FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994185
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42320.42567530188043473No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36890.3710577005285737No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34710.34913019206686885No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26130.2628283468368563No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22020.22148795244345873No Hit
GTTTATTACTGTCACCAATATTTTAGTACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20980.2110271227186087No Hit
CTCCTAAACTACTCATTTCCTGGGCATCTATTCGGGAATCCGGGGTCCCT20690.20811016058379478No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20640.20760723607779236No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20530.20650080216458705No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20530.20650080216458705No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20490.20609846255978514No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA19190.19302242540372266No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18340.1844727088016818No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18220.18326568998727602No Hit
GTAATAAGCACCCGTGTCGTCGCCTCTCAGATTATCCATTTTAAGATATA17810.17914170903805632No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17560.17662708650804426No Hit
GTTTAGGAGGCTGGCCTGGCTTCTTGAGATACCAACCCATGTAGATCTTA16950.17049140753481495No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16500.16596508698079332No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16190.1628469550435784No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16110.16204227583397457No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16070.16163993622917264No Hit
CTCCTGTGGAGCCTCTGAATCCACCTTCAGCAGTTATTGGATACACTGGGTCCGTCAAGCTCCAGGCAAGGGG15850.15942706840276205No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGGTCTCTGGTAC15340.15429723844153756No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15290.15379431393553514No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15180.15268788002232983No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14940.15027384239351832No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14920.15007267259111734No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14740.14826214436950869No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14650.14735688025870436No Hit
GCTTATTACTGTGCAGGCTCGGGGGGAATTCCGCAGTGGTTGATCTGGGGCAAAGGGACCACAGTCACCGTCTC14260.14343406911188564No Hit
GATCTACATGGGTTGGTATCTCAAGAAGCCAGGCCAGCCTCCTAAACTAC14230.14313231440828417No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14100.1418247106926779No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13980.14061769187827214No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACTTGCAAGTCCAGCCAGAATCTTTTGTT13850.1393100881626659No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13820.13900833345906446No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC13490.1356890317194486No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13360.13438142800384234No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTTTAGTACT13350.13428084310264185No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG13320.13397908839904044No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGAAT13110.1318668054738303No Hit
CCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13060.13136388096782792No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACTTGCAAGTCCAGCCAGAATCTTTTGTTCACCTCCAA12870.1294527678450188No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12640.12713931511740773No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12330.12402118318019283No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA12290.1236188435753909No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12220.12291474926698753No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG12200.12271357946458657No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12190.1226129945633861No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11940.12009837203337406No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11720.1178855042069635No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11720.1178855042069635No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11220.11285625914693946No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11210.11275567424573897No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11210.11275567424573897No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11180.11245391954213754No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11150.11215216483853609No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10850.10913461780252168No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10820.10883286309892022No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10760.10822935369171732No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10760.10822935369171732No Hit
GTCAGGGACCCCGGATTCCCGAATAGATGCCCAGGAAATGAGTAGTTTAGGAGGCTGGCCTGGCTTCTTGAGATA10620.1068211650749106No Hit
GGGTCTCTGGTACCTTCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG10580.10641882547010868No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10570.10631824056890819No Hit
ATGTAGATCTTACTGTTAGAGTTGGAGGTGAACAAAAGATTCTGGCTGGACTTGCAAGTGATGGTGGCCCTCTCG10570.10631824056890819No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10470.1053123915569034No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC10430.10491005195210146No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10330.10390420294009667No Hit
CTATTAATACGTGAGACCCACACCAGCCCCTTGCCTGGAGCTTGACGGAC10290.10350186333529474No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC10280.10340127843409426No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10150.102093674718488No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10140.10199308981728754No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT10110.10169133511368608No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC10050.10108782570648321No Hit
CCTCCAACTCTAACAGTAAGATCTACATGGGTTGGTATCTCAAGAAGCCAGGCCAGCCTCCTAAACTACTCATTT10030.10088665590408223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAC200.00799718650.6722377
GGTATCA8400.040.2403531
GTGGTAT3050.038.7890621
GTATCAA20150.037.4085541
ATTAGGT552.6236346E-432.5406357
TATCAAC24350.031.0699462
ATCAACG24450.030.5269223
TCAACGC24400.030.4525954
TAACCGT703.2132542E-530.36553453
AACGCAG24750.030.3009746
CAACGCA24650.030.2822845
GACAGGT2050.029.6797161
CGTGCAA853.3230972E-629.47798557
GGTAATC500.00605123729.02251861
ACGCAGA25900.028.9555647
CGCAGAG26300.028.5151758
AGAGTAC26650.027.76039911
GCAGAGT27100.027.5487449
CAGAGTA26950.027.4513810
GAGTTTA4100.027.2064061
Produced by FastQC (version 0.11.5)