FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824192
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTAACCGCGGGTCCGGGGTCCCT31520.3824351607392452No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31210.3786739012268986No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26470.3211630299735984No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTGCCAACCTAAGTAGTTTTTATTATCGCCGCTGTGTAAAACACT24870.3017500776518093No Hit
ATTCTGAAGCTCCTCGAAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA24120.29265025625097063No Hit
GTTTTACACAGCGGCGATAATAAAAACTACTTAGGTTGGCACCAGCAGAA23180.2812451467619196No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21510.2609828777760522No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21470.2604975539680075No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20740.2516403944711912No Hit
CCATCAACAGTGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAC19290.2340474064295698No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18800.22810218978102187No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCG18550.22506891598074236No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16930.2054133017549309No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15750.19109624941761144No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15360.18636434228917534No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14860.18029779468861626No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14440.1752018947041466No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14300.17350326137599006No Hit
GTTTATTACTGTCAGCACTATTCTGAAGCTCCTCGAAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14180.1720472899518559No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13770.16707272091939743No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13720.1664660661593415No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13710.1663447352073303No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13620.1652527566392297No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13540.16428210902314025No Hit
CTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA13520.1640394471191179No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13460.1633114614070508No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13210.16027818760677126No Hit
CTTCAGAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCACACTGTTGATGGTGAGAGTGAAAT12860.15603160428637985No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12800.15530361857431277No Hit
CACCATCAACAGTGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC12720.15433297095822332No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC12470.15129969715794378No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTT12460.1511783662059326No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC11910.14450516384531759No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT11800.1431705233731946No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11790.1430491924211834No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11700.14195721385308277No Hit
GTAATAAACTGCCACATCTTCAGCCTGCACACTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA11400.1383172852927473No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11370.13795329243671378No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11240.13637599006056841No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCACTATTCTGAAGCTCCTCGAAGTTTTGGCCAGGGGACCAAG11140.13516268054045658No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11020.1337067091163224No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10900.13225073769218823No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10730.13018811150799814No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCACTAACGCCTGG10440.12666951389967385No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTAACCGCGGG10390.12606285913961796No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10240.12424289485945023No Hit
GTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACA10240.12424289485945023No Hit
GTGTAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGTCAGGGAGTCTGGAGA10230.12412156390743903No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10040.1218162758192266No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10000.12133095201118185No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9700.11769102345084641No Hit
TCTTCAGCCTGCACACTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC9190.11150314489827615No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8930.1083485401459854No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCACTAACGCCTGGATGAGCTGGGTCCGCCAGACTCCAGGGAAGGGG8900.10798454728995187No Hit
GTCAGGGACCCCGGACCCGCGGTTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTG8830.10713523062587359No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8770.1064072449138065No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8740.10604325205777294No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATCTTC8690.10543659729771704No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8470.10276731635347103No Hit
GCACTATTCTGAAGCTCCTCGAAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT8360.10143267588134805No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGACCCGCGGTT8270.1003406973132474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG350.005518113355.6511570
GCTAACG200.00781627850.9656223
TGGTATC3150.043.051262
GTGGTAT3200.042.414611
GGTATCA8250.040.3067361
TCGGTTT650.001261909439.9546770
GCTTGAC350.001462225638.779071
GTATCAA20700.037.5379561
TGGACTA700.001816239237.10076570
TCGTGTA400.00229714535.3706654
ACGAACC805.8775186E-835.24350752
CGTACTG400.002827072333.9049266
TATCAAC23550.032.823162
AATCGTT851.2104829E-732.4619939
ATCAACG24300.031.9496173
CGTGGTT450.00380897231.90300660
CAACGCA24500.031.5504255
TCAACGC24400.031.5407834
TTTCGTC450.004091206531.44058454
AACGCAG24700.031.4341226
Produced by FastQC (version 0.11.5)