FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627471
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22420.3573073496623748No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21560.34360153696346124No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16470.2624822501757053No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15460.2463858887502371No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14000.22311788114510472No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12760.2033560116722526No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG12020.19156263795458275No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC11860.18901271931292443No Hit
GTCTCTGGCGGCTCCATCAGCAGTGGTGGGCACTTCTGGAGCTGGATCCG11820.18837523965250985No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11460.18263792270877857No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11420.182000443048364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10710.1706851790760051No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT10610.16909147992496865No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT10540.1679758905192431No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA10380.1654259718775848No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10290.16399164264165197No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9740.15522629731095142No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9460.15076393968804933No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9340.14885150070680558No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9270.14773591130108005No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT9200.14662032189535454No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9190.1464609519802509No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9180.14630158206514723No Hit
GAAATACACGGCCGTGTCCGCGGCAGTCAGAGAGCCCAGCTTCAGGGAGA8940.14247670410265972No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT8910.1419985943573488No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8830.1407236350365196No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8800.14024552529120868No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8770.13976741554589772No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8580.13673938715892847No Hit
CACCTGCACTGTCTCTGGCGGCTCCATCAGCAGTGGTGGGCACTTCTGGA8450.13466757826258105No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8230.13116144013030084No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA7980.12717719225270968No Hit
GTTTATTACTGTCAGCACCGTAGTGCCCTGGGGTTCACTTTTGGCCAGGGGACCAAGCTAGAGATCAGACGAA7960.1268584524225024No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7890.12574286301677687No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7890.12574286301677687No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7860.1252647532714659No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7860.1252647532714659No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCACCGTAGTGCCCTGGGGTTCACTTTTGGCCAGGGGA7840.12494601344125864No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7770.12383042403553311No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG7760.12367105412042947No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7560.12048365581835654No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7390.11777436726159456No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7370.11745562743138727No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7310.1164994079407654No Hit
GGGCACTACGGTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT7290.1161806681105581No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA7190.11458696895952163No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTGACCATATCAGTAGACGCGTCT7170.11426822912931434No Hit
CTTCTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACGTCTCTTACAGTGGGAGCAC7100.11315263972358881No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6940.11060272108193048No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC6890.10980587150641226No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6850.10916839184599766No Hit
GGGTTCCCTGGCCCCAGTACCCTTGTGGTGAAAAACCACTAGTATCATAGTTATGCGTCCTCGCACAGAAATACA6830.10884965201579037No Hit
GGCCAAAAGTGAACCCCAGGGCACTACGGTGCTGACAGTAATAAACTGCA6740.10741532277985756No Hit
GTAGTGCCCTGGGGTTCACTTTTGGCCAGGGGACCAAGCTAGAGATCAGA6720.10709658294965026No Hit
GTATCATAGTTATGCGTCCTCGCACAGAAATACACGGCCGTGTCCGCGGC6630.10566225371371744No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6630.10566225371371744No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC6620.10550288379861379No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6610.10534351388351015No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6600.10518414396840652No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCTAGCTTGGTCC6600.10518414396840652No Hit
CTTTTGGCCAGGGGACCAAGCTAGAGATCAGACGAACTGTGGCTGCACCA6580.10486540413819923No Hit
CCTTGGGGCTGGTCGGGGATGCCGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCTTGTGGTGAAAAAC6580.10486540413819923No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6470.10311233507205911No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6420.10231548549654086No Hit
CTCTTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTGACCATATCAGTAGACGCGTCTAAGAA6400.1019967456663336No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC6400.1019967456663336No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCC6360.101359266005919No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6340.10104052617571171No Hit
GTGCAGCCACAGTTCGTCTGATCTCTAGCTTGGTCCCCTGGCCAAAAGTG6340.10104052617571171No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6340.10104052617571171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTCG200.006475078453.44887558
ATGCATG606.097447E-446.31228370
GGTATCA5250.045.9210321
GTATCAA13950.040.1626431
CATGCAT500.002315776235.30969269
AACGGCC501.9249503E-434.3004237
TATCAAC16350.034.2371752
GACAGGT906.0517777E-933.955691
GGTACCT502.0445771E-433.95027519
ATCAACG16900.032.9195753
TCAACGC16950.032.8224684
GAGTTTA1350.032.6980741
AATGAAC450.003681009632.12515663
CAACGCA17350.032.0657545
AACGCAG17400.031.9761586
TACTGTG652.6017453E-531.3162367
CGAGCTT450.00434003231.06240849
ACGCAGA18250.030.4868587
CGGGTAC450.005002670530.1732117
AGTATTA450.005002670530.1732115
Produced by FastQC (version 0.11.5)