FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994185
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40790.4102858119967611No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36750.3696495119117669No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26590.26745525229207845No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23700.23838621584513947No Hit
CTCCTAAACTACTCATTTCCTGGGCATCTATTCGGGAATCCGGGGTCCCT23560.23697802722833275No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23360.23496632920432312No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22700.22832772572509138No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21380.21505051876662792No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20870.2099206888054034No Hit
GTTTATTACTGTCACCAATATTTTAGTACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20840.209618934101802No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20780.20901542469459908No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20700.20821074548499524No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19680.19795108556254623No Hit
GTAATAAGCACCCGTGTCGTCGCCTCTCAGATTATCCATTTTAAGATATA18860.18970312366410677No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18800.1890996142569039No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT18800.1890996142569039No Hit
GTTTAGGAGGCTGGCCTGGCTTCTTGAGATACCAACCCATGTAGATCTTA18150.18256159567887265No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17670.17773352042124957No Hit
GATCTACATGGGTTGGTATCTCAAGAAGCCAGGCCAGCCTCCTAAACTAC17070.17169842634922072No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16740.16837912460960486No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGGTCTCTGGTAC16420.16516040777118948No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACTTGCAAGTCCAGCCAGAATCTTTTGTT16310.16405397385798418No Hit
CTCCTGTGGAGCCTCTGAATCCACCTTCAGCAGTTATTGGATACACTGGGTCCGTCAAGCTCCAGGCAAGGGG16150.16244461543877647No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16040.1613381815255712No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15930.16023174761236592No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15810.15902472879796015No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15770.15862238919315821No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15740.15832063448955677No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15620.157113615675151No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15190.15278846492353032No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14770.14856389907311013No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14590.1467533708515015No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14490.14574752183949669No Hit
GCTTATTACTGTGCAGGCTCGGGGGGAATTCCGCAGTGGTTGATCTGGGGCAAAGGGACCACAGTCACCGTCTC14480.1456469369382962No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG14420.1450434275310933No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACTTGCAAGTCCAGCCAGAATCTTTTGTTCACCTCCAA14410.14494284262989282No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC13840.13920950326146542No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13840.13920950326146542No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13750.1383042391506611No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13610.13689605053385437No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTTTAGTACT13590.1366948807314534No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13460.13538727701584716No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13430.13508552231224571No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13330.1340796733002409No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13160.1323697299798327No Hit
CCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13140.13216856017743175No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13040.13116271116542694No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12970.1304586168570236No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12460.12532878689579907No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12410.12482586238979666No Hit
CCTCCAACTCTAACAGTAAGATCTACATGGGTTGGTATCTCAAGAAGCCAGGCCAGCCTCCTAAACTACTCATTT12140.1221100700573837No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12010.12080246634177744No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11990.12060129653937647No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11900.11969603242857214No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11890.11959544752737165No Hit
GTCAGGGACCCCGGATTCCCGAATAGATGCCCAGGAAATGAGTAGTTTAGGAGGCTGGCCTGGCTTCTTGAGATA11570.11637673068895628No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11560.11627614578775579No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11550.11617556088655533No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11520.11587380618295387No Hit
ATGTAGATCTTACTGTTAGAGTTGGAGGTGAACAAAAGATTCTGGCTGGACTTGCAAGTGATGGTGGCCCTCTCG11490.11557205147935243No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11360.11426444776374618No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACTTGCAAGTCCAGCCAGAATCTTTTGTTCACCTCCAACT11190.112554504443338No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11180.11245391954213754No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC11060.11124690072773177No Hit
GGTTGGTATCTCAAGAAGCCAGGCCAGCCTCCTAAACTACTCATTTCCTGGGCATCTATTCGGGAATCCGGGGTC11050.11114631582653128No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11030.11094514602413032No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC11000.11064339132052887No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11000.11064339132052887No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10980.11044222151812791No Hit
GGGTCTCTGGTACCTTCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG10900.10963754230852406No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10860.10923520270372214No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC10740.10802818388931637No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10730.10792759898811591No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10680.1074246744821135No Hit
CTATTAATACGTGAGACCCACACCAGCCCCTTGCCTGGAGCTTGACGGAC10660.10722350467971253No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC10610.10672058017371013No Hit
GTCTCTGGGCGAGAGGGCCACCATCACTTGCAAGTCCAGCCAGAATCTTT10580.10641882547010868No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10460.10521180665570291No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10430.10491005195210146No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10400.10460829724850003No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT10270.10330069353289377No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10250.10309952373049282No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC10180.10239542942208944No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10030.10088665590408223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)