FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716259
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45320.6327320145366411No Hit
GTTTTATACAGCTCCGACAGTAAGAACTACTTAGCTTGGTTCCAGCAGAA22820.31859983609281No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22200.3099437493979133No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19490.272108273683123No Hit
ATATTATAGCAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT18460.2577280006254721No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTACTGTCGGAGCTGTATAAAACACT17440.2434873418693517No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17180.23985737002955634No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGCAATCCGGGGTCCCT16390.2288278402086396No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15100.2108175953111933No Hit
GCTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAAT14870.20760646637598967No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTAGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA14130.19727500806272594No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13400.18708316405099273No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12740.17786862014997368No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12600.1759140199285454No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11860.16558256161528162No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11730.16376757569538394No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11680.16306950418773097No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11560.16139413256936389No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11460.15999798955405797No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC11220.15664724631732377No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10680.1491080740346718No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10170.14198774465661165No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9360.13067898623263374No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9100.12704901439283836No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9070.12663017148824657No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8980.12537364277447124No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8910.1243963426637571No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8830.12327942825151236No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC8750.12216251383926766No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8630.12048714222090054No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCTACTGCAAGTCCAGCCAGAGTGTTTTATA8260.11532141306426866No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8250.11518179876273806No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTGCCGGGT8200.1144837272550851No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8020.11197066982753445No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7950.11099336971682032No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7900.11029529820916735No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG7760.10834069798773907No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG7710.1076426264800861No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7620.10638609776631078No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7400.10331458313263778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3850.041.6251831
GTATCAA32800.038.679911
GGTATCA12800.038.6035651
TGGTATC4450.038.239562
GACCGCG350.001586942838.1377687
TATCAAC38100.033.2783162
ATCAACG39450.031.9681263
TCAACGC39600.031.8470344
ACGATGT850.00394347331.67295570
AACGCAG39900.031.6142046
CAACGCA39900.031.6097895
ACGCAGA41250.030.6604567
CGCAGAG41900.030.1848168
AGAGTAC42600.029.61048511
CAGAGTA42800.029.4721210
GCAGAGT43200.029.199239
CGGGTAC1750.028.62931617
CGGGATC954.249123E-728.12704517
TAATTAG500.007618729527.68419341
CGGGCTA757.679331E-526.72069417
Produced by FastQC (version 0.11.5)