FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993941
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58810.5916850195333526No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34790.35002077588106334No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30570.3075635274125929No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23870.2401550997493815No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23700.23844473665941943No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTA19480.19598748819094897No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18710.188240549489356No Hit
GTCTGCATCTGTAGGAGACCGAGTCACCATCACTTGCCGGGCCAGTCAGA18410.18522226168354058No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18390.1850210424964862No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18220.18331067940652412No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17950.18059422038129025No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17480.1758655694855127No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17140.17244484330558857No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACCGAGTCACCATCACTTGCCG17090.17194179533795265No Hit
GTAGGAGACCGAGTCACCATCACTTGCCGGGCCAGTCAGAGAATTCCTGCCTGGTTGGCTTGGTATCAGCAGAAA16990.17093569940268083No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16090.16188083598523453No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15830.15926498655352783No Hit
GAGTGAACTCTGTCTCAGATCCACCGCCGCTGAACCTTGACGGGACCCCACTCTCTAAAGTGGAAATCTTATAGA15540.15634730834123955No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACCGAGTCACCATCACTTGCCGGGCCAGTCAGAGAATTCCTGC15230.15322841094189696No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15130.15222231500662514No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14660.14749366411084763No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14530.14618573939499427No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTTTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14400.1448778146791409No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACAT14390.14477720508561373No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14160.14246318443448858No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14020.14105465012510804No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC13940.1402497733768906No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13860.13944489662867313No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13840.13924367744161875No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13720.1380363623192926No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC13650.13733209516460232No Hit
CCTTTGGGGCCTTTCCTGGTTTCTGCTGATACCAAGCCAACCAGGCAGGA13580.13662782800991205No Hit
ATCTGAGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT13000.13079247158533555No Hit
GTCTCAGATCCACCGCCGCTGAACCTTGACGGGACCCCACTCTCTAAAGT12770.1284784509342104No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12680.12757296459246575No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12640.12717052621835703No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12520.12596321109603084No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12440.1251583343478134No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11920.11992663548439997No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11780.11851810117501943No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACATCCAGCTCACCCAGTCTCCTTCCACC11480.11549981336920401No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11100.11167664881517113No Hit
GGGTGGAAGGAGACTGGGTGAGCTGGATGTCACAGTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG10980.11046933369284495No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAA10920.10986567613168186No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10850.1091614089769916No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10720.10785348426113822No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10660.10724982669997515No Hit
ACTTATTACTGCCAAAACTATAATGGTTTTTCTCGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA10560.10624373076470334No Hit
CTCTAAAGTGGAAATCTTATAGACGAGGACCTTTGGGGCCTTTCCTGGTT10470.1053382444229587No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10350.10413092930063254No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA10170.10231995661714327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG200.006207432654.02472357
GTACAAC200.00813013250.461316
GATTCAC1600.039.9827351
GTGGTAT3000.037.036641
GTATCAA20200.035.1698041
AATGGTA3450.033.2268870
CGTAGGA751.95971E-631.54415928
ACGTAGG751.976783E-631.50911927
ATCAACG22650.030.8900593
TATCAAC23100.030.433922
AACGCAG22950.030.1961636
TCAACGC23050.030.0621364
CAACGCA23150.029.9337835
GTAGTTT1603.2987573E-629.50107270
TTTATGA600.00590146729.17112769
ACGCAGA24600.028.170817
ATTCACT2300.027.7874932
AGAGTAC25300.027.6573211
CGCAGAG25150.027.554758
CAGAGTA25750.027.17398610
Produced by FastQC (version 0.11.5)