FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993941
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34000.34207261799241606No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33980.34187139880536166No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT33570.33774640547074725No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24120.242670339587561No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22190.22325268803681506No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22020.22154232494685297No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTA20030.20152101583494395No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19980.20101796786730802No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19680.19799968006149257No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19310.19427712510098688No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19230.19347224835276944No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19210.19327102916571506No Hit
GTCTGCATCTGTAGGAGACCGAGTCACCATCACTTGCCGGGCCAGTCAGA19110.19226493323044325No Hit
GTAGGAGACCGAGTCACCATCACTTGCCGGGCCAGTCAGAGAATTCCTGCCTGGTTGGCTTGGTATCAGCAGAAA18950.19065517973400836No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACCGAGTCACCATCACTTGCCG18820.189347255018155No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18010.18119787794245332No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17720.17828019973016507No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACCGAGTCACCATCACTTGCCGGGCCAGTCAGAGAATTCCTGC17130.17234423371206137No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16750.1685210691580285No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15830.15926498655352783No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC15470.1556430411865493No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15260.1535302397224785No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACAT15160.1525241437872067No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC15120.15212170541309797No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14910.15000890394902716No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTTTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14530.14618573939499427No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14380.14467659549208656No Hit
ATCTGAGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT14160.14246318443448858No Hit
CCTTTGGGGCCTTTCCTGGTTTCTGCTGATACCAAGCCAACCAGGCAGGA14070.14155769809274393No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13770.1385394102869285No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13440.1352192937005315No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13330.13411258817173252No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13270.13350893061056945No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13080.131597348333553No Hit
GAGTGAACTCTGTCTCAGATCCACCGCCGCTGAACCTTGACGGGACCCCACTCTCTAAAGTGGAAATCTTATAGA12910.1298869852435909No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12810.1288808893083191No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12490.12566138231544932No Hit
GTCTCAGATCCACCGCCGCTGAACCTTGACGGGACCCCACTCTCTAAAGT12470.12546016312839495No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12250.12324675207079697No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12060.1213351697937805No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACATCCAGCTCACCCAGTCTCCTTCCACC11910.11982602589087281No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11800.11871932036207382No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11720.11791444361385636No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAA11270.11338701190513321No Hit
CTCTAAAGTGGAAATCTTATAGACGAGGACCTTTGGGGCCTTTCCTGGTT11040.11107299125400803No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10940.11006689531873623No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10890.10956384735110031No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10430.10493580604884999No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10360.1042315388941597No Hit
CCATTATAGTTTTGGCAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACT10290.10352727173946945No Hit
ACTTATTACTGCCAAAACTATAATGGTTTTTCTCGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA10220.10282300458477917No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10160.1022193470236161No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10060.10121325108834428No Hit
GGACTGGAGTGGATTGGTTATATCTATTACAATGGCGTCACCAACTACAA9940.10000593596601809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)