FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756617
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29130.3850032447063706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29070.38421024111274266No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25810.3411237125256239No Hit
GTAATAGACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA25700.3396698726039727No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19510.257858335194689No Hit
AGCCTGGAGAGTCCCTGAGACTCGCCTGTGTAGCCTCTGGATTCAGCCTC19200.25376114996094457No Hit
GTCTATTACTGCGCGAGAGATCGACTGGTACCAGCGGTCCACTGGGGCCA17200.22732769684001286No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15230.2012907455158951No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14920.19719356028215068No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14550.19230337145477833No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14340.18952785887708046No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13580.17948314669112642No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13520.17869014309749848No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13330.17617896505100994No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13260.17525379419177733No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12940.17102444169242828No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12580.16626642013066056No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12240.16177273310010218No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12040.159129387788009No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCTTGTT11590.15318186083579938No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11580.1530496935701947No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11520.15225668997656674No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11390.15053851552370617No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11310.1494811773988689No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11260.14882034107084563No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11240.1485560065396363No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11130.14710216661798506No Hit
GCACTGAACACAGAGGACTCACCATGGAGTCTGGGCTGAACTGGGTTTTC11120.1469699993523804No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11090.14657349755556642No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10820.14300498138424064No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10440.13798262529126362No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA9970.13177076380784467No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTG9810.1296560875581701No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9770.1291274184957515No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9700.12820224763651886No Hit
CTCCAGGCTTGACCAAGGTTCCCCCAGACTCCACCAGCTGCACCTGACTC9640.12740924404289092No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9580.12661624044926295No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9500.1255589023244257No Hit
ACCATATATTACGCAGACTCTGTGAAGGGCCGATTCTCCATCTCCAGGGACAACGCCAAGAATTGTCTGTATCTG9480.12529456779321638No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCC9350.12357639334035582No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9040.11947920810661139No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8940.11815753545056482No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8710.11511768834165767No Hit
CTCCAGGGACAACGCCAAGAATTGTCTGTATCTGCAAATGAACAGCCTGAGAGCCGACGACACGGCCGTCTATTA8660.11445685201363438No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8470.11194567396714586No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGAT8400.11102050310791325No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8340.1102274995142853No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8280.10943449592065735No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7960.10520514342130827No Hit
GGGTTCCCTGGCCCCAGTGGACCGCTGGTACCAGTCGATCTCTCGCGCAGTAATAGACGGCCGTGTCGTCGGCT7840.10361913623405236No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC7760.1025617981092151No Hit
GAGTATTAGTACAAACTTAGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC7670.10137229271877317No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7650.10110795818756386No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7630.10084362365635453No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7590.10031495459393591No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC7580.10018278732833125No Hit
CTACTACATGAGCTGGATTCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGA7580.10018278732833125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)