit
GTCTCTGGCGGCTCCATCAGCAGTGGTGGGCACTTCTGGAGCTGGATCCG13120.20909332861598384No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG12360.19698121506810676No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11980.19092515829416817No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11870.18917208922802806No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11750.18725965024678431No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11750.18725965024678431No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC11580.1845503616900223No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11230.1789724146613947No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11160.17785682525566918No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT10910.17387257737807804No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA10720.17084454899110874No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10620.1692508498400723No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10430.16622282145310302No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10360.16510723204737748No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT10230.1630354231510301No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10200.16255731340571916No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9980.15905117527343893No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9690.15442944773543318No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT9680.15427007782032953No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT9650.1537919680750186No Hit
GAAATACACGGCCGTGTCCGCGGCAGTCAGAGAGCCCAGCTTCAGGGAGA9610.15315448841460402No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9420.15012646002763474No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT9350.14901087062190924No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8740.1392893058005868No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8730.13912993588548317No Hit
CACCTGCACTGTCTCTGGCGGCTCCATCAGCAGTGGTGGGCACTTCTGGA8500.13546442783809928No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG8260.13163954987561177No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8250.13148017996050815No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8210.13084270030009354No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8080.12877089140374615No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8010.1276553019980206No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCACCGTAGTGCCCTGGGGTTCACTTTTGGCCAGGGGA7820.12462727361105136No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7770.12383042403553311No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA7750.12351168420532581No Hit
GTTTATTACTGTCAGCACCGTAGTGCCCTGGGGTTCACTTTTGGCCAGGGGACCAAGCTAGAGATCAGACGAA7710.12287420454491124No Hit
CTTCTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACGTCTCTTACAGTGGGAGCAC7630.12159924522408207No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7510.11968680624283833No Hit
GTAGTGCCCTGGGGTTCACTTTTGGCCAGGGGACCAAGCTAGAGATCAGA7500.11952743632773466No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA7450.11873058675221644No Hit
CTATGATACTAGTGGTTTTTCACCACAAGGGTACTGGGGCCAGGGAACCC7450.11873058675221644No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7390.11777436726159456No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7360.11729625751628363No Hit
GGGCACTACGGTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT7360.11729625751628363No Hit
GGCCAAAAGTGAACCCCAGGGCACTACGGTGCTGACAGTAATAAACTGCA7290.1161806681105581No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7250.11554318845014351No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7200.11474633887462529No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7190.11458696895952163No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7170.11426822912931434No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTGACCATATCAGTAGACGCGTCT7120.11347137955379612No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC7080.11283389989338152No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7040.11219642023296693No Hit
GTGCAGCCACAGTTCGTCTGATCTCTAGCTTGGTCCCCTGGCCAAAAGTG7040.11219642023296693No Hit
CTTTTGGCCAGGGGACCAAGCTAGAGATCAGACGAACTGTGGCTGCACCA7030.11203705031786329No Hit
CCTTGGGGCTGGTCGGGGATGCCGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCTTGTGGTGAAAAAC7030.11203705031786329No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6970.1110808308272414No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG6940.11060272108193048No Hit
GTATCATAGTTATGCGTCCTCGCACAGAAATACACGGCCGTGTCCGCGGC6920.11028398125172319No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6890.10980587150641226No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG6770.10789343252516849No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6750.1075746926949612No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCTAGCTTGGTCC6730.1072559528647539No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC6710.10693721303454663No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT6690.10661847320433933No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6660.10614036345902839No Hit
GGGTTCCCTGGCCCCAGTACCCTTGTGGTGAAAAACCACTAGTATCATAGTTATGCGTCCTCGCACAGAAATACA6580.10486540413819923No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT6450.10279359524185182No Hit
CTACGGTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCT6430.10247485541164453No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6400.1019967456663336No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6320.10072178634550441No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)