FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606612
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55940.3481861208555644No Hit
GGGTCTCTCTCCCAGCTCATGCTGACTCAATCGCCCTCTGCCTCTGCCTC55050.3426465132838545No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA49960.3109649373962102No Hit
GTCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT49500.3081017694377983No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48720.3032468324648391No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44840.279096633163452No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT42870.2668348051676447No Hit
GTAATATACGGCCGTGTCCGCGGCTGTCAGGCTGTTCATTTGCAGATACA42550.26484303615309734No Hit
GTGTTTACCTTCATCAAGTAACGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC37770.2350909864982958No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG37330.23235230410329316No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36450.22687493931328784No Hit
GCTCTGGGACTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC35210.21915683438191672No Hit
GGTAAACACTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT34380.2139906835004345No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC33980.21150097223225023No Hit
CTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAG32550.2026002544484916No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG30530.19002721254416124No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30490.18977824141734284No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT30430.1894047847271152No Hit
GCACAGGGTCTCTCTCCCAGCTCATGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTTGGTCAAG30160.18772422962109084No Hit
CCCAAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCA29610.18430087662733752No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC29200.1817489225774487No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTCATGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC28580.17788987011176313No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27820.1731594187022131No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27610.1718523202864164No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26870.16724635444027555No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26650.16587701324277423No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26620.1656902848976604No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26310.16376075866481762No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25730.1601506773259505No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25600.15934152116379063No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24640.15336621412014848No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG24030.14956940443616754No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23780.1480133348935524No Hit
GGATACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCC23770.1479510921118478No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA23690.14745314985821095No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTCATGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTTGGT23610.1469552076045741No Hit
CTCCCAGCTCATGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTTGGTCAAGCTCACGTGCACT23540.14651950813264186No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC23420.1457725947521866No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23410.145710351970482No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT23010.14322064070229776No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22980.14303391235718393No Hit
GTAGTATCCCTCCCAGCCACTGCTACCCCGGGCCCCTTTCGTACAGTAAT22800.14191354228650102No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22800.14191354228650102No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22640.14091765777922735No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22610.14073092943411353No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22060.1373075764403602No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22000.13693411975013256No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21330.13276385337592397No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTCATGCTGACTCAATCGCCCTCTGC21150.1316434833052411No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21060.13108329826989965No Hit
CTGCTACCCCGGGCCCCTTTCGTACAGTAATATACGGCCGTGTCCGCGGC20880.12996292819921673No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCATTTGGGTGTTCGGCGGAGGGACCAAG20770.12927825760046607No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20720.12896704369194303No Hit
GGGTAGCAGTGGCTGGGAGGGATACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAG20630.1284068586566016No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC20540.12784667362126012No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20520.12772218805785093No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC20430.1271620030225095No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20340.12660181798716802No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGACTGAGCGCTACCTCACCATCTCCAG20310.1264150896420542No Hit
GTATATTACTGTACGAAAGGGGCCCGGGGTAGCAGTGGCTGGGAGGGATA20140.12535696235307592No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGCTGGAGC20070.12492126288114368No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG19840.12348967890193775No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19620.12212033770443642No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19620.12212033770443642No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19480.12124893876057194No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19300.12012856868988903No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19210.11956838365454758No Hit
GTCTCTCTCCCAGCTCATGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTTGGTCAAGCTCACGTG18840.11726540073147718No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18790.11695418682295415No Hit
CGTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG18410.11458896111817914No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC18350.11421550442795149No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC18000.1120370070682903No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17930.11160130759635806No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17740.11041869474397055No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17700.11016972361715212No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17620.10967178136351528No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17420.10842692572942317No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17180.10693309896851262No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTCATGCTGACTCAATCGCCCTCTGCCTCTGCCT17120.10655964227828497No Hit
GAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTA17110.10649739949658038No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17110.10649739949658038No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17050.10612394280635273No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT16600.10332301762964549No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATGCCATGAGCTGGGTCCG16410.10214040477725798No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16410.10214040477725798No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16070.10002415019930139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACCG150.002521443667.8269654
GCGGTAT200.00783994950.9288671
CGAAATC305.881433E-446.6644449
GTATTAT350.001458222938.8029441
ACGAGTC350.001465382238.7642979
GACAGGT2900.038.6356931
GTATCAA23750.038.3128051
GGTATCA9600.037.4893041
CTCGGTA400.002795618333.98434427
GGTATAA400.002808464533.952581
GCTTGTA1350.032.6950761
TGGTATC3750.032.5589682
TATCAAC27800.032.4516072
ATCAACG28300.031.7564413
TCAACGC28400.031.6446194
GCTTCGT652.5903153E-531.3408411
CAACGCA29100.031.0038095
ACTAGAA553.5427016E-430.91800128
AACGCAG29150.030.7188786
GTGGTAT4000.030.557321
Produced by FastQC (version 0.11.5)