FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320533
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC62330.47200637924232114No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT52770.3996113690456808No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAAAGGCAGTACTGCTGTCCCACAC52180.395143476156976No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG46690.3535693541925874No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41500.314267042171608No Hit
GGATTACCTGTGGGGGAAACGACATTGGAAGCAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC40690.3081331553244031No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG39830.30162063348663No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38800.29382075268092506No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT36860.2791297150468788No Hit
TGTATAGGGATACCAGCCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACC36230.27435891416571945No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35490.26875511630531No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31570.23907013304476302No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31500.2385400440579675No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACGACATTGGAAGCAAAAATGTG31130.2357381451277628No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30850.23361778918058085No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30720.23263333820510357No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTC30560.2314217062352853No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGTACTGCCTTTGTGGTATTCGG30310.22952853128244427No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28890.21877529755030736No Hit
CCCCTGTGGTGGTCATGTATAGGGATACCAGCCGGCCCTCTGGGATCCCT28490.2157462176257617No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27560.20870360680119315No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26350.19954064002944263No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTGCTT25720.1947698391482833No Hit
GGGTGGCCGTGTTCCCCGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCA25700.194618385152056No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25620.1940125691671469No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25350.19196794021807861No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25080.1899233112690103No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24870.18833304430862385No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCCCCGCTGATGGTCAGGGTGG24610.1863641423576692No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAATTTCAGGGAGA23970.18151761447839623No Hit
CTATTACTGTCAGGTGTGGGACAGCAGTACTGCCTTTGTGGTATTCGGCG22610.17121874273494112No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT21980.16644794185378176No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21850.16546349087830445No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACGACATTGGAAGC21820.16523630988396354No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21610.16364604292357707No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21600.16357031592546342No Hit
GTATAGGGATACCAGCCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACC21510.16288877294244067No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG21400.16205577596319062No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT21170.1603140550065769No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21090.15970823902166775No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20630.1562247971084403No Hit
CGGCCACCCTGACCATCAGCGGGGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGTA20350.15410444116125838No Hit
GGCCAGGCCCCTGTGGTGGTCATGTATAGGGATACCAGCCGGCCCTCTGG20220.15311999018578104No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19940.1509996342385991No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG19850.15031809125557632No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCCCCGCTGATGGTCAGGGTGGCCGTGTTCCC19760.1496365482725536No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC19700.14918218628387173No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19590.1483491893046217No Hit
CCCTATACATGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG19400.14691037634046253No Hit
CCTTGGTCCCTCCGCCGAATACCACAAAGGCAGTACTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCC19250.14577447136875793No Hit
GACCCAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAAAGGCAGTA18830.14259393744798501No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGC18800.1423667564536441No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18520.14024640050646217No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT17920.13570278061964372No Hit
GTACTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTG17890.1354755996253028No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACC17650.13365815167057543No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17330.13123488773093894No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17220.1304018907516889No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16940.12828153480450696No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16890.12790289981393876No Hit
CAGTAATAGTCAGCCTCATCCCCGGCTTGGGCCCCGCTGATGGTCAGGGT16800.127221356830916No Hit
GTGTGGGACAGCAGTACTGCCTTTGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGG16730.12669126784412052No Hit
ACCCAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAAAGGCAGTAC16680.1263126328535523No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTC16550.12532818187807498No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC16260.1231320989327794No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCATGTATAGGGAT16050.12154183197239296No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16040.12146610497427933No Hit
GTCTCTGGTGGCTCCATCAATAGTTACTACTGGAACTGCATCCGGCAGCC15960.1208602889893702No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG15710.11896711403652918No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15610.11820984405539278No Hit
CCACAAAGGCAGTACTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCA15330.11608948810821085No Hit
GTACTGCCTTTGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA15120.1144992211478244No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA15030.11381767816480164No Hit
GACCATCAGCGGGGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGG14970.11336331617611979No Hit
CTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACC14950.11321186217989251No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14700.11131868722705152No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14700.11131868722705152No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14660.11101577923459693No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14590.11048569024780146No Hit
GTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACGA14220.10768379131759676No Hit
ATACATGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTGCTTCCAATGTCGTTTCC14120.10692652133646036No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14000.10601779735909668No Hit
GCCTTTGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT13900.10526052737796025No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTGCTTCCAATGTCGTTTCCCCCACAGGTAATCCTGGC13860.1049576193855057No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13690.10367026041757381No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13390.1013984504741646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.047.3275221
GTATCAA31500.039.6595081
TACTGAA10000.039.10482470
TATCAAC36300.034.1999932
TACACGC400.002748922434.1008725
ATCAACG37900.032.9311833
CAACGCA38750.032.2968255
TCAACGC38700.032.2504354
AACGCAG39150.032.0563776
ACGCAGA41300.030.2224317
CGCAGAG41600.030.004488
TCTCGCG5150.029.60049270
AGAGTAC43050.029.23153111
CAGAGTA43150.029.16378810
GCAGAGT43600.028.7845679
TCTACTG9400.026.55118668
TGTCTAC8850.026.15389466
GTCTACT9350.025.1358367
AGTACGG36250.024.27592713
GAGTACG36350.024.20639412
Produced by FastQC (version 0.11.5)