FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085659
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGACTCTCAGACTGTCCATTTGCAGATACA38760.35701817974152106No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30480.2807511382487503No Hit
ATATATTACTGTGCGAAACCCTTTACCAGTTCGGATTCATACTTTGACAC26390.24307816726983336No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24470.22539305619904593No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT21720.20006281898828268No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20940.1928782426157753No Hit
GTCTGAGAGTCGAGGACACGGCCATATATTACTGTGCGAAACCCTTTACCAGTTCGGATTCATACTTTGACAC20650.19020705396445844No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19930.1835751373129132No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT19330.1780485401032921No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19250.17731166047534264No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19160.17648267089389946No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18570.17104818363777208No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18070.16644268596308784No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17610.16220562810237837No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCACCAATGCCATGACCTGGGTCCGGCAGGCTCCAGGGAAGGGG17500.16119241861394784No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16910.15575793135782046No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16820.15492894177637728No Hit
GCCCTGGGATTGTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA16610.15299463275300992No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16330.15041555405518678No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16150.14875757489230043No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15730.1448889568455657No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15150.13954657954293198No Hit
CAGTAATATATGGCCGTGTCCTCGACTCTCAGACTGTCCATTTGCAGATA15080.1389018098684762No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15080.1389018098684762No Hit
GTATAGAAATTTCTGGGCGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT14790.13623062121715934No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14750.1358621814031846No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13970.1286776050306772No Hit
TCTATAAGGTGTCTACCTTACAAAGTGGGGTGCCGTCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACT13900.12803283535622142No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13650.12573008651887932No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAACAGTATAGAAATTT13540.12471687703044879No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13460.1239799974024993No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13440.12379577749551195No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13370.12315100782105615No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13070.12038770921624561No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13010.11983504949528352No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12910.11891394996034668No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12910.11891394996034668No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12790.11780863051842246No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12690.11688753098348562No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12580.11587432149505507No Hit
CCTTTAGCACCAATGCCATGACCTGGGTCCGGCAGGCTCCAGGGAAGGGGCTGGAGTGGGTGTCAGCCATTAGTG12580.11587432149505507No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12510.11522955182059928No Hit
ATATTACTGTGCGAAACCCTTTACCAGTTCGGATTCATACTTTGACACCTGGGGCCAGGGAGCCCTGGTCACCGT12450.11467689209963719No Hit
CCTTATAGATCAGGAGCTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTATTTATATTCTGA12370.11394001247168771No Hit
GAATATAAATAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAGGCCCCTAAGCTCCTGATCTATAAGGTGT12320.11347946270421928No Hit
CTCTCAGACTGTCCATTTGCAGATACAGGGTGTCCTTGGATGTGTCTCTG12190.11228203330880138No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12060.11108460391338348No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12010.11062405414591506No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12010.11062405414591506No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11750.10822919535507926No Hit
CCTTGGGGCTGGTCGGGGATGCTGATGAGACGGTGACCAGGGCTCCCTGG11690.10767653563411715No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11670.10749231572712979No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11620.10703176595966138No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11550.10638699628520558No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11440.10537378679677506No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11390.10491323702930663No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTTCTTGT11350.10454479721533189No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11350.10454479721533189No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11350.10454479721533189No Hit
CCCTGTATCTGCAAATGGACAGTCTGAGAGTCGAGGACACGGCCATATATTACTGTGCGAAACCCTTTACCAGTT11120.10242626828497715No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11050.10178149861052134No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG11040.10168938865702766No Hit
GCTTAGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCACCAATGCCATGA10980.10113672893606557No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10980.10113672893606557No Hit
TTTCTATACTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC10950.1008603990755845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3850.049.40526641
GCCTAGT400.00972971548.21639370
TCGTATG3800.048.16502440
ATGCCGT4050.047.17597244
GTATGCC4300.045.0985242
CGAGACG4150.044.97833623
ACGAGAC4150.044.97212222
ATCTCGT4050.044.09725637
CCGTCTT4200.044.0425747
ATCTCCG4400.043.170210
GCCGTCT4300.042.9653846
CCCACGA4350.042.88862619
CGTCCAA608.937662E-442.85901370
CACGAGA4400.042.4090721
CGTCTTC4500.041.973548
CATCTCG4250.041.96337536
TCTCCGA4450.041.92290511
CTACATC4600.041.59401333
GGAGCTA4450.041.28808629
CTTATAC4800.041.006771
Produced by FastQC (version 0.11.5)