FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742767
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32950.44361152285979316No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28300.3810077722892912No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21880.29457420698550146No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20540.27653355628346443No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20000.26926344331398677No Hit
TGTTGATACTTACCCTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT19980.2689941798706728No Hit
GTGGAGTCCCATCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCATT19560.263339647561079No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18700.25176131949857766No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18490.24893405334378074No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTACAACTTTTTTAGCCTGGTATCAGCAAAAA18300.2463760506322979No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTACAA18270.2459721554673269No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17220.2318358246933426No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16960.22833539993026078No Hit
AGTCAGGACATTACAACTTTTTTAGCCTGGTATCAGCAAAAACCAGGGAAGGCCCCTAAACTCCTGATCCATT16000.21541075465118942No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15860.21352591054799147No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15850.21339127882633452No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15760.21217959333142156No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15730.2117756981664506No Hit
GTATCAACATGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTCTGAGAATGAATTCTGTC15670.21096790783650862No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15490.20854453684668273No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15060.20275537281543204No Hit
GTCTGGGACAGAATTCATTCTCAGAATCAGCGGCCTGCAGCCTGAAGATT15060.20275537281543204No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14710.19804326255743726No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14040.1890229372064187No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13700.18444545867008091No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT13410.18054113874202812No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13370.18000261185540015No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG13110.17650218709231832No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13060.17582902848403334No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13050.17569439676237636No Hit
GAAATAGACAGCCGTGTCTGCGGCGGTCACAGACCTCAACTCCAGGGAGT11930.1606156439367931No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC11890.1600771170501651No Hit
GGACTCCACTTTGCAAAGTGGATGCAGAATGGATCAGGAGTTTAGGGGCCTTCCCTGGTTTTTGCTGATACCAGG11890.1600771170501651No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11660.1569805874520543No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC11440.15401868957560044No Hit
ACTTATTACTGTCAACATGTTGATACTTACCCTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA11290.15199921375074552No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11210.1509221599774896No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11100.14944121103926267No Hit
CCCCTAAACTCCTGATCCATTCTGCATCCACTTTGCAAAGTGGAGTCCCA11020.1483641572660067No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10900.14674857660612278No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10800.14540225938955284No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10720.1443252056162969No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10690.14392131045132592No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10640.14324815184304096No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10220.13759361953344723No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10180.13705509264681925No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9790.13180445550219652No Hit
GTTTAGGGGCCTTCCCTGGTTTTTGCTGATACCAGGCTAAAAAAGTTGTA9720.13086203345059755No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9710.13072740172894057No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9640.12978497967734162No Hit
CTCCTGATCCATTCTGCATCCACTTTGCAAAGTGGAGTCCCATCCAGGTT9620.12951571623402763No Hit
GTCTATTTCTGTGCGGGATATTATGACTCATACGGACGGCGGTTCTTCGA9620.12951571623402763No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTCTGAGAATGAATTCTGTCCCAGACCCACTGCCG9600.12924645279071365No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9440.12709234524420174No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9380.12628455491425977No Hit
GTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGAAGGATGGAGACTGGGTCAA9350.12588065974928883No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9270.12480360597603285No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9180.12359192048111993No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9140.12305339359449194No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8920.1200914957180381No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8890.11968760055306711No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8830.11887981022312515No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8760.1179373881715262No Hit
GTGGATGCAGAATGGATCAGGAGTTTAGGGGCCTTCCCTGGTTTTTGCTG8710.11726422956324122No Hit
GTCCCATCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCATTCTCAG8660.11659107095495626No Hit
ATTCTGCATCCACTTTGCAAAGTGGAGTCCCATCCAGGTTCAGCGGCAGT8660.11659107095495626No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8560.11524475373838633No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8520.11470622685175835No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8450.11376380480015941No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8420.11335990963518842No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8410.11322527791353143No Hit
GAATTCATTCTCAGAATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACATGTTGATACTTAC8390.11295601447021744No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8380.11282138274856045No Hit
CCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG8370.11268675102690347No Hit
GGATGGGACTCCACTTTGCAAAGTGGATGCAGAATGGATCAGGAGTTTAGGGGCCTTCCCTGGTTTTTGCTGATA8350.11241748758358946No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8280.11147506553199052No Hit
CTGCATCCACTTTGCAAAGTGGAGTCCCATCCAGGTTCAGCGGCAGTGGG8280.11147506553199052No Hit
GATCAGGAGTTTAGGGGCCTTCCCTGGTTTTTGCTGATACCAGGCTAAAA8230.11080190692370556No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8190.11026338003707757No Hit
GGACATTACAACTTTTTTAGCCTGGTATCAGCAAAAACCAGGGAAGGCCCCTAAACTCCTGATCCATTCTGCATC8180.11012874831542059No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8090.10891706282050764No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8080.10878243109885065No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8040.10824390421222269No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8000.10770537732559471No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA7940.10689758699565274No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7930.10676295527399574No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7880.10608979666571078No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7860.10582053322239679No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7780.10474347944914085No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC7770.10460884772748386No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7770.10460884772748386No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7750.10433958428416987No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG7710.10380105739754189No Hit
GGACTGGAGTGGCTTGGGAATATCTATTATACTGGAAATACCTACTACAA7680.10339716223257091No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7590.10218547673765799No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7590.10218547673765799No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7570.10191621329434397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)