FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064461
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73710.6924631339241175No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60220.5657323283802789No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50310.47263356759900077No Hit
GGGGAAATAGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACGCCTGG38510.36177934184530947No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38310.35990045666304354No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37800.35510929944826536No Hit
GTGTAAAATCTGTGCCTGACCCACTGCCACTGAACCTGTCAGGGACCCCG36990.34749981446008826No Hit
CTACAAACTCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG36480.3427086572453101No Hit
GGTCTAGTCAGAGCCTCCTGTCTGGAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC35700.3353810050344728No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32480.305130953599991No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32300.3034399569359516No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTAAAAT32170.3022186815674787No Hit
GATTTTACACTGAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT32070.3012792389763458No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31770.2984609112029469No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG30970.290945370473883No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTAAAATCTGTGCCTGACCCACTGCCA30870.29000592788275004No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT29680.27882656104826764No Hit
GTCTCCACTCTCCCTGCCCGTCACGCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTC27900.2621044829261006No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27530.2586285453389086No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27120.2547768307152634No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26120.2453824048039336No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25760.24200041147585494No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25460.23918208370245597No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25110.23589403463349057No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTA24720.23223020852807197No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24710.23213626426895864No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24510.23025737908669272No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23960.2250904448354613No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC23170.21766884836551081No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23160.2175749041063975No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22650.21278374689161933No Hit
CTCCCTGCCCGTCACGCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA22500.21137458300491985No Hit
GCTAATGCTCTGGGTCTCTGGTTGCAGTGGGGAAATAGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACGC22390.2103411961546736No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTGCAGTGGGGAAATAGTGATGA22130.20789864541772785No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGAGTTCTATCCGGGCC22050.20714709134482148No Hit
CGCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG21810.20489242912610234No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTGCAGTGGGGAAATAGTGATGACTCAGTCTCCACTCTCC21630.203201432462063No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG21330.20038310468866402No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGGTCAGGCACAGATTTTACACTGAG21250.19963155061575766No Hit
CTCCACAACTCCTGATCTATTTGAGTTCTATCCGGGCCTCCGGGGTCCCT19990.18779457396748214No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA19540.18356708230738372No Hit
GTCAGGGACCCCGGAGGCCCGGATAGAACTCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA19540.18356708230738372No Hit
GTTCAGTGGCAGTGGGTCAGGCACAGATTTTACACTGAGAATCAGCAGGG19140.17980931194285182No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18760.17623943009654652No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18250.17144827288176834No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18200.17097855158620184No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17730.16656317140787685No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17500.164402453448271No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17330.16280540104334493No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTGCAGTGGGGAAATAGTGATGACTCAGTCTCCACT17310.16261751252511836No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17300.16252356826600506No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16890.15867185364235983No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16730.15716874549654708No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCTCGTACGTTCGGCCAAGGGA16720.15707480123743378No Hit
GGGTCTCTGGTTGCAGTGGGGAAATAGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACGCCTGGAGAGCCGG16470.15472619475960134No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGTACGTTCGGCCAAGGGA16150.1517199784679758No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTAAAATCTGTGCCTGACCCACTGCCA15930.14965320476748326No Hit
CTCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15890.14927742773103006No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15820.148619817917237No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15790.1483379851398971No Hit
CCACTCTCCCTGCCCGTCACGCCTGGAGAGCCGGCCTCCATCTCCTGCAG15550.14608332292117795No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15000.14091638866994657No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14740.13847383793300083No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGAGTTCTATC14730.13837989367388753No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14510.13631311997339499No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTTCCAGAC14500.1362191757142817No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14420.1354676216413753No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14320.13452817905024234No Hit
AGTCAGAGCCTCCTGTCTGGAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA14260.13396451349556254No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14190.13330690368176945No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTTCCAGACAGGAGGCTCTGA14190.13330690368176945No Hit
TACGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCA14170.13311901516354288No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14020.1317098512768434No Hit
CTCCTGATCTATTTGAGTTCTATCCGGGCCTCCGGGGTCCCTGACAGGTT14010.1316159070177301No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT13940.130958297203937No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTGCAGTGGGGAAATAGTGATGACTCAGTCTC13940.130958297203937No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGCGTGACGGGCAGG13920.1307704086857104No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13490.12673080554383861No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13440.12626108424827212No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13200.12400642202955298No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13130.1233488122157599No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13110.1231609236975333No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGAGTTCTATCCGGGCCTCCGGGGTCCCTGACAGGTT12900.12118809425615405No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12890.12109414999704077No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGAGTTCTATCCGGGCCTCCGGGGTCCCTGA12560.11799398944630193No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12550.11790004518718863No Hit
GCCTCCACCCTGCTGATTCTCAGTGTAAAATCTGTGCCTGACCCACTGCC12430.11677271407782906No Hit
ATTTGAGTTCTATCCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT12350.11602116000492269No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12340.11592721574580939No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12120.11386044204531683No Hit
GAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCTCGTACGTT12100.11367255352709024No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12050.11320283223152375No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12030.11301494371329715No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12020.11292099945418385No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11960.11235733389950407No Hit
CAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGAGTTCTATCCGGGC11900.11179366834482428No Hit
ATCCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGGTCAGGCAC11870.1115118355674844No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11850.11132394704925778No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11740.11029056019901153No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11740.11029056019901153No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11600.10897534057142536No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11540.10841167501674558No Hit
GTCTGGAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC11430.10737828816649929No Hit
CCACAACTCCTGATCTATTTGAGTTCTATCCGGGCCTCCGGGGTCCCTGA11410.1071903996482727No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11400.1070964553891594No Hit
GCTGATTCTCAGTGTAAAATCTGTGCCTGACCCACTGCCACTGAACCTGT10940.1027750194699477No Hit
GGCACAGATTTTACACTGAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA10910.1024931866926078No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10800.10145979984236153No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10770.10117796706502163No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC10760.10108402280590834No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTACGAGG10660.10014458021477537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)