FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260684
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49490.3925646712419607No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41850.3319626488477684No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32240.2557341887419845No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30680.24335995380285622No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28770.22820944820430814No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27580.2187701279622808No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26620.21115521415358646No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGAGTCCCATCGCGGTTCAGTGCCAGTAGATCTGGGACAGATTTCA25530.20250911409996478No Hit
GTAGTATGTCGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGGCA24940.1978291149883714No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTGCAGGGAGA24910.1975911489318497No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24750.19632199663040065No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24480.19418030212170537No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24400.19354572597098083No Hit
GTACTGTAAGTCTGTAGACAGTAGTATGTCGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAAT24350.193149115876778No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT24180.19180064155648838No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23800.18878640484054687No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23660.18767589657677897No Hit
GACCAGGGAAAGCCCCTGAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGAGTCCCATCGCGGTTCAGTG23450.18601013418112708No Hit
CTTTTGGCCAGGGGACCAGGTTGGAGAGCAAACGAACTGTGGCTGCACCA22840.18117149103185254No Hit
GTTGGAGACAGAGTCTCCATCACTTGCCGGGCAAGTCAGACCATTAGCGACTATTTACATTGGTATCAACACAGA22470.1782365763347516No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22150.1756982717318535No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCTCCATCACTTGCCGGGCAAGTCAGACCATTAGCGA21980.17434979741156387No Hit
GTATCAACACAGACCAGGGAAAGCCCCTGAGCTCCTGATCTATGCTGCAT21890.1736358992419988No Hit
ACATACTACTGTCTACAGACTTACAGTACTTTGTACACTTTTGGCCAGGGGACCAGGTTGGAGAGCAAACGAA21730.17236674694054974No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21690.17204945886518747No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21160.1678453918666375No Hit
GTGTTGATACCAATGTAAATAGTCGCTAATGGTCTGACTTGCCCGGCAAG20720.16435522303765257No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20260.16070641017098655No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20240.16054776613330543No Hit
GTGGAGTCCCATCGCGGTTCAGTGCCAGTAGATCTGGGACAGATTTCACT20170.15999251200142145No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCGGATGACCCAGTC18730.14857014128837995No Hit
GGCCAAAAGTGTACAAAGTACTGTAAGTCTGTAGACAGTAGTATGTCGCA18240.14468336236519222No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17550.1392101430651932No Hit
ACACAGACCAGGGAAAGCCCCTGAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGAGTCCCATCGCGGTT17520.13897217700867148No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17420.13817895682026585No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTCTCCAACCTGGTCCCCTGGCCAAAAGTGTACAAAGTA17400.1380203127825847No Hit
GTGCAGCCACAGTTCGTTTGCTCTCCAACCTGGTCCCCTGGCCAAAAGTG17000.13484743202896204No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16890.13397488982171585No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16420.13024675493620924No Hit
CTTACAGTACTTTGTACACTTTTGGCCAGGGGACCAGGTTGGAGAGCAAA16340.1296121787854847No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16300.12929489071012243No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16250.12889828061591962No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15670.12429760352316678No Hit
CTCCATCACTTGCCGGGCAAGTCAGACCATTAGCGACTATTTACATTGGTATCAACACAGACCAGGGAAAGCCC15630.12398031544780452No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCGACATA15590.12366302737244225No Hit
ATACTACTGTCTACAGACTTACAGTACTTTGTACACTTTTGGCCAGGGGA15580.1235837053536017No Hit
GCATAGATCAGGAGCTCAGGGGCTTTCCCTGGTCTGTGTTGATACCAATG15460.1226318411275149No Hit
CTCCAACCTGGTCCCCTGGCCAAAAGTGTACAAAGTACTGTAAGTCTGTA15430.12239387507099321No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14940.11850709614780548No Hit
CAGTAGTATGTCGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGGCA14820.11755523192171868No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCGGATGACCCAGTCTCCATCCTCC14780.11723794384635641No Hit
GTCTACAGACTTACAGTACTTTGTACACTTTTGGCCAGGGGACCAGGTTGGAGAGCAAACGAACTGTGGCTGCAC14740.11692065577099414No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14590.11573082548838567No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14460.1146996392434583No Hit
AGGTTGGAGAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT14160.11231997867824134No Hit
GTCTATTACTGTGCGAGACGGACACACGTAGGCTTGAATTACTACTTTGA14070.11160608050867624No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13960.11073353830143003No Hit
GAGTGAAATCTGTCCCAGATCTACTGGCACTGAACCGCGATGGGACTCCA13810.10954370801882153No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13730.108909131868097No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13460.10676743735940172No Hit
TCCCTGGTCTGTGTTGATACCAATGTAAATAGTCGCTAATGGTCTGACTTGCCCGGCAAGTGATGGAGACT13310.10557760707679323No Hit
GTGCCAGTAGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCGACATACTA13200.104705064869547No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13050.10351523458693851No Hit
GTCTGCATCTGTTGGAGACAGAGTCTCCATCACTTGCCGGGCAAGTCAGA13020.10327726853041681No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT12880.10216676026664889No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12840.10184947219128662No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12720.10089760796519985No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12710.10081828594635928No Hit
GACTTACAGTACTTTGTACACTTTTGGCCAGGGGACCAGGTTGGAGAGCAAACGAACTGTGGCTGCACCAT12610.10002506575795363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)