FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202518
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC58900.4898055580041213No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAACCATTCAGATTGATATCCCATG50640.42111635750982523No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGCGACCC46110.3834454037278444No Hit
GATTATTACTGTTCAGCATGGGATATCAATCTGAATGGTTGGGTTTTCGG40280.3349638009576572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40220.3344648479274323No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCT38630.32124259262647215No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35670.29662757646870985No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGGCCACTGATGGCCAGGGAGG34170.28415375071308707No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34050.2831558446526372No Hit
GTGCTGACTCAGCCCCCCTCAGCGTCTGCGACCCCCGGGCAGTGGGTCAC33770.2808273971782543No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTC29830.24806281486015178No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26520.22053723935941083No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26170.2176266800164322No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24880.20689918986659658No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24530.20398863052361796No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23820.1980843529992898No Hit
ATTCTATGCCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCC23460.19509063481794037No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAG23410.19467484062608628No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23310.19384325224237808No Hit
TAGTAATGATGCGCGGCCCTCGGGGGTCCCTGACCGATTCTATGCCTCCAGGTCTGGCACCTCAGCCTCCCTGGC22550.18752318052619588No Hit
GTCTGAGGATGAGGCTGATTATTACTGTTCAGCATGGGATATCAATCTGAATGGTTGGGTTTTCGGCGGAGGGAC22020.18311576209254246No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21390.1778767552751809No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21370.17771043759843927No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21220.17646305502287699No Hit
GCCCTCGGGGGTCCCTGACCGATTCTATGCCTCCAGGTCTGGCACCTCAG21140.17579778431591045No Hit
TCATTACTATGGATGAGGAGCTGGGGGGCCGCTCCTGGGAGTTGCTGATA20920.17396828987175245No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20500.17047561866017807No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20290.1687292830543909No Hit
GTAATGATGCGCGGCCCTCGGGGGTCCCTGACCGATTCTATGCCTCCAGG20230.16823033002416596No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20080.1669829474486037No Hit
TCCTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGGCATAGAATCGGTCAGGGACC19970.16606820022652466No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGACTCA19460.16182709946961293No Hit
GGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGACTCACTGTGCAGGGTCCTGGGCCCAGT18800.1563386161371389No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18390.15292910376393534No Hit
CATCATTACTATGGATGAGGAGCTGGGGGGCCGCTCCTGGGAGTTGCTGA17730.1474406204314613No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17600.14635955553264068No Hit
CTCCTGGGAGTTGCTGATACCAGTTTACATTGTTACTTCCGATGTTGGAACTGCTTCCAAAACAAGAAATGGTGA17510.1456111259873033No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17250.14344899618966203No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17040.14170266058387485No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16210.1348004769990969No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16100.1338857297770179No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15780.1312246469491517No Hit
ACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGACTCACTGTGCAGGGTCCTGGGC15630.12997726437358942No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15530.12914567598988125No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15290.12714986386898158No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTC15150.12598564013179012No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14920.1240729868492613No Hit
GGCCATCAGTGGCCTCCAGTCTGAGGATGAGGCTGATTATTACTGTTCAG14820.12324139846555311No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14760.1227424454353282No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14710.12232665124347411No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14660.12191085705162003No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTC14650.1218276982132492No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAC14600.12141190402139511No Hit
ATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14520.12074663331442856No Hit
CCTGACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCC14500.12058031563768692No Hit
GTAGTAAACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGTTTCATGGAAAACTGATTATTGGACGCGTCCACCGA14340.11924977422375382No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14340.11924977422375382No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14170.1178360739714499No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTC14140.11758659745633745No Hit
AGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGACTCACTGTGCAGGGT13580.1129297025075716No Hit
GTTCCAACATCGGAAGTAACAATGTAAACTGGTATCAGCAACTCCCAGGA13550.11268022599245915No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13440.11176547877038015No Hit
CTCCCAGGAGCGGCCCCCCAGCTCCTCATCCATAGTAATGATGCGCGGCC13370.11118336690178442No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTGA13360.11110020806341361No Hit
GCTTGGTCCCTCCGCCGAAAACCCAACCATTCAGATTGATATCCCATGCTGAACAGTAATAATCAGCCTCATCCT13280.11043493735644705No Hit
CCCTCAGCGTCTGCGACCCCCGGGCAGTGGGTCACCATTTCTTGTTTTGG13210.10985282548785133No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13080.10877176058903068No Hit
CCCGAGGGCCGCGCATCATTACTATGGATGAGGAGCTGGGGGGCCGCTCC13050.10852228407391823No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTC12740.10594436008442285No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12500.10394854796352321No Hit
GCATGGGATATCAATCTGAATGGTTGGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGG12470.10369907144841076No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12430.10336643609492747No Hit
GACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAACCATTCAGA12270.10203589468099439No Hit
AGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGCGACCCCCGGGCAGTGG12220.10162010048914027No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12170.10120430629728619No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12170.10120430629728619No Hit
TTGATATCCCATGCTGAACAGTAATAATCAGCCTCATCCTCAGACTGGAG12050.10020640023683637No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC12040.10012324139846555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)