FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085659
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGACTCTCAGACTGTCCATTTGCAGATACA34640.31906887890212304No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33090.30479183611060195No Hit
ATATATTACTGTGCGAAACCCTTTACCAGTTCGGATTCATACTTTGACAC28060.2584605295032787No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26120.24059119852550387No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25880.23838055964165544No Hit
GTCTGAGAGTCGAGGACACGGCCATATATTACTGTGCGAAACCCTTTACCAGTTCGGATTCATACTTTGACAC21620.19914171945334586No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21360.19674686066251004No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT19590.1804433988941279No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19140.17629845098691208No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17400.16027131907901102No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17100.15750802047420046No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCACCAATGCCATGACCTGGGTCCGGCAGGCTCCAGGGAAGGGG16670.15354729247397203No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16280.14995500428771835No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16020.14756014549688254No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15600.1436915274501478No Hit
GCCCTGGGATTGTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA15430.14212565824075515No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14940.1376122705195646No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14730.13567796149619724No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14610.13457264205427302No Hit
CAGTAATATATGGCCGTGTCCTCGACTCTCAGACTGTCCATTTGCAGATA14260.13134879368199406No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14010.12904604484465196No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13980.12876971498417092No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13900.12803283535622142No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13720.1263748561933351No Hit
GTATAGAAATTTCTGGGCGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT13720.1263748561933351No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13500.12434843721647404No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13410.12351944763503088No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13350.12296678791406879No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13140.12103247889070141No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13050.12020348930925825No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAACAGTATAGAAATTT13050.12020348930925825No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12940.11919027982082772No Hit
ATATTACTGTGCGAAACCCTTTACCAGTTCGGATTCATACTTTGACACCTGGGGCCAGGGAGCCCTGGTCACCGT12840.11826918028589087No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12730.11725597079746035No Hit
CCTTATAGATCAGGAGCTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTATTTATATTCTGA12700.1169796409369793No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12600.11605854140204246No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12330.11357157265771296No Hit
TCTATAAGGTGTCTACCTTACAAAGTGGGGTGCCGTCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACT12260.11292680298325716No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12050.1109924939598898No Hit
CCCTGTATCTGCAAATGGACAGTCTGAGAGTCGAGGACACGGCCATATATTACTGTGCGAAACCCTTTACCAGTT11710.10786075554110454No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11700.10776864558761083No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11540.1062948863317119No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11500.10592644651773715No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11460.10555800670376242No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11410.10509745693629399No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11170.10288681805244557No Hit
GAATATAAATAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAGGCCCCTAAGCTCCTGATCTATAAGGTGT11150.1027025981454582No Hit
CCTTTAGCACCAATGCCATGACCTGGGTCCGGCAGGCTCCAGGGAAGGGGCTGGAGTGGGTGTCAGCCATTAGTG11150.1027025981454582No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11050.10178149861052134No Hit
CCTTGGGGCTGGTCGGGGATGCTGATGAGACGGTGACCAGGGCTCCCTGG11050.10178149861052134No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11010.1014130587965466No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10860.10003140949414134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA200.00792664650.7862611
GTGGTAT3000.044.0593951
GTATCAA25650.036.7326851
GTACTAA3000.036.15131
GTCACCG13800.035.24083770
ACTAAGC3200.034.9155543
CGATAGA400.00266103234.32704537
TTAGACT400.002846931333.8575064
TACTAAG3250.033.3427622
CTAAGCC3650.031.5384984
AATGGTA2750.031.49293370
AACGCAG31300.030.2878636
TATCAAC31050.030.2101672
TAAGCCT3850.029.9001335
ATCAACG31400.029.8679283
CAACGCA31650.029.6320025
TCAACGC31900.029.3997754
TCTGGAG16900.028.77654370
GTGCTAT855.0848994E-627.910931
AGAGTAC34650.027.55502511
Produced by FastQC (version 0.11.5)