FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320533
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64840.4910138557688449No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT54620.4136208636967043No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAAAGGCAGTACTGCTGTCCCACAC54250.4108189647664996No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG51780.3921143962324304No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG43110.3264590888679041No Hit
GGATTACCTGTGGGGGAAACGACATTGGAAGCAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC42490.3217640149848584No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41070.3110107812527214No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39460.2988187345564253No Hit
TGTATAGGGATACCAGCCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACC36280.2747375491562877No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT35800.2711026532468329No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTC34590.2619396864750824No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33770.2557300726297639No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32250.24421956891649055No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGTACTGCCTTTGTGGTATTCGG31690.23997885702212668No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACGACATTGGAAGCAAAAATGTG31550.2389186790485357No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31160.23596532612210372No Hit
CCCCTGTGGTGGTCATGTATAGGGATACCAGCCGGCCCTCTGGGATCCCT29520.2235460984314667No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29490.22331891743712576No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29170.22089565349748927No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCCCCGCTGATGGTCAGGGTGG28110.21286859169744338No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27940.2115812327295115No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27830.21074823575026147No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTGCTT26940.2040085329181474No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26300.1991620050388745No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26010.19696592209357885No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25770.1951484741388515No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAATTTCAGGGAGA25450.192725210199215No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25380.19219512121241952No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25370.1921193942143059No Hit
CTATTACTGTCAGGTGTGGGACAGCAGTACTGCCTTTGTGGTATTCGGCG24840.18810586331428295No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23500.17795844556705512No Hit
GGGTGGCCGTGTTCCCCGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCA23430.17742835658025963No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACGACATTGGAAGC23330.17667108659912323No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22790.17258182870098665No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22730.1721274667123048No Hit
GTATAGGGATACCAGCCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACC22170.16788675481794094No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT21960.1662964878575545No Hit
GACCCAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAAAGGCAGTA21850.16546349087830445No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT21660.16402467791414527No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCCCCGCTGATGGTCAGGGTGGCCGTGTTCCC21250.16091987099148602No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21130.16001114701412233No Hit
GGCCAGGCCCCTGTGGTGGTCATGTATAGGGATACCAGCCGGCCCTCTGG20990.15895096904053135No Hit
CCCTATACATGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG20920.1584208800537359No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20790.15743642907825856No Hit
CGGCCACCCTGACCATCAGCGGGGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGTA20780.1573607020801449No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20650.1563762511046676No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG20520.15539180012919027No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG20130.1524384472027583No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGC20110.152286993206531No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT20040.1517569042197355No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19560.14812200831028077No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC19300.1461531063593261No Hit
GTACTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTG19240.14569874437064428No Hit
CCTTGGTCCCTCCGCCGAATACCACAAAGGCAGTACTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCC19100.14463856639705333No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18700.14160948647250768No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18640.14115512448382583No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACC18530.1403221275045758No Hit
CAGTAATAGTCAGCCTCATCCCCGGCTTGGGCCCCGCTGATGGTCAGGGT18210.13789886356493933No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18020.13646005060078012No Hit
ACCCAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAAAGGCAGTAC17780.13464260264605277No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG17390.1316892497196208No Hit
GTCTCTGGTGGCTCCATCAATAGTTACTACTGGAACTGCATCCGGCAGCC17010.12881162379130245No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTC16870.1277514458177115No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16390.12411654990825674No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16380.12404082291014311No Hit
CCACAAAGGCAGTACTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCA16330.1236621879195749No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCATGTATAGGGAT16240.12298064493655213No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT16230.1229049179384385No Hit
GTACTGCCTTTGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA16100.12192046696296117No Hit
GTGTGGGACAGCAGTACTGCCTTTGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGG15960.1208602889893702No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15900.12040592700068836No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15850.12002729201012016No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15840.11995156501200652No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15650.11851275204784735No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA15480.11722539307991546No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15390.11654385009689269No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15360.11631666910255178No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14850.11245459219875611No Hit
GACCATCAGCGGGGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGG14820.11222741120441519No Hit
GTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACGA14490.10972842026666504No Hit
ATACATGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTGCTTCCAATGTCGTTTCC14170.10730515632702857No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTGCTTCCAATGTCGTTTCCCCCACAGGTAATCCTGGC13970.10579061636475574No Hit
CAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACGACATTGGAAGCAAAAATGTGCA13860.1049576193855057No Hit
GGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG13760.1042003494043693No Hit
CTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACC13760.1042003494043693No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13750.10412462240625565No Hit
GCCTTTGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT13690.10367026041757381No Hit
AGGCAGTACTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCG13530.10245862844775558No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13270.10048972649680092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4150.062.46588570
GGTATCA11850.045.871931
ATCTCGT3300.043.785137
CGTATGC3500.042.45535341
ACGCGTA3550.042.40062327
TCGTATG3600.042.1923340
TATACAC3500.041.9875563
TCTCGTA3450.041.9309438
GTATCAA32550.041.3821871
GCGTAGT3650.041.26085729
CGTAGTA3700.040.71717530
GACGCGT3700.040.67551826
CGCGTAG3750.040.14230728
AGACGCG3800.039.59460425
GTAATCT3650.039.493234
AATCTCG3750.038.51033836
CTTATAC3850.038.1835171
TTATACA3950.038.070822
TACACAT4050.037.9775545
TAATCTC3800.037.97035235
Produced by FastQC (version 0.11.5)