FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064461
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63480.596358156851214No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56540.5311608410265853No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT43300.4067786419605791No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41030.38545329514186055No Hit
CTACAAACTCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG39960.3754012594167377No Hit
GGGGAAATAGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACGCCTGG39380.3699524923881664No Hit
GTGTAAAATCTGTGCCTGACCCACTGCCACTGAACCTGTCAGGGACCCCG39280.3690130497970334No Hit
GGTCTAGTCAGAGCCTCCTGTCTGGAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC37140.34890897834678775No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36230.34036005076747766No Hit
GATTTTACACTGAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT35560.3340657854068867No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35170.3304019593014681No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35040.3291806839329952No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTAAAAT33780.31734370728471967No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33270.31255255006994154No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTAAAATCTGTGCCTGACCCACTGCCA32000.3006216291625527No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT31230.2933879212108288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31020.2914150917694495No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30740.28878465251427715No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG30720.28859676399605055No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29640.27845078401181445No Hit
GTCTCCACTCTCCCTGCCCGTCACGCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTC29640.27845078401181445No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28900.2714989088374304No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28740.26999580069161766No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26740.2512069488689581No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26360.24763706702265278No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTA24770.23269992982363846No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTGCAGTGGGGAAATAGTGATGA24520.230351323345806No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24220.22753299557240705No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC23550.22123873021181614No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23530.22105084169358954No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGAGTTCTATCCGGGCC23140.21738701558817092No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGGTCAGGCACAGATTTTACACTGAG23140.21738701558817092No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG23110.217105182810831No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22900.21513235336945175No Hit
CGCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG22590.21222008133693956No Hit
GCTAATGCTCTGGGTCTCTGGTTGCAGTGGGGAAATAGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACGC22520.21156247152314647No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTGCAGTGGGGAAATAGTGATGACTCAGTCTCCACTCTCC22450.21090486170935338No Hit
GTCAGGGACCCCGGAGGCCCGGATAGAACTCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA22080.20742892412216135No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20940.19671927858324542No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20300.19070684599999435No Hit
CTCCCTGCCCGTCACGCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA20260.19033106896354118No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20250.19023712470442788No Hit
GTTCAGTGGCAGTGGGTCAGGCACAGATTTTACACTGAGAATCAGCAGGG19980.18770062970836884No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA19740.1854459674896497No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19480.18300341675270396No Hit
CTCCACAACTCCTGATCTATTTGAGTTCTATCCGGGCCTCCGGGGTCCCT18990.17840014805615237No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18850.17708492842856619No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18700.17567576454186673No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18520.17398476787782738No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGTACGTTCGGCCAAGGGA17860.1677844467763497No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTGCAGTGGGGAAATAGTGATGACTCAGTCTCCACT17690.16618739437142366No Hit
CCACTCTCCCTGCCCGTCACGCCTGGAGAGCCGGCCTCCATCTCCTGCAG17670.16599950585319706No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16780.15763846679211357No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16650.1564171914236407No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTTCCAGAC16620.1561353586463008No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTAAAATCTGTGCCTGACCCACTGCCA16390.15397464068669495No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16250.1526594210591088No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15980.15012292606304975No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15950.14984109328570985No Hit
GGGTCTCTGGTTGCAGTGGGGAAATAGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACGCCTGGAGAGCCGG15800.1484319293990104No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15770.1481500966216705No Hit
CTCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15770.1481500966216705No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGCGTGACGGGCAGG15710.1475864310669907No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGAGTTCTATC15620.14674093273497102No Hit
AGTCAGAGCCTCCTGTCTGGAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA15380.1444862705162519No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTGCAGTGGGGAAATAGTGATGACTCAGTCTC15190.14270132959309922No Hit
TACGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCA14970.14063455589260668No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTTCCAGACAGGAGGCTCTGA14790.13894355922856733No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14650.13762833960098117No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14580.13697072978718808No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14570.13687678552807478No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14500.1362191757142817No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14330.13462212330935563No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14310.13443423479112904No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14290.13424634627290244No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14220.13358873645910935No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14150.13293112664531626No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14010.1316159070177301No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT14010.1316159070177301No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCTCGTACGTTCGGCCAAGGGA13950.1310522414630503No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT13890.1304885759083705No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13850.13011279887191735No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGAGTTCTATCCGGGCCTCCGGGGTCCCTGACAGGTT13770.12936124479901095No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGAGTTCTATCCGGGCCTCCGGGGTCCCTGA13750.12917335628078436No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13660.12832785794876467No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13640.1281399694305381No Hit
GCCTCCACCCTGCTGATTCTCAGTGTAAAATCTGTGCCTGACCCACTGCC13520.1270126383211785No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13510.1269186940620652No Hit
ATTTGAGTTCTATCCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT13490.12673080554383861No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13400.12588530721181893No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13380.12569741869359236No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13330.12522769739802586No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13220.12419431054777959No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13190.12391247777043968No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12860.12081231721970086No Hit
CTCCTGATCTATTTGAGTTCTATCCGGGCCTCCGGGGTCCCTGACAGGTT12800.12024865166502106No Hit
GAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCTCGTACGTT12620.11855765500098173No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12570.11808793370541523No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12560.11799398944630193No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12410.11658482555960248No Hit
ATCCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGGTCAGGCAC12330.11583327148669607No Hit
CAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGAGTTCTATCCGGGC12290.11545749445024289No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12250.1150817174137897No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11960.11235733389950407No Hit
CCACAACTCCTGATCTATTTGAGTTCTATCCGGGCCTCCGGGGTCCCTGA11870.1115118355674844No Hit
GTCTGGAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC11840.11123000279014449No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11530.10831773075763228No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC11470.10775406520295248No Hit
GGCACAGATTTTACACTGAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA11030.10362051780196738No Hit
GATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAAT10850.10192952113792803No Hit
GCTGATTCTCAGTGTAAAATCTGTGCCTGACCCACTGCCACTGAACCTGT10770.10117796706502163No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10690.10042641299211526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)