FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202518
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC62850.5226532991605947No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAACCATTCAGATTGATATCCCATG57820.48082440346007294No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGCGACCC47240.39284235246374694No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCT42670.3548387633282828No Hit
GATTATTACTGTTCAGCATGGGATATCAATCTGAATGGTTGGGTTTTCGG39000.3243194696461924No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGGCCACTGATGGCCAGGGAGG37450.3114298496987155No Hit
GTGCTGACTCAGCCCCCCTCAGCGTCTGCGACCCCCGGGCAGTGGGTCAC35740.29720968833730554No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35370.2941328113175853No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34990.2909727754594942No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTC32930.27384205475510554No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28450.23658689516497883No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27520.22885312319649267No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26710.2221172572884564No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25300.21039186107817098No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24410.20299072446316813No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24360.20257493027131404No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24310.20215913607945993No Hit
ATTCTATGCCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCC23070.19184744012147845No Hit
TCATTACTATGGATGAGGAGCTGGGGGGCCGCTCCTGGGAGTTGCTGATA22850.19001794567732042No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22330.18569368608203785No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22280.18527789189018376No Hit
TAGTAATGATGCGCGGCCCTCGGGGGTCCCTGACCGATTCTATGCCTCCAGGTCTGGCACCTCAGCCTCCCTGGC21750.18087047345653037No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21630.17987256739608054No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21620.17978940855770975No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAG21540.1791241378507432No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21500.17879150249725992No Hit
GCCCTCGGGGGTCCCTGACCGATTCTATGCCTCCAGGTCTGGCACCTCAG21360.17762727876006845No Hit
GTCTGAGGATGAGGCTGATTATTACTGTTCAGCATGGGATATCAATCTGAATGGTTGGGTTTTCGGCGGAGGGAC20710.17222195426596526No Hit
TCCTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGGCATAGAATCGGTCAGGGACC20490.17039245982180723No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGACTCA20140.1674819004788286No Hit
GTAATGATGCGCGGCCCTCGGGGGTCCCTGACCGATTCTATGCCTCCAGG20020.16648399441837877No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19730.16407238810562502No Hit
GGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGACTCACTGTGCAGGGTCCTGGGCCCAGT19130.15908285780337592No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18800.1563386161371389No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT18430.1532617391174186No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17760.14769009694657376No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17720.14735746159309052No Hit
CATCATTACTATGGATGAGGAGCTGGGGGGCCGCTCCTGGGAGTTGCTGA17670.1469416674012364No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17540.14586060250241575No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17420.14486269644196592No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAC16900.14053843684668338No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16760.1393742131094919No Hit
CTCCTGGGAGTTGCTGATACCAGTTTACATTGTTACTTCCGATGTTGGAACTGCTTCCAAAACAAGAAATGGTGA16610.13812683053392963No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16340.13588154189791754No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTC15930.13247202952471399No Hit
ACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGACTCACTGTGCAGGGTCCTGGGC15820.13155728230263497No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15710.13064253508055595No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15260.12690038735386913No Hit
ATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG15170.12615195780853178No Hit
GGCCATCAGTGGCCTCCAGTCTGAGGATGAGGCTGATTATTACTGTTCAG15120.12573616361667767No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15030.1249877340713403No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTC14910.12398982801089048No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14890.12382351033414885No Hit
CCTGACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCC14800.12307508078881148No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14780.12290876311206984No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14710.12232665124347411No Hit
GACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAACCATTCAGA14670.12199401588999083No Hit
GTTCCAACATCGGAAGTAACAATGTAAACTGGTATCAGCAACTCCCAGGA14500.12058031563768692No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14470.12033083912257447No Hit
CCCTCAGCGTCTGCGACCCCCGGGCAGTGGGTCACCATTTCTTGTTTTGG14410.11983188609234956No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14330.11916661538538302No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14290.11883398003189974No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14210.11816870932493319No Hit
AGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGACTCACTGTGCAGGGT14190.11800239164819154No Hit
GCTTGGTCCCTCCGCCGAAAACCCAACCATTCAGATTGATATCCCATGCTGAACAGTAATAATCAGCCTCATCCT14070.11700448558774172No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14040.11675500907262926No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13830.11500867346684207No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTC13760.11442656159824634No Hit
GTAGTAAACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGTTTCATGGAAAACTGATTATTGGACGCGTCCACCGA13700.11392760856802142No Hit
CCCGAGGGCCGCGCATCATTACTATGGATGAGGAGCTGGGGGGCCGCTCC13690.11384444972965062No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13680.1137612908912798No Hit
AGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGCGACCCCCGGGCAGTGG13370.11118336690178442No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13150.10935387245762643No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13070.10868860175065986No Hit
TCCCTCTCCTCCTCACCCTCCTGACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAG13020.10827280755880578No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTC12880.1071085838216143No Hit
CTCCCAGGAGCGGCCCCCCAGCTCCTCATCCATAGTAATGATGCGCGGCC12880.1071085838216143No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12490.10386538912515238No Hit
GTGCCAGACCTGGAGGCATAGAATCGGTCAGGGACCCCCGAGGGCCGCGC12430.10336643609492747No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGGCATAGAATCGGTC12420.10328327725655667No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGA12370.10286748306470256No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12200.10145378281239865No Hit
GACCGATTCTATGCCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCAGTCTGAGGATGAGG12110.10070535326706129No Hit
GCATGGGATATCAATCTGAATGGTTGGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGG12060.1002895590752072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)