FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833572
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33510.402004865806433No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC30350.3640957229849371No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25010.3000340702422826No Hit
GCCTTGGGCTGACCTAGGACGGTCAATTTGGTCCCCCCGCCGAAAATCTTGAGACTGCTGGTGGTCGTATATGAG24300.29151650967163No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT23530.2822791552499364No Hit
GTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG23120.27736056393448916No Hit
GACCTAGGACGGTCAATTTGGTCCCCCCGCCGAAAATCTTGAGACTGCTG20040.24041114624771465No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19160.22985416976577908No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18810.2256553723013729No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17220.20658083524878476No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17170.20598100703958386No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16940.2032217972772598No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16920.20298186599357945No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16720.20058255315677592No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGCGACATT16180.19410440849740634No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15730.18870595461459838No Hit
ACTATAACCCGTCCCTCAAGAGTCGACTTACCATGTCGCTGGAGACGTCT15700.18834605768907783No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAATTTGGTCCCCC15580.18690646998699573No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15150.18174794738786812No Hit
CTCCTGCACTGGAACCAGCAGCGACATTGGTCGTTATAATTATGTCTCCTGGTACCAACGGCGCCCAGACAAGGC14800.17754914992346194No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAATTTGGTCCCCCCGCCGAAAAT14370.17239062732433433No Hit
CTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14340.17203073039881378No Hit
GTAATAAACGGCCGTGTCCGCGGCACTCACAGAGCTCATCTTCAGGGAGAACTGGTTCTTAGACGTCTCCAGCGA14330.1719107647569736No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGGTGCCAGACTTGGAGCCAGAGAAGCGAGTAGAAACCCCTGAGGGCCGAA14130.16951145192017006No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGCGACATTGGTCGTTATAATTATGTCTCCTGGTACCAACGGCG13660.16387306675368174No Hit
GTGTGGGGGCCTTGTCTGGGCGCCGTTGGTACCAGGAGACATAATTATAA13660.16387306675368174No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13600.1631532729026407No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13480.16171368520055857No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13440.16123382263319785No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13400.16075396006583714No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13170.15799475030351307No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13030.1563152313177506No Hit
GTCTGGCACCACGGCCTCCCTGACCATCTCTGGACTCCAGGCTGAGGACG12360.14827753331445875No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12230.14671797997053643No Hit
GTGCCAGACTTGGAGCCAGAGAAGCGAGTAGAAACCCCTGAGGGCCGAAC12150.145758254835815No Hit
GGTTATAGTAGGTCGGGTTGTAAGAGATTCTCGCACTGTAATGGATGTAG12080.1449184953429338No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11900.1427591137898106No Hit
GGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGC11830.14191935429692937No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11660.13987993838564636No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11510.1380804537580437No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11030.1323221029497152No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11010.13208217166603484No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10810.1296828588292313No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10380.12452433623010371No Hit
GTAATGGATGTAGCCAATCCACTCCAGGCCCTTCCCTGGGAGCTGGCGGA10320.12380454237906265No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT10150.12176512646777964No Hit
CTCATCATCTATGATGTCAGTGTTCGGCCCTCAGGGGTTTCTACTCGCTT10110.12128526390041891No Hit
GTCTCCGGTGGCTCGATCAGTAATGGTAGTTACTACTGGAGCTGGATCCG9960.11948577927281627No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9890.11864601977993504No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC9840.11804619157073414No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9780.11732639771969307No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9670.11600677565945113No Hit
GACAAGGCCCCCACACTCATCATCTATGATGTCAGTGTTCGGCCCTCAGG9330.11192794383688512No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9280.11132811562768424No Hit
GCTCATATACGACCACCAGCAGTCTCAAGATTTTCGGCGGGGGGACCAAATTGACCGTCCTAGGTCAGCCCAAGG9230.11072828741848335No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9220.11060832177664318No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9180.11012845920928245No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9070.10880883714904051No Hit
ACCTAGGACGGTCAATTTGGTCCCCCCGCCGAAAATCTTGAGACTGCTGG8890.10664945559591733No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAATTTGGTCCCCCCGCCGAAAATCTTGAGACTG8710.10449007404279415No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8690.1042501427591138No Hit
GTTTATTACTGTGCGAGGGAGTTCCTCGCGAATGAATTTGAAAAGAGTGG8500.10197079556415042No Hit
GTAATGGTAGTTACTACTGGAGCTGGATCCGCCAGCTCCCAGGGAAGGGC8410.10089110478758882No Hit
ACCATGGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACT8370.10041124222022813No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT200.00787615250.8673824
GGTATCA7900.040.7552721
GTATCAA20650.039.5534441
GTGGTAT2300.038.3118251
AGATGAG6800.037.39113670
TGGTATC2500.035.2278482
TATCAAC23950.034.085082
GTATCAC502.0661698E-433.891231
TATACTG502.0712755E-433.8769955
ATCAACG23950.033.796133
AACGCAG24400.033.4624826
TCAACGC24750.032.97744
CAACGCA24750.032.8504185
ACGCAGA25800.031.6466887
CGCAGAG26150.031.223128
AGAGTAC26000.031.01234211
GCAGAGT26450.030.9970689
GACAGGT1750.030.9862671
GCTTGTA553.614495E-430.810211
ATACGTA553.6221332E-430.79911811
Produced by FastQC (version 0.11.5)