FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716259
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCGACAGTAAGAACTACTTAGCTTGGTTCCAGCAGAA28330.39552731623616594No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT27620.38561470082749394No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23480.32781437999382906No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22120.3088268349856686No Hit
ATATTATAGCAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19670.27462133111067366No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTACTGTCGGAGCTGTATAAAACACT19140.2672217731295523No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTAGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA18130.25312072867496255No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGCAATCCGGGGTCCCT17860.24935114253363658No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16340.22812976870098664No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15950.22268481094129358No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14980.2091422236928262No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14710.20537263755150023No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14400.2010445942040519No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14340.20020690839486835No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12380.17284250529487238No Hit
GCTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAAT12320.17200481948568883No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12140.16949176205813818No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12140.16949176205813818No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11900.166141018821404No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC11120.15525110330201786No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10680.1491080740346718No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9760.1362635582938574No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9700.13542587248467383No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC9440.13179590064487845No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9310.12998091472498077No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCTACTGCAAGTCCAGCCAGAGTGTTTTATA9310.12998091472498077No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9070.12663017148824657No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9030.1260717142821242No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8980.12537364277447124No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8960.12509441417141007No Hit
GTAATACACAGCCGTGTCTTCGGCTCTCAGATTGTTCATTTGCAGAAACA8820.12313981394998177No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8780.12258135674385942No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8760.12230212814079823No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8590.11992868501477817No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8300.11587987027039102No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGCAA8110.11322719854130978No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCTACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCGA7850.1095972267015144No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC7760.10834069798773907No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTACTGTCGGAGCTG7420.10359381173569895No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGAA7410.10345419743416837No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7250.1012203686096789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3850.050.3950921
GTATGCC4950.046.9166242
TGGTATC4300.046.6575432
TATCTCG4600.046.6485836
CGTATGC5100.046.07965541
GCCGTCT5050.045.1903646
CTTATAC5200.045.0314251
TACACAT5500.044.989185
ATCTCGT4800.044.8164337
GCCTATC4700.044.6645733
TCGTATG5250.044.6503440
CTATCTC4950.044.6375235
CCGGAGC5250.043.4861228
ATGCCGT5300.043.42516744
GACCGGA5250.043.40701726
GAGACCG5250.043.370624
TATACAC5500.043.1652953
ACCGGAG5300.043.0366627
CCTATCT4900.042.9234534
CGAGCCC5500.042.56327415
Produced by FastQC (version 0.11.5)