FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953816
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47010.49286235500348075No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC39890.4182148338882971No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29100.30509028995110166No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26320.27594420726848784No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26130.2739522088117624No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24660.2585404312781501No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC23920.25078212149932483No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC23570.24711265065798854No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCACCATCTCCTG22270.23348318753302522No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21680.22729750811477265No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21370.22404740536958911No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGGTGCTGGTTGTATATGAACTGCAGTAATAATCAG20950.21964404035998558No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20890.21901498821575652No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20430.21419225511000026No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19360.2029741585379151No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18890.19804658340812062No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18690.19594974292735703No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT16610.17414260192741576No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16410.17204576144665218No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16380.17173123537453763No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAC16240.17026344703800314No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16180.16963439489377405No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC15650.16407776761975057No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15400.16145671701879608No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15080.15810177224957433No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15050.15778724617745982No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14610.15317419711977992No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14530.1523354609274745No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14460.15160156675920722No Hit
GATTATTACTGCAGTTCATATACAACCAGCACCGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG14070.14751272782171823No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14060.14740788579768005No Hit
GTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGA14010.14688367567748914No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13860.14531104531691647No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13320.1396495760188548No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13000.13629463124963306No Hit
CTCCTGGACAGGCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT12790.1340929487448313No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12780.13398810672079312No Hit
GTGTATTACTGTGCGAGTAGCCTACCCATGATTGGCTACGATTCGACCTA12700.13314937052848766No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12390.1298992677833041No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12340.12937505766311322No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC12200.1279072693265787No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC12000.12581042884581511No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11370.11920538133140983No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCACCATCT11320.11868117121121893No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA11140.11679401477853171No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11080.11616496263430263No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10330.10830181083143918No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT10310.10809212678336282No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10210.10704370654298102No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10110.10599528630259926No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10110.10599528630259926No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9970.10452749796606474No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC9700.1016967633170339No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC9560.10022897498049939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.045.5405541
CTATATC457.436585E-540.31921864
GTATCAA30750.039.592541
TATATCT501.3517938E-436.42806665
TTAACGG807.496783E-834.28285635
GTCCGTC400.002801516833.9683382
TATCAAC36050.033.5443232
TAACCGT751.3961635E-632.94381353
AACGCAG36400.032.9435466
ATCAACG37250.032.370813
CAACGCA37500.032.066115
TCAACGC37750.031.8520814
TGGGGCA5650.031.60860670
CGCAGAG38450.031.2754768
AGAGTAC38950.030.87399511
GCAGAGT39200.030.6770949
CAGAGTA39350.030.64648410
TCTGGAG6150.030.11431370
GAGTTTA2600.030.0836091
ACGCAGA40550.029.5720147
Produced by FastQC (version 0.11.5)