FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833572
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33570.4027246596574741No Hit
GCCTTGGGCTGACCTAGGACGGTCAATTTGGTCCCCCCGCCGAAAATCTTGAGACTGCTGGTGGTCGTATATGAG25560.30663218054349234No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24950.29931427639124153No Hit
GACCTAGGACGGTCAATTTGGTCCCCCCGCCGAAAATCTTGAGACTGCTG24380.2924762348063515No Hit
GTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG24100.2891171968348265No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19530.23429289851386562No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT19380.23249341388626296No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19120.2293743071984184No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18560.2226562312553685No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAATTTGGTCCCCC17640.21161939220607218No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17620.21137946092239182No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17490.20981990757846952No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17030.20430148805382137No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGCGACATT16900.2027419347098991No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16790.20142231264965713No Hit
ACTATAACCCGTCCCTCAAGAGTCGACTTACCATGTCGCTGGAGACGTCT16740.20082248444045625No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16650.19974279366389464No Hit
GTAATAAACGGCCGTGTCCGCGGCACTCACAGAGCTCATCTTCAGGGAGAACTGGTTCTTAGACGTCTCCAGCGA15880.19050543924220104No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15330.1839073289409913No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGCGACATTGGTCGTTATAATTATGTCTCCTGGTACCAACGGCG15320.18378736329915113No Hit
CTCCTGCACTGGAACCAGCAGCGACATTGGTCGTTATAATTATGTCTCCTGGTACCAACGGCGCCCAGACAAGGC15200.182347775597069No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14780.17730921863978155No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14500.1739501806682566No Hit
CTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14450.17335035245905572No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT14180.17011128012937093No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGGTGCCAGACTTGGAGCCAGAGAAGCGAGTAGAAACCCCTGAGGGCCGAA14130.16951145192017006No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14080.16891162371096918No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13800.16555258573944423No Hit
GTGTGGGGGCCTTGTCTGGGCGCCGTTGGTACCAGGAGACATAATTATAA13750.16495275753024333No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13580.16291334161896032No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13550.1625534446934398No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12910.15487564361566847No Hit
GGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGC12540.15043691486758193No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAATTTGGTCCCCCCGCCGAAAAT12470.14959715537470067No Hit
GGTTATAGTAGGTCGGGTTGTAAGAGATTCTCGCACTGTAATGGATGTAG12240.14683794561237662No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12170.1459981861194954No Hit
GTCTGGCACCACGGCCTCCCTGACCATCTCTGGACTCCAGGCTGAGGACG12110.14527839226845432No Hit
GTGCCAGACTTGGAGCCAGAGAAGCGAGTAGAAACCCCTGAGGGCCGAAC12090.14503846098477396No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11960.14347890764085167No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11950.14335894199901147No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11730.1407196978785276No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11620.13940007581828565No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11550.1385603163254044No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11450.13736065990700264No Hit
GTAATGGATGTAGCCAATCCACTCCAGGCCCTTCCCTGGGAGCTGGCGGA11130.13352175936811697No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT11050.13256203423339555No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10870.13040265268027237No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10510.126083889574026No Hit
GACAAGGCCCCCACACTCATCATCTATGATGTCAGTGTTCGGCCCTCAGG10320.12380454237906265No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10320.12380454237906265No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10310.12368457673722247No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10260.12308474852802158No Hit
CTCATCATCTATGATGTCAGTGTTCGGCCCTCAGGGGTTTCTACTCGCTT10200.1223649546769805No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10120.12140522954225909No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10060.12068543569121803No Hit
GTCTCCGGTGGCTCGATCAGTAATGGTAGTTACTACTGGAGCTGGATCCG10040.12044550440753767No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9940.1192458479891359No Hit
GTTTATTACTGTGCGAGGGAGTTCCTCGCGAATGAATTTGAAAAGAGTGG9730.11672656951049219No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9620.11540694745025026No Hit
GCTCATATACGACCACCAGCAGTCTCAAGATTTTCGGCGGGGGGACCAAATTGACCGTCCTAGGTCAGCCCAAGG9480.11372742846448777No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9320.11180797819504494No Hit
GATTATTTCTGCAGCTCATATACGACCACCAGCAGTCTCAAGATTTTCGG9280.11132811562768424No Hit
GTCACATCCTGGCTGGAATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9230.11072828741848335No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9210.110488356134803No Hit
ACCTAGGACGGTCAATTTGGTCCCCCCGCCGAAAATCTTGAGACTGCTGG9150.10976856228376193No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAATTTGGTCCCCCCGCCGAAAATCTTGAGACTG9060.10868887150720034No Hit
TCTATGATGTCAGTGTTCGGCCCTCAGGGGTTTCTACTCGCTTCTCTGGCTCCAAGTCTGGCACCACGGCCTCC9040.10844894022351999No Hit
GTAATGGTAGTTACTACTGGAGCTGGATCCGCCAGCTCCCAGGGAAGGGC9030.10832897458167981No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC8930.10712931816327803No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8870.10640952431223698No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8790.10544979917751557No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8580.10293052069887183No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8440.10125100171310937No Hit
TTATAATTATGTCTCCTGGTACCAACGGCGCCCAGACAAGGCCCCCACAC8420.101011070429429No Hit
ACCATGGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACT8420.101011070429429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3800.058.01595770
CGTATGC2650.045.6466241
TCGTATG2700.044.72475440
ATGCCGT2700.043.7324944
ATCTCGT2100.042.49624637
AAAGGGG3050.042.05526468
CACGAGA2950.041.39417321
GTGGTAT2150.041.0271951
CTTGAAA3050.040.93655457
TCTCGTA2200.040.64109438
GGTATCA7600.040.1759221
TGTCGTA602.9839794E-740.12005238
TGAGCGA3000.039.747731
AGACACT3100.039.4219825
CTTATAC3100.039.3983231
ACGAGAC3100.039.39359722
TATACAC3200.039.2108543
GAGCGAT2900.038.80143432
GTATCAA19450.038.5486871
CCGTCTT2900.038.51539647
Produced by FastQC (version 0.11.5)