FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260684
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51670.4098568713492041No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47670.3781280638129777No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38400.3045965523477731No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31480.2497057153101015No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30180.23939385286082795No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27940.22162572064054115No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25720.20401623245793554No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25270.20044674161011009No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24790.1966392847057629No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGAGTCCCATCGCGGTTCAGTGCCAGTAGATCTGGGACAGATTTCA24750.19632199663040065No Hit
GTAGTATGTCGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGGCA23790.1887070828217063No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTGCAGGGAGA23760.1884691167651846No Hit
GACCAGGGAAAGCCCCTGAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGAGTCCCATCGCGGTTCAGTG23470.1861687782188082No Hit
GTACTGTAAGTCTGTAGACAGTAGTATGTCGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAAT23430.18585149014344593No Hit
CTTTTGGCCAGGGGACCAGGTTGGAGAGCAAACGAACTGTGGCTGCACCA23210.1841064057289535No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22540.17879183046663558No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22220.17625352586373746No Hit
GTTGGAGACAGAGTCTCCATCACTTGCCGGGCAAGTCAGACCATTAGCGACTATTTACATTGGTATCAACACAGA21420.16990776435649219No Hit
ACATACTACTGTCTACAGACTTACAGTACTTTGTACACTTTTGGCCAGGGGACCAGGTTGGAGAGCAAACGAA21250.16855929003620257No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20910.16586234139562334No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20810.16506912120721767No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCTCCATCACTTGCCGGGCAAGTCAGACCATTAGCGA20660.16387929092460918No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20480.162451494585479No Hit
GTATCAACACAGACCAGGGAAAGCCCCTGAGCTCCTGATCTATGCTGCAT20270.1607857321898271No Hit
GTGTTGATACCAATGTAAATAGTCGCTAATGGTCTGACTTGCCCGGCAAG20230.16046844411446484No Hit
GTGGAGTCCCATCGCGGTTCAGTGCCAGTAGATCTGGGACAGATTTCACT20200.16023047805794316No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19790.15697827528547995No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19440.15420200462606012No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19440.15420200462606012No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCGGATGACCCAGTC18930.15015658166519127No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18910.14999793762751013No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17930.1422243797811347No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17720.1405586173854828No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17500.13881353297099033No Hit
GGCCAAAAGTGTACAAAGTACTGTAAGTCTGTAGACAGTAGTATGTCGCA17340.1375443806695413No Hit
ACACAGACCAGGGAAAGCCCCTGAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGAGTCCCATCGCGGTT17100.13564065221736774No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTCTCCAACCTGGTCCCCTGGCCAAAAGTGTACAAAGTA16870.1338162457840347No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16530.13111929714345547No Hit
CTCCATCACTTGCCGGGCAAGTCAGACCATTAGCGACTATTTACATTGGTATCAACACAGACCAGGGAAAGCCC16060.12739116225794886No Hit
CTTACAGTACTTTGTACACTTTTGGCCAGGGGACCAGGTTGGAGAGCAAA15780.12517014573041302No Hit
ATACTACTGTCTACAGACTTACAGTACTTTGTACACTTTTGGCCAGGGGA15740.12485285765505075No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15720.12469421361736963No Hit
GTGCAGCCACAGTTCGTTTGCTCTCCAACCTGGTCCCCTGGCCAAAAGTG15680.12437692554200735No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15610.1238216714101234No Hit
CAGTAGTATGTCGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGGCA15180.12041082459997907No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCGACATA15120.11993489248693567No Hit
GTCTACAGACTTACAGTACTTTGTACACTTTTGGCCAGGGGACCAGGTTGGAGAGCAAACGAACTGTGGCTGCAC14540.11533421539418284No Hit
CTCCAACCTGGTCCCCTGGCCAAAAGTGTACAAAGTACTGTAAGTCTGTA14470.11477896126229888No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14400.11422370713041491No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCGGATGACCCAGTCTCCATCCTCC14340.11374777501737153No Hit
AGGTTGGAGAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT14260.11311319886664699No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14170.11239930069708191No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14130.11208201262171964No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13860.10994031811302435No Hit
GCATAGATCAGGAGCTCAGGGGCTTTCCCTGGTCTGTGTTGATACCAATG13850.1098609960941838No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13610.1079572676420102No Hit
GAGTGAAATCTGTCCCAGATCTACTGGCACTGAACCGCGATGGGACTCCA13500.107084725434764No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13480.10692608139708284No Hit
GTCTGCATCTGTTGGAGACAGAGTCTCCATCACTTGCCGGGCAAGTCAGA13150.10430845477534417No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13140.10422913275650361No Hit
GTGCCAGTAGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCGACATACTA13120.10407048871882248No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12800.10153218411592436No Hit
GTCTATTACTGTGCGAGACGGACACACGTAGGCTTGAATTACTACTTTGA12730.1009769299840404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATT400.00906339649.09075570
GTGGTAT3300.038.0023161
TACTGAT11450.033.1559170
ATCAACG36150.030.161733
TACTACG450.00506299530.101672
TCAACGC36700.029.801984
CAACGCA36750.029.6716465
AACGCAG37450.029.30376
GATTCAC2350.028.845921
AATGGTA4650.028.15240570
ACGCAGA39250.027.8746457
CGCAGAG39700.027.5575928
AGAGTAC39850.027.19887411
GTCTACT8300.026.943867
GCAGAGT40850.026.6159659
GACGTGT8250.026.24986362
ACGTGTC8450.025.70354563
GTGTCTA8550.025.60048165
TTGGACG8750.024.95557659
TGGACGT9000.024.71503360
Produced by FastQC (version 0.11.5)