FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824192
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTAACCGCGGGTCCGGGGTCCCT36710.44540592483304864No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32560.3950535797484081No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29480.3576836465289641No Hit
GTTTTACACAGCGGCGATAATAAAAACTACTTAGGTTGGCACCAGCAGAA28120.3411826370554434No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTGCCAACCTAAGTAGTTTTTATTATCGCCGCTGTGTAAAACACT25690.3116992157167262No Hit
ATTCTGAAGCTCCTCGAAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA23440.28439975151421026No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21660.2628028420562199No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21200.25722161826370554No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20500.24872845162292279No Hit
CCATCAACAGTGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAC19960.24217658021431898No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17610.21366380649169128No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17460.21184384221152353No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17020.2065052803230315No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16820.20407866128280788No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16510.20031740177046126No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15810.19182423512967853No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15640.18976160894548844No Hit
CTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA14970.18163243516073926No Hit
GTTTATTACTGTCAGCACTATTCTGAAGCTCCTCGAAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14440.1752018947041466No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14330.17386725423202362No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14160.17180462804783353No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC13990.1697420018636434No Hit
GTGTAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGTCAGGGAGTCTGGAGA13460.1633114614070508No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13340.1618554899829166No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13330.16173415903090543No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13290.1612488352228607No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13280.16112750427084951No Hit
CACCATCAACAGTGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC13110.15906487808665942No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12990.15760890666252522No Hit
GTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACA12810.15542494952632396No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCG12770.15493962571827924No Hit
CTTCAGAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCACACTGTTGATGGTGAGAGTGAAAT12660.15360498524615623No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12570.1525130066780556No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTT12310.14935840192576488No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12000.14559714241341823No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11960.1451118186053735No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11340.13758929958068022No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11340.13758929958068022No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCACTAACGCCTGG11310.13722530672464667No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11230.13625465910855722No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11190.1357693353005125No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTAACCGCGGG11140.13516268054045658No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10810.1311587591240876No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10750.1304307734120205No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCACTATTCTGAAGCTCCTCGAAGTTTTGGCCAGGGGACCAAG10660.12933879484391986No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10660.12933879484391986No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10390.12606285913961796No Hit
CCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC10240.12424289485945023No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10160.12327224724336078No Hit
GTAATAAACTGCCACATCTTCAGCCTGCACACTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10140.12302958533933842No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9580.11623505202671222No Hit
GTCAGGGACCCCGGACCCGCGGTTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTG9500.11526440441062277No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9430.11441508774654449No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9310.11295911632241032No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGTG8970.10883386395403012No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8600.1043446187296164No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8600.1043446187296164No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATCTTC8570.10398062587358284No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8550.10373796396956048No Hit
GTGTTTTACACAGCGGCGATAATAAAAACTACTTAGGTTGGCACCAGCAGAAACCAGGACAGCCTCCTAAACTG8420.10216066159341514No Hit
GTCCTGGTTTCTGCTGGTGCCAACCTAAGTAGTTTTTATTATCGCCGCTG8260.10021936636123621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)