FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846774
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACCAGTATGGTAGCTCACCTTGGACGTTCGGCCAAGGGA44290.5230439290767076No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36180.4272686690899815No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27230.3215734068358263No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24350.28756197049035515No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23440.276815301367307No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22950.27102863337797334No Hit
GTGTATTACTGTCACCAGTATGGTAGCTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA22900.27043815705253116No Hit
GTATGGTAGCTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT21420.2529600578194418No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20530.24244957922657048No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18760.22154671730591635No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18330.21646862090711336No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18090.21363433454499076No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT17930.2117448103035757No Hit
CTCCAGGCACGCTGGCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC16830.19875433114384713No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16790.19828195008349336No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCTCGGCAACTACTTAACCTGGTCCC16760.19792766428822803No Hit
GTCTCCAGGCACGCTGGCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCTCGG15940.18824385255097584No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15930.18812575728588737No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15920.18800766202079894No Hit
CTACTTAACCTGGTCCCAGCAGAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATCCAACAGGGCCA15220.17974099346460803No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15200.17950480293443116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15120.17856004081372362No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14700.1736000396800091No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14580.17218289649894777No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT14340.16934861013682517No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14320.1691124196066483No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14290.16875813381138297No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14210.16781337169067542No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13920.16438860900311061No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13650.1612000368457227No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12700.14998098666232076No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC12330.14561146185404844No Hit
CTACCATACTGGTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC12280.1450209855286062No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12040.1421866991664836No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11620.13722669803276907No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTCTGCTGGGACCAGGTTAAGTAGTTGCCGAGAACACTCTGA11540.13628193591206153No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCACCAGTATGGTAGCTCA11380.13439241167064647No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC11270.13309336375467362No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11200.13226669689905451No Hit
CCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG11050.13049526792272792No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10740.1268343147049862No Hit
GTCTCTGGTGGCTCCATCAACACTTACTATTGGAGCTGGATCCGGCAGCC10710.1264800289097209No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10540.1244724094032174No Hit
GTAATAAATGGCCGTGTCTGCGGCGGTCACAGAATTCAACTTCAGGGAGA10410.12293717095706765No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10360.12234669463162544No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10280.12140193251091791No Hit
GTCAGAGTGTTCTCGGCAACTACTTAACCTGGTCCCAGCAGAAACCTGGC9850.11632383611211493No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA9740.11502478819614206No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9670.11419812134052297No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9650.11396193081034608No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9640.11384383554525765No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAGATTGTGTTGACGCAGTC9610.11348954974999233No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAGATTGTGTTGACGCAGTCTC9470.11183621603875415No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9360.11053716812278128No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9280.10959240600207376No Hit
TCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9140.10793907229083557No Hit
ACTTAACCTGGTCCCAGCAGAAACCTGGCCAGCCTCCCAGGCTCCTCATC9020.10652192910977427No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8830.10427811907309388No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8690.10262478536185571No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAACACTTACTATTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA8680.10250669009676727No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8640.1020343090364135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT200.0072415351.9598542
GGTATCA9100.045.468861
GTATCAA22400.037.2487681
AATGGTA1801.9281288E-1036.73568770
AACTGTG20850.035.20283570
TATCAAC25950.032.3862
TACTGAT4500.032.32740870
CTAGCAC553.450247E-431.0556953
CGGAGTA450.00444573430.91132747
ATCAACG27150.030.8269213
TCAACGC27350.030.6014964
CAACGCA27700.030.3417435
AACGCAG27700.030.3417436
ACGCAGA29200.028.7830947
CGCAGAG30200.027.8300118
ATCCTAG500.00807554627.3564721
AGAGTAC30500.027.33223711
GCAGAGT30900.027.088999
CGCATTT659.102264E-426.3105222
ACTATTC700.001363609324.54876134
Produced by FastQC (version 0.11.5)