FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117143
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36850.32985929285686794No Hit
GGGTTACAGTATTCCGTGGACGTTCGGCCAGGGGACCAAAGTGGAAATCAGACGAACTGTGGCTGCACCAT34640.3100766866909608No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33730.30193090768146963No Hit
GTAATATATGGCCGTGTCGTCGACTCTCAGGCTGTCCATTTGCAGATACA28670.2566367958264967No Hit
GGACTGAACAGAGCGAACTCACCATGGAGTTTGGGCTGATCTGGCTTTTT27720.24813296059680812No Hit
CTCCTGTACAGCCTCTGGCTTCACATTTAGTGATTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG27510.2462531654407717No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24550.21975700514616303No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23680.21196928235686927No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22350.20006391303530524No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20510.18359332690622418No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20510.18359332690622418No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19850.17768539927296687No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19780.17705880088762138No Hit
ACAGTATTCCGTGGACGTTCGGCCAGGGGACCAAAGTGGAAATCAGACGA19580.1752685197866343No Hit
ATATATTACTGTGCGAGAGGGAGTAGGAATACGTTTGGGGGAGCTATTGG19480.17437337923614074No Hit
CTGTAACCCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACTCTGTC19070.17070330297911726No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGGGTTACAGTATT18730.16765982510743924No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18710.16748079699734053No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18430.16497440345595865No Hit
GTAGGAGACAGGGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG17820.15951404609794806No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17720.15861890554745453No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGGGTCACCATCACTT16030.14349103024411378No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16020.1434015161890644No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15770.14116366481283057No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC15080.1349871950144252No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14800.13248080147304328No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14640.13104857659225363No Hit
GTTTAGGGGCTTTCCCTGATTTATGCTGATACCAATTTAAATAACTGCTA14460.12943732360136528No Hit
GAATACTGTAACCCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACT14410.12898975332611848No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14300.1280050987205756No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14300.1280050987205756No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14150.1266623878948353No Hit
GCATAAATCAGGGAAAGCCCCTAAACTCCTCATCTATGCTGCAGTAAGTTTGCAAAGTGGGGTCCCATCCAGGTT13900.12442453651860146No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT13860.12406648029840406No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13690.12254474136256505No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13680.1224552273075157No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13570.1214705727019728No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACTTTGGTCCCCTGGCCGAACGTC13520.12102300242672603No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13500.12084397431662733No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12970.11609972939901159No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12950.11592070128891288No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT12940.11583118723386353No Hit
CCCCTAAACTCCTCATCTATGCTGCAGTAAGTTTGCAAAGTGGGGTCCCA12910.11556264506871546No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12790.11448847640812322No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12670.11341430774753097No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACTTTGGTCCCCTGGCCGAACGTCCACGGAATA12660.11332479369248162No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT12450.1114449985364452No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12350.11054985798595167No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12150.10875957688496458No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12090.10822249255466848No Hit
GAGTGAACTCTGTCCCAGATGCACTGCCACTGAACCTGGATGGGACCCCA12070.10804346444456976No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12000.10741686605922429No Hit
ACTTACTACTGTCAACAGGGTTACAGTATTCCGTGGACGTTCGGCCAGGGGACCAAAGTGGAAATCAGACGAA11940.10687978172892816No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11730.10499998657289174No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11490.10285164925170727No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11470.10267262114160855No Hit
CTCTTATTAGTGCAAGTGGTGTAACCACTTACTACGCAGACTCCGTGAAG11450.10249359303150984No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11330.10141942437091761No Hit
CAGTAATATATGGCCGTGTCGTCGACTCTCAGGCTGTCCATTTGCAGATA11290.10106136815072018No Hit
GTCCCAGATGCACTGCCACTGAACCTGGATGGGACCCCACTTTGCAAACT11270.10088234004062148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2600.045.17138741
TCGTATG2650.042.94920740
GTTAGCC405.4444274E-542.509853
GTGGTAT3700.042.300891
ATCTCCG2850.041.7659310
TATACAC3100.039.4930233
ATCTCGT2700.039.4450937
CACGAGA2850.039.38459421
CTTTTCG350.001427741638.96905529
GTATCAA28150.038.678091
ATGCCGT3050.038.67025844
ACGAGAC3100.038.40974822
TCTCCGA3050.037.91211711
ATACACA3400.037.008574
GTATGCC3200.036.75187742
CTACATC2900.036.5594333
CCCACGA3150.035.6304919
CATCTCG2900.035.5015436
TACACAT3550.035.4464155
CCGTCTT3250.035.40096747
Produced by FastQC (version 0.11.5)