FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742767
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32030.4312254044673498No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24060.32392392230672606No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20650.27801450522169135No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19600.263878174447707No Hit
TGTTGATACTTACCCTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT19030.25620416631325843No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18880.2541846904884035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17660.2377596204462503No Hit
GTGGAGTCCCATCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCATT17510.23574014462139542No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17300.23291287846659856No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17230.23197045641499958No Hit
GTATCAACATGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTCTGAGAATGAATTCTGTC16530.22254623589901004No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15890.21392980571296247No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTACAACTTTTTTAGCCTGGTATCAGCAAAAA15890.21392980571296247No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15880.2137951739913055No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTACAA15750.21204496160976455No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14900.20060126526892014No Hit
AGTCAGGACATTACAACTTTTTTAGCCTGGTATCAGCAAAAACCAGGGAAGGCCCCTAAACTCCTGATCCATT14620.19683157706252433No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14550.19588915501092535No Hit
GTCTGGGACAGAATTCATTCTCAGAATCAGCGGCCTGCAGCCTGAAGATT14530.19561989156761136No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14510.1953506281242974No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13960.18794588343316276No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13910.18727272482487778No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13770.1853878807216799No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG13630.18350303661848197No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13420.1806757704636851No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13180.17744460914391727No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12700.17098228650438158No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12590.16950133756615465No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12420.16721259829798577No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT12400.16694333485467178No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGATGGGACTCCACTTTGCAAAGT12250.16492385902981688No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12210.16438533214318893No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11780.1585961681119382No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11700.15751911433868224No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11690.15738448261702526No Hit
GAAATAGACAGCCGTGTCTGCGGCGGTCACAGACCTCAACTCCAGGGAGT11470.15442258474057138No Hit
GGACTCCACTTTGCAAAGTGGATGCAGAATGGATCAGGAGTTTAGGGGCCTTCCCTGGTTTTTGCTGATACCAGG11450.15415332129725742No Hit
ACTTATTACTGTCAACATGTTGATACTTACCCTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA11260.15159531858577455No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10950.14742173521440774No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10780.14513299594623885No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC10330.13907456847167415No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC10330.13907456847167415No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10090.13584340715190632No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9960.1340931947703654No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTCTGAGAATGAATTCTGTCCCAGACCCACTGCCG9940.13382393132705142No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9930.13368929960539444No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9880.13301614099710946No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9820.13220835066716752No Hit
CCCCTAAACTCCTGATCCATTCTGCATCCACTTTGCAAAGTGGAGTCCCA9670.1301888748423126No Hit
CTCCTGATCCATTCTGCATCCACTTTGCAAAGTGGAGTCCCATCCAGGTT9570.12884255762574268No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9290.12507286941934687No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9280.12493823769768986No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9160.12332265703780593No Hit
GAATTCATTCTCAGAATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACATGTTGATACTTAC9110.12264949842952097No Hit
GTCTATTTCTGTGCGGGATATTATGACTCATACGGACGGCGGTTCTTCGA9040.12170707637792202No Hit
GTTTAGGGGCCTTCCCTGGTTTTTGCTGATACCAGGCTAAAAAAGTTGTA8980.12089928604798006No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8890.11968760055306711No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8860.11928370538809613No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8840.11901444194478215No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8830.11887981022312515No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8820.11874517850146817No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8540.11497549029507236No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8490.11430233168678737No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8460.11389843652181639No Hit
GTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGAAGGATGGAGACTGGGTCAA8400.11309064619187445No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8310.1118789606969615No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8310.1118789606969615No Hit
GTGGATGCAGAATGGATCAGGAGTTTAGGGGCCTTCCCTGGTTTTTGCTG8150.10972485315044961No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8100.10905169454216462No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8080.10878243109885065No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7980.10743611388228072No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7870.1059551649440538No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7840.10555126977908282No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7830.10541663805742581No Hit
GTCCCATCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCATTCTCAG7820.10528200633576881No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7810.10514737461411183No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC7800.10501274289245482No Hit
CTGCATCCACTTTGCAAAGTGGAGTCCCATCCAGGTTCAGCGGCAGTGGG7700.10366642567588491No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7610.10245474018097196No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7590.10218547673765799No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7570.10191621329434397No Hit
GGATGGGACTCCACTTTGCAAAGTGGATGCAGAATGGATCAGGAGTTTAGGGGCCTTCCCTGGTTTTTGCTGATA7560.101781581572687No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7500.10097379124274504No Hit
ATTCTGCATCCACTTTGCAAAGTGGAGTCCCATCCAGGTTCAGCGGCAGT7440.10016600091280307No Hit
GGACTGGAGTGGCTTGGGAATATCTATTATACTGGAAATACCTACTACAA7430.10003136919114607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)